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Applications of X-ray fluorescence microscopy with synchrotron radiation: From biology to materials science
RISE Research Institutes of Sweden, Materials and Production, Manufacturing Processes.ORCID iD: 0000-0002-1949-0877
Lund University, Sweden.
University of Oulu, Finland.
University of Oulu, Finland.
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2025 (English)In: Radiation Physics and Chemistry, ISSN 0969-806X, E-ISSN 1879-0895, Vol. 229, article id 112491Article in journal (Refereed) Published
Abstract [en]

X-ray fluorescence emission spectroscopy is a powerful tool to gain chemical information on a wide variety of samples. Its combination with focused X-ray beams and translation stages enables X-ray fluorescence microscopy, generating quantitative distribution maps for sets of chemical elements, depending on incident photon energy and detector specifications. The use of synchrotron radiation for X-ray fluorescence microscopy has led to unprecedented performance: with the advent of 4th generation synchrotron facilities such as MAX IV, the increase of the achievable incident photon flux has made higher sensitivity and measuring speed possible, while new nanofocus capabilities have enabled nanoscale spatial resolution. Here, an overview of recent and ongoing research is presented from selected two-dimensional X-ray fluorescence microscopy experiments carried out at NanoMAX, the hard X-ray nanoprobe beamline at MAX IV. Results showcase the technique’s versatility, as it is applied to microalgae, human dental tissue and engineered materials. 

Place, publisher, year, edition, pages
Elsevier Ltd , 2025. Vol. 229, article id 112491
Keywords [en]
Atomic emission spectroscopy; Chemical detection; Fluorescence microscopy; Nanoprobes; Radiation chemistry; Synchrotron radiation; Synchrotrons; X ray detectors; X ray microscopes; chlorophyll; hydroxyapatite; Chemical imaging; Chemical information; Distribution maps; Fluorescence emission spectroscopy; Material science; Nano scale; Translation stage; X ray fluorescence; X-ray beam; X-ray fluorescence microscopy; aquatic environment; Article; biocompatibility; breast feeding; cell membrane; chemical imaging; dinoflagellate; electromagnetism; electron microscopy; fluorescence microscopy; hypothesis; immobilization; microalga; multiphoton microscopy; nonhuman; photosynthesis; photothermal therapy; quality control; signal transduction; synchrotron radiation; transmission electron microscopy; X ray absorption spectroscopy; X ray fluorescence
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Physical Sciences
Identifiers
URN: urn:nbn:se:ri:diva-78060DOI: 10.1016/j.radphyschem.2024.112491Scopus ID: 2-s2.0-85213541360OAI: oai:DiVA.org:ri-78060DiVA, id: diva2:1950423
Note

Part of this research was funded by the Swedish Governmental Agency for Innovation Systems under contract 2020-03789. We acknowledge Maria Svensson Coelho for preparation of the microalgae, Jinhua Sun for the synthesis of the MXene used in the EMC coatings and Ulf Johansson for support during measurements on the EMC coatings. We acknowledge MAX IV Laboratory for time on Beamline NanoMAX under Proposals 20200566 (geopolymers), 20200605 (microalgae), 20200837 (dental tissue). Research conducted at MAX IV, a Swedish national user facility, is supported by the Swedish Research council under contract 2018-07152, the Swedish Governmental Agency for Innovation Systems under contract 2018-04969, and Formas under contract 2019-02496. S. S. acknowledges support by the Wenner-Gren Foundations under grant 2024-0093. M. P. acknowledges the University of Oulu and Research Council of Finland Profi5 - project 326291. K. R. acknowledges support by the Swedish Research Council contract 2017-03860.

Available from: 2025-04-07 Created: 2025-04-07 Last updated: 2025-09-23Bibliographically approved

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