Stereoscopic 3D TV viewing puts differentvisual demands on the viewer compared to 2D TV viewing.Previous research has reported on viewers’ fatigue anddiscomfort and other negative effects. This study is toinvestigate further how severe and what symptoms mayarise from somewhat longish 3D TV viewing. The MPEG3DV project is working on the next-generation videoencoding standard and in this process, MPEG issued a callfor proposal of encoding algorithms. To evaluate thesealgorithms a large scale subjective test was performedinvolving Laboratories all over the world [(MPEG 2011;Baroncini 2012)]. For the participating Labs, it wasoptional to administer a slightly modified Simulator SicknessQuestionnaire (SSQ) before and after the test. One ofthe SSQ data sets described in this article is coming fromthis study. The SSQ data from the MPEG test is the largestdata set in this study and also contains the longest viewingtimes. Along with the SSQ data from the MPEG test, wehave also collected questionnaire data in three other 3D TVstudies. We did two on the same 3D TV (passive filmpattern retarder) as in the MPEG test, and one was using aprojector system. As comparison SSQ data from a 2Dvideo quality experiment is also presented. This investigationshows a statistically significant increase in symptomsafter viewing 3D TV primarily related to the visual or Oculomotor system. Surprisingly, 3D video viewing usingprojectors did not show this effect.