Firefighters risk heat strain during occupational tasks when exposed to extremely hot environmental conditions and performing high-intensity work. Relevant training scenarios are there-fore essential. This study investigated the effect of a single simulated smoke dive and the following recovery phase on physiological and perceptual responses. Nineteen professional male firefighters (43 ± 8 yr) performed a 2-min stair walk and a15-min simulated smoke dive in a two-floor heat chamber (110°C to 272°C) (HEAT), followed by a 5-min stair walk outside the heat chamber. Heart rate (HR), gastrointestinal temperature (Tgi) and skin temperatures were registered continuously during the test. The Tgi increased significantly from the start (37.5 ± 0.3°C) to the end of HEAT (38.4 ± 0.4°C) and further increased after the heat exposure (39.6 ± 0.5°C). The HR also increased significantly from the start (92 ± 14 bpm) to the end of HEAT (185 ± 13 bpm) and increased after the heat exposure to a maximum of 190 ± 13 bpm. The simulated smoke dive induced high physiological strain on the firefighters, and the increase in Tgi and HR after the hot exposure must be considered during live fire events when repeated smoke dives are required.