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  • 1.
    Arrhenius, Karine
    et al.
    RISE Research Institutes of Sweden, Materials and Production, Chemistry, Biomaterials and Textiles.
    Bohlen, Haleh
    RISE Research Institutes of Sweden, Materials and Production, Chemistry, Biomaterials and Textiles.
    Büker, Oliver
    RISE Research Institutes of Sweden, Safety and Transport, Measurement Technology.
    de Krom, Iris
    VSL Dutch Metrology Institute, Netherlands.
    Heikens, Dita
    VSL Dutch Metrology Institute, Netherlands.
    van Wijk, Janneke
    VSL Dutch Metrology Institute, Netherlands.
    Hydrogen purity analysis: Suitability of sorbent tubes for trapping hydrocarbons, halogenated hydrocarbons and sulphur compounds2020In: Applied Sciences, E-ISSN 2076-3417, Vol. 10, no 1, article id 120Article in journal (Refereed)
    Abstract [en]

    The ISO 14687-2 standard sets requirements for the purity of the hydrogen that is delivered at refuelling stations. These specifications cover a wide range of impurities and include challenging measurements, mainly due to the very low levels of the required detection limits and the need for "total" measurements (total hydrocarbons, total sulphur compounds, halogenated compounds). Most of the compounds belonging to the species are organic. Thermal desorption often coupled with gas chromatography is a common speciation method used to determine the content of organic impurities. However, no existing sorbent tubes are sufficiently universal to trap all possible impurities; depending on the sorbents and the sampling volume, some compounds may irreversibly adsorb or may break through. It is therefore necessary to evaluate sorbents for the compounds targeted at the level required. In this study, the suitability of sorbent tubes for trapping organic impurities in hydrogen was investigated. Suitable sorbents were selected based on a literature review of suitable sorbent materials. Short-term stability studies for compounds among hydrocarbons, halogenated compounds and sulphurcompounds on the selected sorbents have then been performed for storage periods of two weeks since this is the period typically required to complete the collection, transport and analysis of hydrogen samples. The study clearly shows that the method is promising for total species, even through the results show that not all of the compounds belonging to the three total species to be analysed when performing hydrogen purity analysis can be quantified on one unique sorbent. A multibed sorbent consisting of Tenax TA (weak), Carboxen 1003 (medium), Carbograph 1 (strong) is shown to be a versatile sorbent suitable for the three "total species"; only a few compounds from each family would need to be analysed using other analytical methods. This method proposed here for total species will not only provide a sum of concentrations, but also an identification of which compound(s) is/are actually present in the hydrogen.

  • 2.
    Jedvert, Kerstin
    et al.
    RISE Research Institutes of Sweden, Materials and Production, Chemistry, Biomaterials and Textiles.
    Idström, Alexander
    RISE Research Institutes of Sweden, Materials and Production.
    Köhnke, Tobias
    Alkhagen, Mårten
    RISE Research Institutes of Sweden, Materials and Production, Chemistry, Biomaterials and Textiles.
    Cellulosic nonwovens produced via efficient solution blowing technique2020In: Journal of Applied Polymer Science, ISSN 0021-8995, E-ISSN 1097-4628, Vol. 137, no 5, article id 48339Article in journal (Refereed)
    Abstract [en]

    The demand for nonwoven materials has increased during the last few years and is expected to increase further due to its use in a broad range of new application areas. Today, the majority of nonwovens are from petroleum-based resources but there is a desideratum to develop sustainable and competitive materials from renewable feedstock. In this work, renewable nonwovens are produced by solution blowing of dissolved cellulose using 1-ethyl-3-methylimidazolium acetate (EMIMAc) as solvent. Properties of cellulose solutions and process parameters, such as temperature, flow rate, air pressure, and distance to collector, are evaluated in respect to spinnability and material structural properties. Nonwovens with fiber diameters mainly in the micrometer range were successfully produced and it was shown that high temperature or low flow rate resulted in thinner fibers. The produced materials were stiffer (higher effective stress and lower strain) compared to commercial polypropylene nonwoven. © 2019 The Authors. Journal of Applied Polymer Science published by Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 48339. © 2019 The Authors.

