Endre søk
Begrens søket
1234 101 - 150 of 163
RefereraExporteraLink til resultatlisten
Permanent link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Treff pr side
  • 5
  • 10
  • 20
  • 50
  • 100
  • 250
Sortering
  • Standard (Relevans)
  • Forfatter A-Ø
  • Forfatter Ø-A
  • Tittel A-Ø
  • Tittel Ø-A
  • Type publikasjon A-Ø
  • Type publikasjon Ø-A
  • Eldste først
  • Nyeste først
  • Skapad (Eldste først)
  • Skapad (Nyeste først)
  • Senast uppdaterad (Eldste først)
  • Senast uppdaterad (Nyeste først)
  • Disputationsdatum (tidligste først)
  • Disputationsdatum (siste først)
  • Standard (Relevans)
  • Forfatter A-Ø
  • Forfatter Ø-A
  • Tittel A-Ø
  • Tittel Ø-A
  • Type publikasjon A-Ø
  • Type publikasjon Ø-A
  • Eldste først
  • Nyeste først
  • Skapad (Eldste først)
  • Skapad (Nyeste først)
  • Senast uppdaterad (Eldste først)
  • Senast uppdaterad (Nyeste først)
  • Disputationsdatum (tidligste først)
  • Disputationsdatum (siste først)
Merk
Maxantalet träffar du kan exportera från sökgränssnittet är 250. Vid större uttag använd dig av utsökningar.
  • 101.
    Magnusson, Bertil
    et al.
    RISE - Research Institutes of Sweden (2017-2019), Biovetenskap och material, Kemi och material.
    Örnemark, Ulf
    Metodvalidering – Handbok för laboratoriet: 3:e upplagan2017Rapport (Annet vitenskapelig)
    Abstract [sv]

    Handboken fokuserar på validering och verifiering av kvantitativa metoder och ger endast viss vägledning och referenser till motsvarande arbete för kvalitativa metoder.

    Efter inledningen behandlas grunderna för metodvalidering och den terminologi som används. Därefter beskrivs 1) hur man tar fram krav på mätningen och beskriver mätmetodens olika steg. 2) valideringsplanen 3) de vanligaste prestandaegenskaperna som kan ingå i valideringen och 4) utformningen av valideringsrapporten där viktiga delar utgörs av slutsatser om kraven är uppfyllda och planering av den fortlöpande kontrollen av metodens prestanda. När metoden varit i rutinmässigt bruk en längre tid är det lämpligt att uppdatera valideringsunderlaget och detta beskrivs i ett separat avsnitt.

    Till stöd för arbetet finns sex bilagor. Bilaga 1 är en mall för valideringsrapport och Bilagorna 2 och 3 är genomarbetade exempel med mallen som grund. Bilaga 4 rör utvärdering av metodprestanda genom jämförelser mellan laboratorier. I Bilaga 5 visas hur beräkningen av precisionsdata kan förenklas och i Bilaga 6 behandlas vissa aspekter av regressionsanalys.

    I denna handbok används en standardiserad metod för bestämning av ammoniumkväve som exempel. Vi visar med användningen av en mall hur man steg för steg dokumenterar krav, plan och resultat till en komplett valideringsrapport på följande sätt:

  • 102.
    Manusson, Bertil
    et al.
    RISE - Research Institutes of Sweden (2017-2019), Biovetenskap och material, Kemi och material. Trollboken AB, Sweden.
    Hovind, Håvard
    NIVA, Norway.
    Krysell, Mikael
    Swedish Agency for Marine and Water Management, Sweden.
    Lund, Ulla
    Eurofins AS, Denmark.
    Mäkinen, Irma
    SYKE, Finland.
    Внутренний контроль качества: Руководство для химическихлабораторий2007Rapport (Annet vitenskapelig)
    Abstract [ru]

    Цель Troll книги состоит в том, чтобы дать практическое руководство длявнутреннего контроля качества. Она написана для Вас – работающих с обычнымиопределениями в аналитической лаборатории.

    Первая версия Внутреннего Контроля качества [1] – Руководство по ВнутреннемуКонтролю Качества в Водных Лабораториях (скандинавское сотрудничество) былоподготовлено в 1984, и пересмотренная версия была напечатана в 1986 в Норвегии,хорошо известной под названием Trollboken [2]. Позже она была переведена на ряддругих языков, и широко использовалось как инструмент в обычных химическихлабораториях – особенно в экологических лабораториях. Эта новая версияРуководства - улучшенная и расширенная редакция, и ее цель – как и всегда - что этодолжен быть практический инструмент для аналитиков в их ежедневной работе саналитическими методами.

    [...]

  • 103.
    Mayers, Joshua
    et al.
    Chalmers University of Technology, Sweden.
    Vaiciulyte, Sigita
    Malmhall-Bah, Eric
    Chalmers University of Technology, Sweden.
    Alcaide-Sancho, Javier
    Chalmers University of Technology, Sweden; Catholic University of Valencia, Spain.
    Ewald, Stephanie
    Chalmers University of Technology, Sweden.
    Godhe, Anna
    University of Gothenburg, Sweden.
    Ekendahl, Susanne
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Albers, Eva
    Chalmers University of Technology, Sweden.
    Identifying a marine microalgae with high carbohydrate productivities under stress and potential for efficient flocculation2018Inngår i: Algal Research, ISSN 2211-9264, Vol. 31, s. 430-442Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Microalgal biomass represents a potential third generation feedstock that could be utilised as a source of carbohydrates for fermentative production of a range of platform biochemicals. Identifying microalgal strains with high biomass and carbohydrate productivities while also being amenable to downstream processes is key in improving the feasibility of these processes. Utilising marine microalgae capable of growing in seawater will decrease reliance on freshwater resources and improve the sustainability of production. This study screened several marine microalgae believed to accumulate carbohydrates to find new high performing strains. Four strains had high growth rates and accumulated carbohydrates > 35% DW under stress. The strain Chlorella salina demonstrated the highest biomass and carbohydrate productivity, and alkaline autoflocculation (4 mM NaOH) enabled biomass recoveries > 95% efficiency, resulting in an 8-10 x concentration of the culture. Under nutrient replete conditions, biomass productivity reached 0.6 g L-1 d(-1), significantly greater than that of nitrogen starved cultures. However, nitrogen starvation rapidly increased carbohydrate content to > 50% DW in 2 days, resulting in carbohydrate productivities > 0.20 g L-1 d(-1). Chlorella salina partitions the products of photosynthesis preferentially into carbohydrate synthesis under nitrogen starvation. A greater understanding of cellular physiology and carbon partitioning in response to nutrient stress will enable better control and optimisation of the bio-processes. This study has identified a potentially high performance marine microalga for carbohydrate production that is also amenable to low-cost harvesting.

