Determination of the chemical composition of biomaterial is important for their valued utilization in biorefinery. In this study, the chemical composition of five bamboo species, i.e., mitinga (Bambusa tulda), borak (Bambusa balcooa), rengoon (Thyrsostachys oliveri), orah (Dendrocalamus longispathus), and bajja (Bambusa vulgaris) were determined. The chemical characterization of these bamboo species can expedite a further study on the extraction of cellulose and lignin. alpha-cellulose content was in the range of 42.7-45.7% and Klason lignin content was 22.4-28.2%. The ash content was 1.8-4.3% for the studied five bamboo species. The alpha-cellulose and lignin content were similar to other non-timber spices. The ash content was lower than other non-timber species. Therefore, these species can be a potential source of raw material for biorefinery.