  • 3.
    Kundrát, Martin
    et al.
    University of Pavol Jozef Šafárik, Slovakia.
    Rich, Thomas
    Melbourne Museum, Australia.
    Lindgren, Johan
    Lund University, Sweden.
    Sjövall, Peter
    RISE Research Institutes of Sweden, Materials and Production, Chemistry, Biomaterials and Textiles.
    Vickers-Rich, Patricia
    Swinburne University of Technology, Australia; Monash University, Australia.
    Chiappe, Luis
    Natural History Museum of Los Angeles County, US.
    Kear, Benjamin
    Uppsala University, Sweden.
    A polar dinosaur feather assemblage from Australia2020In: Gondwana Research, ISSN 1342-937X, E-ISSN 1878-0571, Vol. 80Article in journal (Refereed)
    Abstract [en]

    Exceptionally preserved Mesozoic feathered dinosaur fossils (including birds) are famous, but recognized from only very few localities worldwide, and are especially rare in the Southern Hemisphere. Here we report an assemblage of non-avian and avian dinosaur feathers from an Early Cretaceous polar (around 70°S) environment in what is now southeastern Australia. The recovered remains incorporate small (10–30 mm long) basal paravian-like tufted body feathers, open-vaned contour feathers, and asymmetrical bird-like wing feathers that possess high-angled barbs with possible remnants of barbicels — amongst the geologically oldest observed to date. Such morphological diversity augments scant skeletal evidence for a range of insulated non-avian theropods and birds inhabiting extreme southern high-latitude settings during the Mesozoic. Although some of these fossil feathers exhibit what may be residual patterning, most are uniformly toned and preserve rod-shaped microbodies, as well as densely-packed microbody imprints on the barbules that are structurally consistent with eumelanosomes. Geochemical analysis detected no identifiable residual biomolecules, which we suspect were lost via hydrolysis and oxidization during diagenesis and weathering. Nevertheless, an originally dark pigmentation can be reasonably inferred from these melanic traces, which like the coloured feathers of modern birds, might have facilitated crypsis, visual communication and/or thermoregulation in a cold polar habitat. 

  • 4.
    Landberg, Göran
    et al.
    University of Gothenburg, Sweden.
    Fitzpatrick, Paul
    University of Gothenburg, Sweden.
    Isakson, Pauline
    University of Gothenburg, Sweden.
    Jonasson, Emma
    University of Gothenburg, Sweden.
    Karlsson, Joakim
    University of Gothenburg, Sweden.
    Larsson, Erik
    University of Gothenburg, Sweden.
    Svanström, Andreas
    University of Gothenburg, Sweden.
    Rafnsdottir, Svanheidur
    University of Gothenburg, Sweden.
    Persson, Emma
    University of Gothenburg, Sweden.
    Gustafsson, Annna
    University of Gothenburg, Sweden.
    Andersson, Daniel
    University of Gothenburg, Sweden.
    Rosendahl, Jennifer
    RISE Research Institutes of Sweden, Materials and Production, Chemistry, Biomaterials and Textiles.
    Petronis, Sarunas
    RISE Research Institutes of Sweden, Materials and Production, Chemistry, Biomaterials and Textiles.
    Ranji, Parmida
    University of Gothenburg, Sweden.
    Gregersson, Pernilla
    University of Gothenburg, Sweden.
    Magnusson, Ylva
    University of Gothenburg, Sweden.
    Håkansson, Joakim
    RISE Research Institutes of Sweden, Materials and Production, Chemistry, Biomaterials and Textiles.
    Ståhlberg, Anders
    University of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden.
    Patient-derived scaffolds uncover breast cancer promoting properties of the microenvironment2020In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 235, article id 119705Article in journal (Refereed)
    Abstract [en]

    Tumor cells interact with the microenvironment that specifically supports and promotes tumor development. Key components in the tumor environment have been linked to various aggressive cancer features and can further influence the presence of subpopulations of cancer cells with specific functions, including cancer stem cells and migratory cells. To model and further understand the influence of specific microenvironments we have developed an experimental platform using cell-free patient-derived scaffolds (PDSs) from primary breast cancers infiltrated with standardized breast cancer cell lines. This PDS culture system induced a series of orchestrated changes in differentiation, epithelial-mesenchymal transition, stemness and proliferation of the cancer cell population, where an increased cancer stem cell pool was confirmed using functional assays. Furthermore, global gene expression profiling showed that PDS cultures were similar to xenograft cultures. Mass spectrometry analyses of cell-free PDSs identified subgroups based on their protein composition that were linked to clinical properties, including tumor grade. Finally, we observed that an induction of epithelial-mesenchymal transition-related genes in cancer cells growing on the PDSs were significantly associated with clinical disease recurrences in breast cancer patients. Patient-derived scaffolds thus mimics in vivo-like growth conditions and uncovers unique information about the malignancy-inducing properties of tumor microenvironment. © 2019 The Authors

