Several physico-chemical properties have been determined for, N-dodecyllactobionamide (LABA), maltose 6'-O-dodecanoate (C12-maltose ester), and tetra(ethylene oxide) dodecyl amide (TEDAd). Attractive headgroup interactions due to the increase in the flexibility of the sugar headgroup reduces the area/molecule at the liquid-vapor interface both below and above the critical micelle concentration (cmc). The obtained cmc:s were 0.35 mM (LABA), 0.3 mM (C12-maltose ester) and 0.5 mM (TEDAd). The monomer diffusion coefficient decreased with the molecular weight, and increasing headgroup flexibility of the sugar headgroup, and values were in the range from 3.1¥10-10-3.6¥10-10 m2/s. The micelle diffusion coefficients (0.46¥10-10-0.68¥10-10 m2/s) indicated that the TEDAd micelles deviated most from spherical shape. The micelle aggregation numbers determined by TRFQ were estimated to be 120±10 (LABA), 90±10 (C12-maltose ester) and 130±10 (TEDAd). The dynamic surface tension measurements show that the adsorption of TEDAd to the liquid-vapor interface at short surface life-times is diffusion limited, whereas an adsorption barrier is present for the sugar surfactants. The analysis of the dynamic surface tension data above the cmc shows that the rate of demicellization is faster for TEDAd than for the two sugar-based surfactants.