  • 104.
    Medvedevskikh, Maria
    et al.
    UNIIM Ural Scientific Research Institute for Metrology, Russia.
    Krasheninina, Maria
    UNIIM Ural Scientific Research Institute for Metrology, Russia.
    Rego, Elaine C. P. D.
    INMETRO National Institute of Metrology, Quality and Technology, Brazil.
    Wollinger, Wagner
    INMETRO National Institute of Metrology, Quality and Technology, Brazil.
    Monteiro, Taina M.
    INMETRO National Institute of Metrology, Quality and Technology, Brazil.
    Carvalho, Lucas J. D.
    INMETRO National Institute of Metrology, Quality and Technology, Brazil.
    Garcia, Steve Ali Acco
    INACAL Instituto Nacional de Calidad, Peru.
    Haraldsson, Conny
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Rodriguez, M Alejandra
    INTI National Institute of Industrial Technology, Argentina.
    Rodriguez, Gabriella
    INTI National Institute of Industrial Technology, Argentina.
    Salvo, Karino
    LATU Laboratorio Tecnológico del Uruguay, Uruguay.
    Gavrilkin, Vladimir
    UkrCSM State Enterprise All-Ukrainian State Research and production Center of Standardization Metrology, Certification and Consumers' Rights Protection, Ukraine.
    Kulyk, Sergiy
    UkrCSM State Enterprise All-Ukrainian State Research and production Center of Standardization Metrology, Certification and Consumers' Rights Protection, Ukraine.
    Samuel, Laly
    MSL Measurement Standards Laboratory of New Zealand, New Zealand.
    Report of the CCQM-K130: Nitrogen mass fraction measurements in glycine2017Inngår i: Metrologia, ISSN 0026-1394, E-ISSN 1681-7575, Vol. 54, nr 1AArtikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Mass fraction of nitrogen is very important pointer because the results of these measurements are often used for determination of protein mass fraction that is an important indicator of the quality of the vast majority of food products and raw materials, in particular dry milk powder. Proteins-enzymes catalyze chemical reactions, protein along with fats and carbohydrates is one of the indicators characterizing the energy value of food, so its definition is mandatory for all food products. The aim of this key comparison CCQM-K130 and pilot study P166 is to support National Measurement Institutes (NMIs) and Designated Institutes (DIs) to demonstrate the validity of the procedures the employed for determination of nitrogen mass fraction in glycine. The study material for this key comparison and pilot study has been selected to be representative as one of the aminoacid-the simplest part of the protein. Glycine is an amino acid, single acid that does not have any isomers (melting point-290 °C; specific heat of evaporation-528,6 J/kg; specific melting heat-981,1 J/kg; pKa-2,34, molar mass-75,07 g/mol, density-1,607 g/cm3). Ural Scientific Research Institute for Metrology (UNIIM) acted as the coordinating laboratory of this comparison and pilot study. Eight NMIs participated in this key comparison and two NMIs participated in Pilot study. The results of Pilot study are excluded from the Report B.

  • 105.
    Moen, Ingrid
    et al.
    Oxy Solutions AS, Norway.
    Ugland, Hege
    Oxy Solutions AS, Norway.
    Strömberg, Niklas
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Sjöström, Eva
    RISE - Research Institutes of Sweden, Biovetenskap och material, Yta, process och formulering.
    Karlsson, Anders
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Ringstad, Lovisa
    RISE - Research Institutes of Sweden, Biovetenskap och material, Yta, process och formulering.
    Bysell, Helena
    RISE - Research Institutes of Sweden.
    Amiry‐Moghaddam, Mahmood
    Oxy Solutions AS, Norway ; University of Oslo, Norway.
    Hagleröd, Camilla
    Oxy Solutions AS, Norway.
    Development of a novel in situ gelling skin dressing: Deliveringhigh levels of dissolved oxygen at pH 5.52018Inngår i: Health Sci Rep., artikkel-id e57Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background and aims: Wound healing requires appropriate oxygen and pH levels.Oxygen therapy and pH‐modulating treatments have shown positive effects onwound healing. Thus, a dressing, which combines high levels of dissolved oxygen(DO) with the pH of intact skin, may improve wound healing. Our aims were to (1) formulatean in situ gelling dressing with high levels of DO and with the pH level of intactskin, (2) evaluate physical and chemical properties of the dressing, and (3) elucidatebasic effects of elevated levels of DO on human skin cells in vitro.Methods: A dressing was formulated with 15 to 16 wt% poloxamer 407, acetatebuffer, and oxygenated water. Stability of pH and DO, rheology, and shelf life wereanalysed. Furthermore, in vitro studies of the effect of increased levels of DO wereperformed.Results: An in situ gelling wound dressing, with a DO concentration rangingbetween 25 and 35 mg/L and a pH of 5.5, was formulated. The DO concentrationwas stable above 22 mg/L for at least 30 hours when applied on a surface at 35°Cand covered for directed diffusion into the intended wound area. At storage, thedressing had stable pH for 3 months and stable DO concentration over 30 mg/L for7 weeks. Increasing DO significantly enhanced intracellular ATP in human skin cells,without changing reactive oxygen species production, proliferation rate, or viability.Conclusion: The developed dressing may facilitate wound healing by deliveringcontrolled and stable oxygen levels, providing adjustable pH for optimized healing,and increasing intracellular ATP availability.

    Fulltekst (pdf)
    fulltext
  • 106.
    Moghaddam, Maziar Sedighi
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. KTH Royal Institute of Technology, Sweden.
    Van den Bulcke, Jan
    Ghent University, Belgium.
    Wålinder, Magnus E. P.
    KTH Royal Institute of Technology, Sweden.
    Claesson, Per M.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. KTH Royal Institute of Technology, Sweden.
    Van Acker, Joris
    Ghent University, Belgium.
    Swerin, Agne
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. KTH Royal Institute of Technology, Sweden.
    Microstructure of chemically modified wood using X-ray computed tomography in relation to wetting properties2017Inngår i: Holzforschung, ISSN 0018-3830, E-ISSN 1437-434X, Vol. 71, nr 2, s. 119-128Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    X-ray computed tomography (XCT) was utilized to visualize and quantify the 2D and 3D microstructure of acetylated southern yellow pine (pine) and maple, as well as furfurylated pine samples. The total porosity and the porosity of different cell types, as well as cell wall thickness and maximum opening of tracheid lumens were evaluated. The wetting properties (swelling and capillary uptake) were related to these microstructural characteristics. The data show significant changes in the wood structure for furfurylated pine sapwood samples, including a change in tracheid shape and filling of tracheids by furan polymer. In contrast, no such changes were noted for the acetylated pine samples at the high resolution of 0.8

  • 107.
    Molander, Marcus
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Jansson, Anna
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Sandström, Johan
    RISE - Research Institutes of Sweden, Säkerhet och transport, Safety.
    Development of condition monitoring methods for polymeric com-ponents including low dose rate radiation exposure2018Konferansepaper (Fagfellevurdert)
    Abstract [en]

    Different plastics, rubber and other polymer based materials are widely used in various applications in nuclear power plants and inside containments, e.g. cable jacketing/insulators, sealants, paint coatings, lubricants and greases. As any other material or component, polymers are susceptible to ageing. Ele-vated temperature, ionizing radiation and moisture are considered to be the most important ageing stressors and they tend to interact with the polymer structure in different ways. In addition to these ageing stressors, properties of polymer composition, e.g. crystallinity degree, amount of fillers and antioxidants, has an effect to the ageing behavior. Thus, the degradation mechanism can be quite complex.