  • 5.
    Monte, Joana
    et al.
    Universidade Nova de Lisboa, Portugal.
    Ribeiro, Claudia
    A4F Algae for Future, Portugal.
    Parreira, Celina
    A4F Algae for Future, Portugal.
    Costa, Luis
    A4F Algae for Future, Portugal.
    Brive, Lena
    RISE Research Institutes of Sweden, Materials and Production, Chemistry, Biomaterials and Textiles.
    Casal, Susana
    Universidade do Porto, Portugal.
    Brazinha, Carla
    Universidade Nova de Lisboa, Portugal.
    Crespo, Jaoa
    Universidade Nova de Lisboa, Portugal.
    Biorefinery of Dunaliella salina: Sustainable recovery of carotenoids, polar lipids and glycerol2020In: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 297, article id 122509Article in journal (Refereed)
    Abstract [en]

    Dunaliella salina is well-known for its high content in carotenoids and glycerol. Nevertheless, Dunaliella salina has also a high content in lipids, including polar lipids, which are suitable for nutraceutical/cosmeceutical applications. This work proposes a sustainable process to maximise the potential of Dunaliella salina for the production of distinct fractions of carotenoids, glycerol, polar lipids and proteins, which may contribute to improve the revenues of the microalgae industry. In this work, extraction with non-hazardous solvents and organic solvent nanofiltration are integrated, in order to obtain added-value products and glycerol. Also, aiming to separate carotenoids from glycerides, a saponification process is proposed. High overall recoveries were obtained for carotenoids (85%), glycerol (86%), polar lipids (94%) and proteins (95%). In order to evaluate the profitability of the proposed biorefinery, an economic assessment was accomplished. Both CAPEX and OPEX (Capital and Operating expenditure) were calculated, likewise the Return of Investment (ROI).

  • 6.
    Roos, Sandra
    et al.
    RISE Research Institutes of Sweden, Materials and Production, Product Realisation Methodology.
    Posner, Stefan
    Stefan Posner AB, Sweden.
    Jönsson, Christina
    RISE Research Institutes of Sweden, Materials and Production.
    Olsson, Elisabeth
    RISE Research Institutes of Sweden, Materials and Production, Chemistry, Biomaterials and Textiles.
    Linden, Hanna
    RISE Research Institutes of Sweden, Materials and Production, Product Realisation Methodology.
    Schellenberger, Steffen
    RISE Research Institutes of Sweden, Materials and Production, Product Realisation Methodology.
    Larsson, Mikael
    RISE Research Institutes of Sweden, Materials and Production, Product Realisation Methodology.
    Hanning, Anne-Charlotte
    RISE Research Institutes of Sweden, Materials and Production, Product Realisation Methodology.
    Arvidsson, Rickard
    Chalmers University of Technology, Sweden.
    A Function-Based Approach for Life Cycle Management of Chemicals in the Textile Industry2020In: Sustainability, ISSN 2071-1050, E-ISSN 2071-1050Article in journal (Refereed)
    Abstract [en]

    Consumer products such as clothes and footwear sometimes contain chemical substances with properties that pose a risk to human health and the environment. These substances, restricted by law or company policy, are in focus for chemicals management processes by textile retailers. However, complex and non-transparent supply chains, and limited chemical knowledge, makes chemicals management challenging. Therefore, a function-based approach for life cycle management (LCM) of chemicals was developed, based on results of previous projects and evaluated using a two-step Delphi process. The resulting approach aims to help retailers identify and substitute hazardous substances in products, and consists of three parts: (i) a function-based chemicals management concept model for different levels of chemical information within the supply chain, (ii) tools for non-chemists which explain chemical information, and (iii) a continuous provision of knowledge to stakeholders (e.g., retailers) in a network. This approach is successfully implemented by over 100 retailers in the Nordic countries, providing the textile industry with practical and robust tools to manage and substitute hazardous chemicals in products and production processes. We conclude that the developed approach provides an explicit link, communication, and knowledge sharing between actors in the supply chain, which has proven important in chemicals LCM.

