The thermodynamics associated with the solvophobic effect in solutions of nonpolar molecules in formamide were esamined using high performance liquid chromatography (HPLC). The transfer of a serie of alkylbenzenes from forma mide to hydrocarbon environ ment was studied in the temperature range S80 °C in order to obtain the molar free energy, entropy, and enthalpy of transfer. The HPLC system consisted of poly(dimethylsilosane), PDMS, coated glass beads as the stationary phase and formamide as the mobile phase. The free energy and the enthalpy of transfer were found to be negative, while the entropy of transfer was positive. In general, the thermodynamic transfer quantities for formamide are both smaller in magnitude and les s tempe rature depe n de nt than those previou sly dete rm ined with water as the mobile phase. The relatively small ZxCp of transfer in the case of formamide indicates that there is negligible structuring of the formamide molecules around the nonpolar molecules, in contrast to the water case. The positive entropy of transfer in the case of formamide is attributed to comples formation between the alkylbenzenes and formamide. The driving force for the solvophobic effect seems to arise from the same source as the hydrophobic effect, namely the large cohesive energy of the solvent. This interpretation was used to explain differences in surfactant aggregation in formamide and water.