    Proper ageing management procedures are based on knowledge on the ageing behavior and how to set correct requirements for the polymer components in use that they will endure their designed life-time. The ageing behavior needs to be known when polymer components are qualified. Accelerated ageing is used as part of the artificial ageing of the qualified polymer and the ageing mechanism should be the same as in the real service environment in order to yield in identical ageing conditions. Thus, the effects of dose rate and temperature to the ageing mechanism must be known as well as the synergistic effects rising from the simultaneous and/or sequential exposure to ionizing radiation and excess heat.

    In a previous feasibility study [2015:157]1 the acceptance criteria for functional properties for different polymers in system components was studied. The components were selected based on interviews with the five Nordic nuclear power plants. A need to study the polymer degradation, using low dose rates (20-50 Gy/h to total dose of 14 kGy) was identified since previous work described in literature often focused on using a high dose rate to achieve the life time dose during a short period of time to achieve a life time dose2. This may cause different degradation, compared to that obtained with a long exposure at a low dose rate, which is the case for many O-rings inside a Nuclear Power plant (NPP). Like the previous feasibility study this research project is performed in collaboration with the Nordic nuclear industry for in a longer perspective implementing the results.

    Rubber materials were tested for different properties such as compression set, stress relaxation, elon-gation at break, Oxidation Induction Temperature (T

    ox) and hardness after heat ageing and gamma irradiation. These properties were then correlated to the function of the O-ring i.e. tightness verified by a leakage test in a specially designed test rig. To be able to better compare the degradation effect caused by the irradiation, a parallel test on samples aged in heat only was performed. By testing the correlation between material data and tightness of an O-ring an understanding of the function (tight-ness) based on a material property (compression set) can be made. The aim was to be able to use this to set acceptance criteria for an O-ring using compression set as a property. A model using Finite Elements (FE) based on the relaxation data and compression set data from the experiment was also done but is only described briefly in this paper.

  • 108.
    Moodie, Lindon W. K.
    et al.
    UiT The Arctic University of Norway, Norway; Umeå University, Sweden.
    Cervin, Gunnar
    University of Gothenburg, Sweden.
    Trepos, Rozenn
    Université de Bretagne Occidentale, France.
    Labriere, Christophe
    UiT The Arctic University of Norway, Norway.
    Hellio, Claire
    Université de Bretagne Occidentale, France.
    Pavia, Henrik
    University of Gothenburg, Sweden.
    Svenson, Johan
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. UiT The Arctic University of Norway, Norway.
    Design and Biological Evaluation of Antifouling Dihydrostilbene Oxime Hybrids2018Inngår i: Marine Biotechnology, ISSN 1436-2228, E-ISSN 1436-2236, Vol. 20, nr 2, s. 257-267Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    By combining the recently reported repelling natural dihydrostilbene scaffold with an oxime moiety found in many marine antifoulants, a library of nine antifouling hybrid compounds was developed and biologically evaluated. The prepared compounds were shown to display a low antifouling effect against marine bacteria but a high potency against the attachment and growth of microalgae down to MIC values of 0.01 μg/mL for the most potent hybrid. The mode of action can be characterized as repelling via a reversible non-toxic biostatic mechanism. Barnacle cyprid larval settlement was also inhibited at low μg/mL concentrations with low levels or no toxicity observed. Several of the prepared compounds performed better than many reported antifouling marine natural products. While several of the prepared compounds are highly active as antifoulants, no apparent synergy is observed by incorporating the oxime functionality into the dihydrostilbene scaffold. This observation is discussed in light of recently reported literature data on related marine natural antifoulants and antifouling hybrids as a potentially general strategy for generation of improved antifoulants. 

    Fulltekst (pdf)
    fulltext
  • 109.
    Moodie, Lindon W. K.
    et al.
    University of Umeå, Sweden .
    Sepčić, Kristina
    University of Ljubljana, Slovenia .
    Turk, Tom
    University of Ljubljana, Slovenia .
    FrangeŽ, Robert
    University of Ljubljana, Slovenia .
    Svenson, Johan
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Natural cholinesterase inhibitors from marine organisms2019Inngår i: Natural product reports (Print), ISSN 0265-0568, E-ISSN 1460-4752, Vol. 36, nr 8, s. 1053-1092Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Covering: Published between 1974 up to 2018Inhibition of cholinesterases is a common approach for the management of several disease states. Most notably, cholinesterase inhibitors are used to alleviate the symptoms of neurological disorders like dementia and Alzheimer's disease and treat myasthenia gravis and glaucoma. Historically, most drugs of natural origin have been isolated from terrestrial sources and inhibitors of cholinesterases are no exception. However, the last 50 years have seen a rise in the quantity of marine natural products with close to 25 000 reported in the scientific literature. A number of marine natural products with potent cholinesterase inhibitory properties have also been reported; isolated from a variety of marine sources from algae to ascidians. Representing a diverse range of structural classes, these compounds provide inspirational leads that could aid the development of therapeutics. The current paper aims to, for the first time, comprehensively summarize the literature pertaining to cholinesterase inhibitors derived from marine sources, including the first papers published in 1974 up to 2018. The review does not report bioactive extracts, only isolated compounds, and a specific focus lies on compounds with reported dose-response data. In vivo and mechanistic data is included for compounds where this is reported. In total 185 marine cholinesterase inhibitors and selected analogs have been identified and reported and some of the compounds display inhibitory activities comparable or superior to cholinesterase inhibitors in clinical use.