  • 7.
    Tasiopoulos, Christos
    et al.
    KTH Royal Institute of Technology, Sweden.
    Petronis, Sarunas
    RISE Research Institutes of Sweden, Materials and Production, Chemistry, Biomaterials and Textiles.
    Sahlin, Herman
    Neoss Ltd, Sweden.
    Hedhammar, My
    KTH Royal Institute of Technology, Sweden.
    Surface Functionalization of PTFE Membranes Intended for Guided Bone Regeneration Using Recombinant Spider Silk2020In: ACS Applied Bio Materials, ISSN 2576-6422, Vol. 3, no 1, p. 577-583Article in journal (Refereed)
    Abstract [en]

    Alveolar bone loss is usually treated with guided bone regeneration, a dental procedure which utilizes a tissue-separation membrane. The barrier membrane prevents pathogens and epithelial cells to invade the bone augmentation site, thereby permitting osteoblasts to deposit minerals and build up bone. This study aims at adding bioactive properties to otherwise inert PTFE membranes in order to enhance cell adherence and promote proliferation. A prewetting by ethanol and stepwise hydration protocol was herein employed to overcome high surface tension of PTFE membranes and allow for a recombinant spider silk protein, functionalized with a cell-binding motif from fibronectin (FN-silk), to self-assemble into a nanofibrillar coating. HaCaT and U-2 OS cells were seeded onto soft and hard tissue sides, respectively, of membranes coated with FN-silk. The cells could firmly adhere as early as 1 h post seeding, as well as markedly grow in numbers when kept in culture for 7 days. Fluorescence and scanning electron microscopy images revealed that adherent cells could form a confluent monolayer and develop essential cell-cell contacts during 1 week of culture. Hence, functionalized PTFE membranes have a potential of better integration at the implantation site, with reduced risk of membrane displacement as well as exposure to oral pathogens.

  • 8.
    Tiemann, T T
    et al.
    University of Gothenburg, Sweden.
    Padma, A M
    University of Gothenburg, Sweden.
    Sehic, E
    University of Gothenburg, Sweden.
    Bäckdahl, Henrik
    RISE Research Institutes of Sweden, Materials and Production, Chemistry, Biomaterials and Textiles.
    Oltean, M
    University of Gothenburg, Sweden.
    Song, M J
    University of Gothenburg, Sweden; The Catholic University of Korea, South Korea.
    Brännström, M
    University of Gothenburg, Sweden; Stockholm IVF-EUGIN, Sweden.
    Hellström, M
    University of Gothenburg, Sweden.
    Towards uterus tissue engineering: a comparative study of sheep uterus decellularisation.2020In: Molecular human reproduction, ISSN 1360-9947, E-ISSN 1460-2407, Vol. 10, no 1Article in journal (Refereed)
    Abstract [en]

    Uterus tissue engineering may dismantle limitations in current uterus transplantation protocols. A uterine biomaterial populated with patient-derived cells could potentially serve as a graft to circumvent complicated surgery of live donors, immunosuppressive medication, and rejection episodes. Repeated uterine bioengineering studies on rodents have shown promising results using decellularised scaffolds to restore fertility in a partially impaired uterus, and now mandate experiments on larger and more human-like animal models. The aim of the presented studies was therefore to establish adequate protocols for scaffold generation, and prepare for future in-vivo sheep uterus bioengineering experiments. Three decellularisation protocols were developed using vascular perfusion through the uterine artery of whole sheep uteri obtained from slaughterhouse material. Decellularisation solutions used were based on 0.5% sodium dodecyl sulfate (protocol 1) or 2% sodium deoxycholate (protocol 2) or with a sequential perfusion of 2% sodium deoxycholate and 1% Triton X-100 (protocol 3). The scaffolds were examined by histology, extracellular matrix quantification, evaluation of mechanical properties and the ability to support fetal sheep stem cells after recellularisation. We showed that a sheep uterus can successfully be decellularised while maintaining a high integrity of the extracellular components. Uteri perfused with sodium deoxycholate (protocol 2) was the most favourable treatment in our study based on quantifications. However, all scaffolds supported stem cells for two weeks in vitro and showed no cytotoxicity signs. Cells continued to express markers for proliferation and maintained their undifferentiated phenotype. Hence, this study reports three valuable decellularisation protocols for future in-vivo sheep uterus bioengineering experiments.

