For timber floors in residential buildings the vibrations can be annoying. In order to have a better understanding of the expected level of vibrations, more studies are needed where human response to vibration is evaluated. In the present study five wooden floors have been built in a laboratory hall. Test persons evaluated the vibrations for two situations, first by own walking on the floor and thereafter by sitting on a chair while a second person walked. The floors deflection and input accelerans were measured. The results from the measurements are compared to the test person’s answers. This test indicates that the first eigenfrequency is a better indicator for vibration discomfort than the broadband input accelerance.