  • 110.
    Moodie, Lindon W. K.
    et al.
    UiT The Arctic University of Norway, Norway.
    Trepos, Rozenn
    Université de Bretagne Occidentale, France.
    Cervin, Gunnar
    University of Gothenburg, Sweden.
    Brathen, Kari Anne
    UiT The Arctic University of Norway, Norway.
    Lindgard, Bente
    UiT The Arctic University of Norway, Norway.
    Reiersen, Rigmor
    UiT The Arctic University of Norway, Norway.
    Cahill, Patrick
    Cawthron Institute, New Zealand.
    Pavia, Henrik
    University of Gothenburg, Sweden.
    Hellio, Clair
    Université de Bretagne Occidentale, France.
    Svenson, Johan
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. UiT The Arctic University of Norway, Norway.
    Prevention of Marine Biofouling Using the Natural Allelopathic Compound Batatasin-III and Synthetic Analogues2017Inngår i: Journal of Natural Products, ISSN 0163-3864, E-ISSN 1520-6025, Vol. 80, nr 7, s. 2001-2011Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The current study reports the first comprehensive evaluation of a class of allelopathic terrestrial natural products as antifoulants in a marine setting. To investigate the antifouling potential of the natural dihydrostilbene scaffold, a library of 22 synthetic dihydrostilbenes with varying substitution patterns, many of which occur naturally in terrestrial plants, were prepared and assessed for their antifouling capacity. The compounds were evaluated in an extensive screen against 16 fouling marine organisms. The dihydrostilbene scaffold was shown to possess powerful general antifouling effects against both marine microfoulers and macrofoulers with inhibitory activities at low concentrations. The species of microalgae examined displayed a particular sensitivity toward the evaluated compounds at low ng/mL concentrations. It was shown that several of the natural and synthetic compounds exerted their repelling activities via nontoxic and reversible mechanisms. The activities of the most active compounds such as 3,5-dimethoxybibenzyl (5), 3,4-dimethoxybibenzyl (9), and 3-hydroxy-3′,4,5′-trimethoxybibenzyl (20) were comparable to the commercial antifouling booster biocide Sea-nine, which was employed as a positive control. The investigation of terrestrial allelopathic natural products to counter marine fouling represents a novel strategy for the design of "green" antifouling technologies, and these compounds offer a potential alternative to traditional biocidal antifoulants.

  • 111.
    Moodie, Lindon W. K.
    et al.
    UiT The Arctic University of Norway, Norway.
    Trepos, Rozenn
    Université de Bretagne Occidentale, France.
    Cervin, Gunnar
    University of Gothenburg, Sweden.
    Larsen, Lesley
    University of Otago, New Zealand.
    Larsen, David S.
    University of Otago, New Zealand.
    Pavia, Henrik
    University of Gothenburg, Sweden.
    Hellio, Claire
    Université de Bretagne Occidentale, France.
    Cahill, Patrick
    Cawthron Institute, New Zealand.
    Svenson, Johan
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi, material och ytor. UiT The Arctic University of Norway, Norway.
    Probing the Structure-Activity Relationship of the Natural Antifouling Agent Polygodial against both Micro- and Macrofoulers by Semisynthetic Modification2017Inngår i: Journal of natural products (Print), ISSN 0163-3864, E-ISSN 1520-6025, Vol. 80, nr 2, s. 515-525Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The current study represents the first comprehensive investigation into the general antifouling activities of the natural drimane sesquiterpene polygodial. Previous studies have highlighted a high antifouling effect toward macrofoulers, such as ascidians, tubeworms, and mussels, but no reports about the general antifouling effect of polygodial have been communicated before. To probe the structural and chemical basis for antifouling activity, a library of 11 polygodial analogues was prepared by semisynthesis. The library was designed to yield derivatives with ranging polarities and the ability to engage in both covalent and noncovalent interactions, while still remaining within the drimane sesquiterpene scaffold. The prepared compounds were screened against 14 relevant marine micro- and macrofouling species. Several of the polygodial analogues displayed inhibitory activities at sub-microgram/mL concentrations. These antifouling effects were most pronounced against the macrofouling ascidian Ciona savignyi and the barnacle Balanus improvisus, with inhibitory activities observed for selected compounds comparable or superior to several commercial antifouling products. The inhibitory activity against the microfouling bacteria and microalgae was reversible and significantly less pronounced than for the macrofoulers. This study illustrates that the macro- and microfoulers are targeted by the compounds via different mechanisms.

  • 112.
    Murase, Kohei
    et al.
    BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, Sweden.
    Stenlund, Patrik
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, Sweden ; University of Gothenburg, Sweden.
    Thomsen, Peter
    BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, Sweden.
    Lausmaa, Jukka
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, Sweden.
    Palmquist, Anders
    BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, Sweden ; University of Gothenburg, Sweden.
    Three-dimensional modeling of removal torque and fracture progression around implants.2018Inngår i: Journal of materials science. Materials in medicine, ISSN 0957-4530, E-ISSN 1573-4838, Vol. 29, nr 7, artikkel-id 104Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In the present study, a model for simulations of removal torque experiments was developed using finite element method. The interfacial retention and fracturing of the surrounding material caused by the surface features during torque was analyzed. It was hypothesized that the progression of removal torque and the phases identified in the torque response plot represents sequential fractures at the interface. The 3-dimensional finite element model fairly accurately predicts the torque required to break the fixation of acid-etched implants, and also provides insight to how sequential fractures progress downwards along the implant side.

    Fulltekst (pdf)
    fulltext
  • 113.
    Murugan, A.
    et al.
    NPL National Physical Laboratory, United Kingdom.
    de Huu, M.
    METAS Federal Institute of Metrology, Sweden.
    Bacquart, T.
    NPL National Physical Laboratory, United Kingdom.
    van Wijk, J.
    VSI, The Netherlands.
    Arrhenius, Karine
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    te Ronde, I.
    NEN, The Netherlands.
    Hemfrey, D.
    NPL National Physical Laboratory, United Kingdom.
    Measurement challenges for hydrogen vehicles2019Inngår i: International journal of hydrogen energy, ISSN 0360-3199, E-ISSN 1879-3487, Vol. 44, nr 35, s. 19326-19333Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Uptake of hydrogen vehicles is an ideal solution for countries that face challenging targets for carbon dioxide reduction. The advantage of hydrogen fuel cell electric vehicles is that they behave in a very similar way to petrol engines yet they do not emit any carbon containing products during operation. The hydrogen industry currently faces the dilemma that they must meet certain measurement requirements (set by European legislation) but cannot do so due to a lack of available methods and standards. This paper outlines the four biggest measurement challenges that are faced by the hydrogen industry including flow metering, quality assurance, quality control and sampling.

  • 114.
    Nilebäck, Linnea
    et al.
    KTH Royal Institute of Technology, Sweden.
    Hedin, Jesper
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Widhe, Mona
    KTH Royal Institute of Technology, Sweden.
    Floderus, Lotta S
    KTH Royal Institute of Technology, Sweden.
    Krona, Annika
    RISE - Research Institutes of Sweden, Biovetenskap och material, Jordbruk och livsmedel.
    Bysell, Helena
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Hedhammar, My
    KTH Royal Institute of Technology, Sweden.
    Self-Assembly of Recombinant Silk as a Strategy for ChemicalFree Formation of Bioactive Coatings – a Real-Time Study2017Inngår i: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 18, nr 3, s. 846-854Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Functionalization of biomaterials with biologically active peptides can improve their performance after implantation. By genetic fusion to self-assembling proteins, the functional peptides can easily be presented on different physical formats. Herein, a chemical-free coating method based on self-assembly of the recombinant spider silk protein 4RepCT is described and used to prepare functional coatings on various biomaterial surfaces. The silk assembly was studied in real-time, revealing occurrence of continuous assembly of silk proteins onto surfaces and formation of nanofibrillar structures. The adsorbed amounts and viscoelastic properties were evaluated, and the coatings were shown to be stable against wash with hydrogen chloride, sodium hydroxide, and ethanol. Titanium, stainless steel, and hydroxyapatite were coated with silk fused to an antimicrobial peptide or a motif from fibronectin. Human primary cells cultured on the functional silk coatings show good cell viability and proliferation, implying potential to improve implant performance and acceptance by the body.

