Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuumShow others and affiliations
2024 (English)In: International Journal of Biological Macromolecules, ISSN 0141-8130, E-ISSN 1879-0003, Vol. 267, article id 131229Article in journal (Refereed) Published
Abstract [en]
Biomass-derived oligo- and polysaccharides may act as elicitors, i.e., bioactive molecules that trigger plant immune responses. This is particularly important to increase the resistance of plants to abiotic and biotic stresses. In this study, cellulose nanofibrils (CNF) gels were obtained by TEMPO-mediated oxidation of unbleached and bleached kraft pulps. The molecular structures were characterized with ESI and MALDI MS. Analysis of the fine sequences was achieved by MS and MS/MS of the water-soluble oligosaccharides obtained by acid hydrolysis of the CNF gels. The analysis revealed the presence of two families: one corresponding to homoglucuronic acid sequences and the other composed by alternating glucose and glucuronic acid units. The CNF gels, alone or with the addition of the water-soluble oligosaccharides, were tested on Chili pepper (Capsicum annuum). Based on the characterization of the gene expression with Next Generation Sequencing (NGS) of the C. annuum’s total messenger RNA, the differences in growth of the C. annuum seeds correlated well with the downregulation of the pathways regulating photosynthesis. A downregulation of the response to abiotic factors was detected, suggesting that these gels would improve the resistance of the C. annuum plants to abiotic stress due to, e.g., water deprivation and cold temperatures.
Place, publisher, year, edition, pages
Elsevier B.V. , 2024. Vol. 267, article id 131229
Keywords [en]
Gels; Glucose; Nucleic Acids; Oligosaccharides; Plants; Resistance; Sequences; Water; Capsicum; Cellulose; Gene Expression Regulation, Plant; Nanofibers; Oligosaccharides; Gene expression; Glucose; Kraft pulp; Nanocellulose; Nanofibers; RNA; carboxylated cellulose nanofibrils; cellulose nanofiber; glucuronic acid; oligosaccharide; unclassified drug; cellulose; nanofiber; oligosaccharide; Abiotic stress; Capsicum annuum; Cellulose nanofibrils; Characterization; Elicitor; Genes expression; Nano-cellulose; Nano-fibrils; Plant substrate; TEMPO-oxidized nanofibril; anion exchange chromatography; Article; atomic force microscopy; Capsicum annuum; chemical structure; conductometry; differential gene expression; down regulation; drug analysis; electrospray; gel hydrolysis; gene expression; harvest; high performance liquid chromatography; high throughput sequencing; hydrolysis; mass spectrometry; matrix assisted laser desorption ionization time of flight mass spectrometry; matrix-assisted laser desorption-ionization mass spectrometry; nonhuman; nuclear magnetic resonance spectroscopy; oxidation; oxidative stress; pathway analysis; plant immunity; plant seed; RNA extraction; ultraviolet visible spectrophotometry; water deprivation; chemistry; drug effect; gene expression regulation; genetics; pepper; Gels
National Category
Biochemistry Molecular Biology
Identifiers
URN: urn:nbn:se:ri:diva-73284DOI: 10.1016/j.ijbiomac.2024.131229Scopus ID: 2-s2.0-85190721953OAI: oai:DiVA.org:ri-73284DiVA, id: diva2:1860645
Funder
The Research Council of Norway, 284300
Note
The Research Council of Norway (Grant no. 284300 ), ANR (France, Grant ANR-18-SUS2-0001 ), MINECO (Spain, Grant PCI2018-093114 ) and SUSFOOD2 ERA-NET program (Grant SPAREC) are acknowledged for funding. Mirjana Filipovic, Ingebjørg Leirset, Johnny Kvakland Melbø, Kenneth Aasarød (RISE PFI) and Simon Standoft (RISE) are acknowledged for excellent laboratory work.
2024-05-242024-05-242025-09-23Bibliographically approved