  • 9.
    van der Veen, Ike
    et al.
    Vrije Universiteit, Netherlands.
    Hanning, Anne-Charlotte
    RISE Research Institutes of Sweden, Materials and Production, Product Realisation Methodology.
    Stare, Ann
    RISE Research Institutes of Sweden, Materials and Production, Chemistry, Biomaterials and Textiles.
    Leonards, Pim
    Vrije Universiteit, Netherlands.
    de Boer, Jacob
    Vrije Universiteit, Netherlands.
    Weiss, Jana
    Stockholm University, Sweden.
    The effect of weathering on per- and polyfluoroalkyl substances (PFASs) from durable water repellent (DWR) clothing2020In: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 249, article id 126100Article in journal (Refereed)
    Abstract [en]

    To assess the effects of weathering on per- and polyfluoroalkyl substances (PFASs) from durable water repellent (DWR) clothing, thirteen commercial textile samples were exposed to elevated ultra violet (UV) radiation, humidity, and temperature in an aging device for 300 h, which mimics the lifespan of outdoor clothing. Before and after aging, the textile samples were extracted and analysed for the ionic PFASs (perfluoroalkyl acids (PFAAs), perfluorooctane sulfonamide (FOSA)) and volatile PFASs (fluorotelomer alcohols (FTOHs), acrylates (FTACs) and methacrylates (FTMACs)). Results showed that weathering can have an effect on PFASs used in DWR of outdoor clothing, both on the PFAS profile and on the measured concentrations. In most weathered samples the PFAA concentrations increased by 5- to more than 100-fold, while PFAAs not detected in the original textiles were detected in the weathered samples. DWR chemistries are based on side-chain fluorinated polymers. A possible explanation for the increase in concentration of the PFAAs is hydrolysis of the fluorotelomer based polymers (FTPs), or degradation of the FTOHs, which are used in the manufacturing of the FTPs. The concentrations of volatile PFASs also increased, by a factor up to 20. Suggested explanations are the degradation of the DWR polymers, making non-extractable fluorines extractable, or the transformation or degradation of unknown precursors. Further research is needed to unravel the details of these processes and to determine the transformation routes. This study shows that setting maximum tolerance limits only for a few individual PFASs is not sufficient to control these harmful substances in outdoor clothing.

  • 10.
    Varmuza, Kurt
    et al.
    Vienna University of Technology, Austria.
    Filzmoser, Peter
    Vienna University of Technology, Austria.
    Fray, Nicolas
    Université de Paris, France.
    Cottin, Herve
    Université de Paris, France.
    Merouane, Siane
    Max-Planck-Institute, Germany.
    Stenzel, Oliver
    Max-Planck-Institute, Germany.
    Paquette, John
    Max-Planck-Institute, Germany.
    Kissel, Jochen
    Max-Planck-Institute, Germany.
    Briois, Christelle
    Université d'Orléans, France.
    Baklouti, Donia
    Université Paris Sud, France.
    Bardyn, Anais
    University of Maryland, USA.
    Siljeström, Sandra
    RISE Research Institutes of Sweden, Materials and Production, Chemistry, Biomaterials and Textiles.
    Silén, Johan
    Finnish Meteorological Institute, Finland.
    Hilchenbach, Martin
    Max-Planck-Institute, Germany.
    Composition of cometary particles collected during two periods of the Rosetta mission: multivariate evaluation of mass spectral data2020In: Journal of Chemometrics, ISSN 0886-9383, E-ISSN 1099-128X, article id e3218Article in journal (Refereed)
    Abstract [en]

    The instrument COSIMA (COmetary Secondary Ion Mass Analyzer) onboard of the European Space Agency mission Rosetta collected and analyzed dust particles in the neighborhood of comet 67P/Churyumov-Gerasimenko. The chemical composition of the particle surfaces was characterized by time-of-flight secondary ion mass spectrometry. A set of 2213 spectra has been selected, and relative abundances for CH-containing positive ions as well as positive elemental ions define a set of multivariate data with nine variables. Evaluation by complementary chemometric techniques shows different compositions of sample groups collected during two periods of the mission. The first period was August to November 2014 (far from the Sun); the second period was January 2015 to February 2016 (nearer to the Sun). The applied data evaluation methods consider the compositional nature of the mass spectral data and comprise robust principal component analysis as well as classification with discriminant partial least squares regression, k-nearest neighbor search, and random forest decision trees. The results indicate a high importance of the relative abundances of the secondary ions C+ and Fe+ for the group separation and demonstrate an enhanced content of carbon-containing substances in samples collected in the period with smaller distances to the Sun. © 2020 The Authors.

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