  • 115.
    Nilebäck, Linnea
    et al.
    KTH Royal Institute of Technology, Sweden.
    Widhe, Mona
    KTH Royal Institute of Technology, Sweden.
    Seijsing, Johan
    Stockholm University, Sweden.
    Bysell, Helena
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Sharma, Prashant K
    University of Groningen, Netherlands; University Medical Center of Groningen, Netherlands.
    Hedhammar, My
    KTH Royal Institute of Technology, Sweden.
    Bioactive Silk Coatings Reduce the Adhesion of Staphylococcus aureus while Supporting Growth of Osteoblast-like Cells.2019Inngår i: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 11, nr 28, s. 24999-25007Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Orthopedic and dental implants are associated with a substantial risk of failure due to biomaterial-associated infections and poor osseointegration. To prevent such outcomes, a coating can be applied on the implant to ideally both reduce the risk of bacterial adhesion and support establishment of osteoblasts. We present a strategy to construct dual-functional silk coatings with such properties. Silk coatings were made from a recombinant partial spider silk protein either alone (silkwt) or fused with a cell-binding motif derived from fibronectin (FN-silk). The biofilm-dispersal enzyme Dispersin B (DspB) and two peptidoglycan degrading endolysins, PlySs2 and SAL-1, were produced recombinantly. A sortase recognition tag (SrtTag) was included to allow site-specific conjugation of each enzyme onto silkwt and FN-silk coatings using an engineered variant of the transpeptidase Sortase A (SrtA*). To evaluate bacterial adhesion on the samples, Staphylococcus aureus was incubated on the coatings and subsequently subjected to live/dead staining. Fluorescence microscopy revealed a reduced number of bacteria on all silk coatings containing enzymes. Moreover, the bacteria were mobile to a higher degree, indicating a negative influence on the bacterial adhesion. The capability to support mammalian cell interactions was assessed by cultivation of the osteosarcoma cell line U-2 OS on dual-functional surfaces, prepared by conjugating the enzymes onto FN-silk coatings. U-2 OS cells could adhere to silk coatings with enzymes and showed high spreading and viability, demonstrating good cell compatibility.

  • 116.
    Nilsson, Fritjof
    et al.
    KTH Royal Institute of Technology, Sweden.
    Moyassari, Ali
    KTH Royal Institute of Technology, Sweden.
    Bautista, Angela
    E2F Electroficación y Estudios Ferroviarios, Spain.
    Castro, Abraham
    E2F Electroficación y Estudios Ferroviarios, Spain.
    Arbeloa, Ignacio
    Amayuelas SL, Spain.
    Järn, Mikael
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Lundgren, Urban
    RISE - Research Institutes of Sweden, Säkerhet och transport, Elektronik.
    Welinder, Jan
    RISE - Research Institutes of Sweden, Säkerhet och transport, Elektronik.
    Johansson, Kenth
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Modelling anti-icing of railway overhead catenary wires by resistive heating2019Inngår i: International Journal of Heat and Mass Transfer, ISSN 0017-9310, E-ISSN 1879-2189, Vol. 143, artikkel-id 118505Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Aggregation of ice on electrical cables and apparatus can cause severe equipment malfunction and is thus considered as a serious problem, especially in arctic climate zones. In particular, cable damage caused by ice accumulation on railway catenary wires is in wintertime a common origin for delayed trains in the northern parts of Europe. This study examines how resistive heating can be used for preventing formation of ice on metallic, non-insulated electrical cables. The heat equation and the Navier Stokes equations were solved simultaneously with FEM in 3D in order to predict the cable temperature as function of external temperature, applied voltage, wind speed, wind direction, and heating time. An analytical expression for the heat transfer coefficient was derived from the FEM simulations and it was concluded that the influence of wind direction can typically be neglected. Experimental validation measurements were performed on Kanthal cables in a climate chamber, giving temperature increase results in good agreement with the simulation predictions. The resistive heating efficiency, i.e. the ratio between applied electrical energy and resulting thermal energy, was found to be approximately 68% in this particular study.

  • 117.
    Nuzzo, Marine
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. Lund University, Sweden.
    Sloth Overgaard, Jakob
    GEA Process Engineering AS, Denmark.
    Bergenståhl, Bjorn
    Lund University, Sweden.
    Millqvist-Fureby, Anna
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    The morphology and internal composition of dried particles from whole milk—From single droplet to full scale drying2017Inngår i: Food Structure, ISSN 2213-3291, Vol. 13, s. 35-44Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Powder structure and functionality are expected to be closely linked to the composition and drying process. In order to understand the optimization of the quality of a powder, e.g. encapsulation efficiency, or wetting and dispersion properties, monitoring of the particle microstructure is an attractive concept. However, to study the impact of different parameters in formulation and drying process on full scale is complicated and expensive, hence, studies on smaller scale, even single particle drying, is a potentially useful complement, as long as the results are comparable. The aim of this study is to compare morphology and internal composition of whole milk particles produced at different dryer scales to assess the development of internal structure in powder formed by spray drying. Whole milk was spray dried in the single particle dryer, laboratory dryer, pilot plant dryer and full scale dryer. The morphology and composition of the particles obtained were analyzed by low vacuum-SEM, confocal Raman microscopy and X-ray photoelectron spectroscopy. Phenomena such as adsorption of surface active compounds at the particle surface and phase segregation are observed to different extent, depending on particle size and drying time. The scale of drying influences the internal microstructure and distribution of components in the particles, and to a small extent also the external morphology. These effects are proposed to be related to the drying times for different droplet sizes, although mechanical handling effects and agglomeration in the full scale dryer may also influence the final morphology of these particles, as well as the surface composition.

