The aim of this paper is to propose a framework for universal design of manual assembly workplaces. Workplaces that are adapted to different individuals, regardless of gender, language, background and functional variations, also support the fundamental goals of the Industry 5.0 concept and an accessible, safer, productive and error-proof work environment. The study included: 1) a qualitative study on key factors for universal design and comparison with universal design theory; 2) improvement suggestions to the company based on observations, interviews and theory; 3) a framework for universal design. The framework included the following areas: personalisation and context, activities/tasks and output, and methods/standards and factors were suggested so that companies could start their analysis work for the design. The study resulted in new insights and empirics regarding universal workplace design. [Submitted 13 November 2022; Accepted 13 April 2023]