  • 118.
    Oorni, Katariina
    et al.
    Wihuri Research Institute, Finland.
    Lehti, Satu
    Wihuri Research Institute, Finland.
    Sjövall, Peter
    RISE - Research Institutes of Sweden (2017-2019), Biovetenskap och material, Kemi och material.
    Kovanen, Petri T.
    Wihuri Research Institute, Finland.
    Triglyceride-Rich Lipoproteins as a Source of Proinflammatory Lipids in the Arterial Wall2019Inngår i: Current Medicinal Chemistry, ISSN 0929-8673, E-ISSN 1875-533X, Vol. 26, nr 9, s. 1701-1710Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Apolipoprotein B -containing lipoproteins include triglyceride-rich lipoproteins (chylomicrons and their remnants, and very low-density lipoproteins and their remnants) and cholesterol-rich low-density lipoprotein particles. Of these, lipoproteins having sizes below 70-80 nm may enter the arterial wall, where they accumulate and induce the formation of atherosclerotic lesions. The processes that lead to accumulation of lipoprotein-derived lipids in the arterial wall have been largely studied with a focus on the low-density lipoprotein particles. However, recent observational and genetic studies have discovered that the triglyceriderich lipoproteins and their remnants are linked with cardiovascular disease risk. In this review, we describe the potential mechanisms by which the triglyceride-rich remnant lipoproteins can contribute to the development of atherosclerotic lesions, and highlight the differences in the atherogenicity between low-density lipoproteins and the remnant lipoproteins.[on SciFinder (R)]

  • 119.
    Pansch, Christian
    et al.
    GEOMAR Helmholtz Centre for Ocean Research Kiel, Germany; University of Gothenburg, Sweden.
    Jonsson, Per R.
    University of Gothenburg, Sweden.
    Berglin, Mattias
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Pinori, Emiliano
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Wrange, Anna-Lisa
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    A new flow-through bioassay for testing low-emission antifouling coatings2017Inngår i: Biofouling (Print), ISSN 0892-7014, E-ISSN 1029-2454, Vol. 33, nr 8, s. 613-623Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Current antifouling (AF) technologies are based on the continuous release of biocides into the water, and consequently discharge into the environment. Major efforts to develop more environmentally friendly coatings require efficient testing in laboratory assays, followed by field studies. Barnacles are important fouling organisms worldwide, increasing hydrodynamic drag on ships and damaging coatings on underwater surfaces, and thus are extensively used as models in AF research, mostly in static, laboratory-based systems. Reliable flow-through test assays for the screening of biocide-containing AF paints, however, are rare. Herein, a flow-through bioassay was developed to screen for diverse low-release biocide paints, and to evaluate their effects on pre- and post-settlement traits in barnacles. The assay distinguishes between the effects from direct surface contact and bulk-water effects, which are crucial when developing low-emission AF coatings. This flow-through bioassay adds a new tool for rapid laboratory-based first-stage screening of candidate compounds and novel AF formulations.

  • 120.
    Pedersen, Emma
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Fant, Kristina
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Guidance Document on Good In Vitro Method Practices (GIVIMP): Series on Testing and Assessment No. 2862018Annet (Annet vitenskapelig)
  • 121.
    Persson, Karin
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Ahniyaz, Anwar
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Magnér, Jörgen
    IVL Swedish Environmental Research Institute, Sweden.
    Royen, Hugo
    IVL Swedish Environmental Research Institute, Sweden.
    Filipsson, Staffan
    IVL Swedish Environmental Research Institute, Sweden.
    A Photocatalytic Membrane: for Treatment of Pharmaceuticals in Wastewater2017Rapport (Annet vitenskapelig)
    Abstract [en]

    Toxic organics, pharmaceuticals and antibiotics are currently only partially or not at all removed from wastewater, as today’s wastewater treatment will only partly degrade those substances. Therefore, those substances will be found in the effluent from wastewater treatment plants and this can be a threat to both human health and aquatic species.

    Photocatalytic membranes show great promise as a method to combat the challenge of toxic organics in wastewater. The novel photocatalytic membrane developed in the project was shown to photocatalytically decompose organic compounds such as pharmaceutical residues and dyes in both tap water and treated effluent from a membrane bioreactor (MBR) wastewater treatment process. Several parameters affecting the affinity of the pharmaceuticals to the membrane surface, such as the hydrophobicity and pKa of the pharmaceuticals and the pH of the water, were shown to affect the efficacy of the removal.

    Finally, when irradiated with UV light the photocatalytic membrane showed promise of keeping high flux and reducing downtime by lengthening the cleaning cycle.

    Fulltekst (pdf)
    fulltext
  • 122.
    Petersen, Henrik
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. Chalmers University of Technology, Sweden.
    Jakubowicz, Ignacy
    RISE - Research Institutes of Sweden, Samhällsbyggnad, Energi och cirkulär ekonomi.
    Enebro, Jonas
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Yarahmadi, Nazdaneh
    RISE - Research Institutes of Sweden, Samhällsbyggnad, Energi och cirkulär ekonomi.
    Solid-state organo-modification of montmorillonite for manufacturing of plasticized poly(vinyl chloride) nanocomposites2018Inngår i: Journal of Vinyl and Additive Technology, ISSN 1083-5601, Vol. 24, s. E146-E153Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The preparation of poly(vinyl chloride) (PVC) nanocomposites via direct melt processing is still posing problems mainly because of the lack of availability of suitable commercial organoclays and because of the low thermal stability of PVC. A new type of organic compounds for modifying montmorillonite (MMT), which is suitable for use in plasticized PVC, has been found earlier. The current study shows that it is possible to achieve partially exfoliated PVC nanocomposites with greatly improved mechanical properties using a method of liquid-solid–state intercalation of MMT when using tributyl citrate and diisononyl phthalate (DINP) plasticizers as organic modifiers. It is also shown that liquid mixed metal stabilizers have the ability to intercalate the clay at least when DINP is used. The observation raises questions regarding how this phenomenon can affect the thermal stability of PVC composites. J. VINYL ADDIT. TECHNOL., 24:E146–E153, 2018. © 2017 Society of Plastics Engineers.

  • 123.
    Pettersson, Jens
    et al.
    Linnaeus University, Sweden.
    Andersson, Sven
    Babcock & Wilcox Vølund AB, Sweden; Chalmers University of Technology, Sweden.
    Bäfver, Linda
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Strand, Michael
    Linnaeus University, Sweden.
    Investigation of the Collection Efficiency of a Wet Electrostatic Precipitator at a Municipal Solid Waste-Fueled Combined Heat and Power Plant Using Various Measuring Methods2019Inngår i: Energy & Fuels, ISSN 0887-0624, E-ISSN 1520-5029, Vol. 33, nr 6, s. 5282-5292Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    This article reports results from measurements of mainly submicrometer particles at the inlet and outlet of a newly designed industrial wet electrostatic precipitator (WESP) in a combined heat and power plant fueled with municipal solid waste. The measurements were carried out with dual electric low-pressure impactors in parallel at the precipitator inlet and outlet. In addition, measurements were carried out with traditional total dust filters, low-pressure impactors, a scanning mobility particle sizer, and an aerodynamic particle sizer. The measurements aimed to characterize the aerosol particles and measure the efficiency of the WESP with special attention to fine and ultrafine particles. In general, the WESP performance and response to varying conditions was found to be in line with predictions made for the design. The WESP featured a cooled collector surface, but based on the limited results, no conclusion could be drawn regarding any possible improvement from collector cooling. The characterization of the aerosol particulate matter was challenging because of fast fluctuations in particle concentration. Methodological considerations are pointed out, mainly regarding the SMPS and ELPI measuring systems.

  • 124.
    Pilkington, Georgia A.
    et al.
    KTH Royal Institute of Technology, Sweden.
    Harris, Kathryn
    KTH Royal Institute of Technology, Sweden.
    Bergendal, Erik
    KTH Royal Institute of Technology, Sweden.
    Reddy, Akepati Bhaskar
    KTH Royal Institute of Technology, Sweden.
    Palsson, Gunnar K.
    Uppsala University, Sweden.
    Vorobiev, Alexei
    Uppsala University, Sweden.
    Antzutkin, Oleg N.
    Luleå University of Technology, Sweden.
    Glavatskih, Sergei
    KTH Royal Institute of Technology, Sweden ; Ghent University, Belgium.
    Rutland, Mark W.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. KTH Royal Institute of Technology, Sweden.
    Electro-responsivity of ionic liquid boundary layers in a polar solvent revealed by neutron reflectance2018Inngår i: Journal of Chemical Physics, ISSN 0021-9606, E-ISSN 1089-7690, Vol. 148, nr 19, artikkel-id 193806Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Using neutron reflectivity, the electro-responsive structuring of the non-halogenated ionic liquid (IL) trihexyl(tetradecyl)phosphonium-bis(mandelato)borate, [P6,6,6,14][BMB], has been studied at a gold electrode surface in a polar solvent. For a 20% w/w IL mixture, contrast matched to the gold surface, distinct Kiessig fringes were observed for all potentials studied, indicative of a boundary layer of different composition to that of the bulk IL-solvent mixture. With applied potential, the amplitudes of the fringes from the gold-boundary layer interface varied systematically. These changes are attributable to the differing ratios of cations and anions in the boundary layer, leading to a greater or diminished contrast with the gold electrode, depending on the individual ion scattering length densities. Such electro-responsive changes were also evident in the reflectivities measured for the pure IL and a less concentrated (5% w/w) IL-solvent mixture at the same applied potentials, but gave rise to less pronounced changes. These measurements, therefore, demonstrate the enhanced sensitivity achieved by contrast matching the bulk solution and that the structure of the IL boundary layers formed in mixtures is strongly influenced by the bulk concentration. Together these results represent an important step in characterising IL boundary layers in IL-solvent mixtures and provide clear evidence of electro-responsive structuring of IL ions in their solutions with applied potential.

  • 125.
    Raj, Akanksha
    et al.
    KTH Royal Institute of Technology, Sweden.
    Wang, Min
    KTH Royal Institute of Technology, Sweden.
    Liu, Chao
    KTH Royal Institute of Technology, Sweden.
    Ali, Liaquat
    University of Gothenburg, Sweden.
    Karlsson, Niclas G.
    University of Gothenburg, Sweden.
    Claesson, Per M.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi, material och ytor. KTH Royal Institute of Technology, Sweden.
    Dėdinaitė, Andra
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi, material och ytor. KTH Royal Institute of Technology, Sweden.
    Molecular synergy in biolubrication: The role of cartilage oligomeric matrix protein (COMP) in surface-structuring of lubricin2017Inngår i: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 495, s. 200-206Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Hypothesis Synovial surfaces are lubricated by biomolecular aggregates that act in synergy, and lubricin is one key biolubricant. Its molecular structure allows extensive hydration and this is conducive to its lubrication performance. However, in order to fullfil its lubrication function it needs to be anchored and oriented on the surface in a proper way. We suggest that cartilage oligomeric matrix protein (COMP) is one of the biomolecules that promotes anchoring of lubricin in a fashion that facilitates lubrication. Experiments Weakly hydrophobic poly(methyl methacrylate) (PMMA) surfaces were coated by COMP and lubricin, individually and in combinations. Adsorption was investigated using a quartz crystal microbalance, and friction between the biopolymer-coated surfaces was determined by employing the atomic force microscope-colloidal probe technique. Findings It was found that COMP facilitated firm directed attachment of lubricin in a manner that resulted in low friction forces, significantly lower than what was achieved when lubricin was directly adsorbed to PMMA. Evidently, COMP provides means for lubricin to attach strongly and in a favourable conformation for efficient lubrication of this surface. We suggest that our findings can be extrapolated to cartilage surfaces, where co-localization of COMP and lubricin has been demonstrated.

  • 126.
    Raj, Akanksha
    et al.
    KTH Royal Institute of Technology, Sweden.
    Wang, Min
    KTH Royal Institute of Technology, Sweden.
    Zander, Thomas
    Institute for Materials Research, Germany.
    Wieland, D. C. Florian
    Institute for Materials Research, Germany.
    Liu, Xiaoyan
    KTH Royal Institute of Technology, Sweden.
    An, Junxue
    KTH Royal Institute of Technology, Sweden.
    Garamus, Vasil M.
    Institute for Materials Research, Germany.
    Willumeit-Römer, Regine
    Institute for Materials Research, Germany.
    Fielden, Matthew
    KTH Royal Institute of Technology, Sweden.
    Claesson, Per M.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi, material och ytor. KTH Royal Institute of Technology, Sweden.
    Dėdinaitė, Andra
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi, material och ytor. KTH Royal Institute of Technology, Sweden.
    Lubrication synergy: Mixture of hyaluronan and dipalmitoylphosphatidylcholine (DPPC) vesicles2017Inngår i: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 488, s. 225-233Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Phospholipids and hyaluronan have been implied to fulfil important roles in synovial joint lubrication. Since both components are present in synovial fluid, self-assembly structures formed by them should also be present. We demonstrate by small angle X-ray scattering that hyaluronan associates with the outer shell of dipalmitoylphophatidylcholine (DPPC) vesicles in bulk solution. Further, we follow adsorption to silica from mixed hyaluronan/DPPC vesicle solution by Quartz Crystal Microbalance with Dissipation measurements. Atomic Force Microscope imaging visualises the adsorbed layer structure consisting of non-homogeneous phospholipid bilayer with hyaluronan/DPPC aggregates on top. The presence of these aggregates generates a long-range repulsive surface force as two such surfaces are brought together. However, the aggregates are easily deformed, partly rearranged into multilayer structures and partly removed from between the surfaces under high loads. These layers offer very low friction coefficient (<0.01), high load bearing capacity (≈23 MPa), and self-healing ability. Surface bound DPPC/hyaluronan aggregates provide a means for accumulation of lubricating DPPC molecules on sliding surfaces.

  • 127.
    Reeve, P. A. P.
    et al.
    University of Sheffield, Uk.
    Grabowska, U.
    Medivir AB, Sweden; F-star Biotechnology Ltd, UK.
    Oden, L. S.
    Medivir AB, Sweden.
    Wiktelius, D.
    Medivir AB, Sweden; FOI Swedish Defence Research Agency, Sweden.
    Wångsell, Fredrik
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. Medivir AB, Sweden.
    Jackson, R. F. W.
    University of Sheffield, Uk.
    Radical Functionalization of Unsaturated Amino Acids: Synthesis of Side-Chain-Fluorinated, Azido-Substituted, and Hydroxylated Amino Acids2019Inngår i: ACS Omega, ISSN 2470-1343, Vol. 4, nr 6, s. 10854-10865Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    A range of enantiomerically pure protected side-chain-fluorinated amino acids has been prepared (13 examples) by treatment of protected amino acids containing unsaturated side chains with a combination of Fe(III)/NaBH4 and Selectfluor. The modification of the conditions by replacement of Selectfluor with NaN3 allowed the preparation of side-chain azido-substituted amino acids (five examples), which upon catalytic hydrogenation gave the corresponding amines, isolated as lactams (four examples). Radical hydration of the unsaturated side chains leading to side-chain-hydroxylated protected amino acids has also been demonstrated.

  • 128.
    Rissler, Jenny
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. Lund University, Sweden.
    Gudmundsson, Anders
    Lund University, Sweden.
    Nicklasson, Hanna
    SUS, Sweden.
    Swietlicki, Erik
    Lund University, Sweden.
    Wollmer, Per
    SUS, Sweden.
    Löndahl, Jacob
    Lund University, Sweden.
    Deposition efficiency of inhaled particles (15-5000 nm) related to breathing pattern and lung function: An experimental study in healthy children and adults2017Inngår i: Particle and Fibre Toxicology, ISSN 1743-8977, E-ISSN 1743-8977, Vol. 14, nr 1, artikkel-id 10Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background: Exposure to airborne particles has a major impact on global health. The probability of these particles to deposit in the respiratory tract during breathing is essential for their toxic effects. Observations have shown that there is a substantial variability in deposition between subjects, not only due to respiratory diseases, but also among individuals with healthy lungs. The factors determining this variability are, however, not fully understood. Method: In this study we experimentally investigate factors that determine individual differences in the respiratory tract depositions of inhaled particles for healthy subjects at relaxed breathing. The study covers particles of diameters 15-5000 nm and includes 67 subjects aged 7-70 years. A comprehensive examination of lung function was performed for all subjects. Principal component analyses and multiple regression analyses were used to explore the relationships between subject characteristics and particle deposition. Results: A large individual variability in respiratory tract deposition efficiency was found. Individuals with high deposition of a certain particle size generally had high deposition for all particles <3500 nm. The individual variability was explained by two factors: breathing pattern, and lung structural and functional properties. The most important predictors were found to be breathing frequency and anatomical airway dead space. We also present a linear regression model describing the deposition based on four variables: tidal volume, breathing frequency, anatomical dead space and resistance of the respiratory system (the latter measured with impulse oscillometry). Conclusions: To understand why some individuals are more susceptible to airborne particles we must understand, and take into account, the individual variability in the probability of particles to deposit in the respiratory tract by considering not only breathing patterns but also adequate measures of relevant structural and functional properties.

  • 129.
    Rissler, Jenny
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. Lund University, Sweden .
    Nicklasson, Hanna
    SUS, Sweden.
    Gudmundsson, Anders
    Lund University, Sweden .
    Wollmer, Per
    SUS, Sweden.
    Swietlicki, Erik
    Lund University, Sweden .
    Löndahl, Jakob
    Lund University, Sweden .
    A set-up for respiratory tract deposition efficiency measurements (15–5000 nm) and first results for a group of children and adults2017Inngår i: Aerosol and Air Quality Research, ISSN 1680-8584, E-ISSN 2071-1409, Vol. 17, nr 6, s. 1244-1255Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Exposure to airborne particulate matter is associated with a number of negative health effects ranging from respiratorydiseases to systemic effects and cancer. One important factor for understanding the health effects is the individual variationin the respiratory tract deposition of inhaled particles. In this study, we describe an experimental set-up for size-resolvedmeasurements of the lung deposited fraction of airborne particles, covering the diameter range from 15 to 5000 nm. Theset-up includes a system for generating a stable aerosol with a sufficiently broad size distribution. We used a scanningmobility particle sizer and an aerodynamic particle sizer to determine particle number and size. The set-up was used toinvestigate individual differences in the deposition fraction (DF) of particles in the respiratory tract for a group of 67subjects of both sexes aged 7–70 years. The measured DF was applied to two model aerosols, one representing an urbanenvironment and one a rural environment, and the particle deposition rates were derived (i.e., the deposited amount ofparticles per unit time). Furthermore, the deposition rates were normalized to lung surface area and body mass – two dosemeasures that are considered relevant for the health effects of airborne particles. In addition to validation of the set-up, weshow that there is a large individual variation in DF, with some subjects having a DF that is more than twice as high as thatof others. Although we observe differences in the DF between different subgroups, most individual variation wasexplained neither by age nor by gender. When normalizing the deposition rates to lung surface area or body mass, thedeposition rates of children become significantly higher than those of adults. Furthermore, the individual variability islarger for the lung surface area or body mass normalized deposition rates than for DF

  • 130.
    Rouhi, Mohammad
    et al.
    RISE - Research Institutes of Sweden, Material och produktion, SICOMP.
    Juntikka, Magdalena
    RISE - Research Institutes of Sweden, Material och produktion, SICOMP.
    Landberg, Johan
    RISE - Research Institutes of Sweden, Material och produktion, IVF.
    Wysocki, Maciej
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Assessing models for the prediction of mechanical properties for the recycled short fibre composites2019Inngår i: Journal of reinforced plastics and composites (Print), ISSN 0731-6844, E-ISSN 1530-7964, Vol. 38, nr 10, s. 454-466Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Processing of polymer fibre composites has a remarkable influence on their mechanical performance. These mechanical properties are even more influenced when using recycled reinforcement. Therefore, we place particular attention on the evaluation of micromechanical models to estimate the mechanical properties and compare them against the experimental results of the manufactured composites from recycled carbon fibre material. For the manufacturing process, an epoxy matrix and carbon fibre production cut-offs as reinforcing material are incorporated using a vacuum infusion process. In addition, continuous textile reinforcement in combination with the epoxy matrix is used as reference material to evaluate the degradation of mechanical performance of the recycled composite. The experimental results show higher degradation of the composite strength compared to the stiffness properties. Observations from the modelling also show the same trend as the deviation between the theoretical and experimental results is lower for stiffness comparisons than the strength calculations. Yet still, good mechanical performance for specific applications can be expected from these materials.

  • 131.
    Rouhi, Mohammad
    et al.
    RISE - Research Institutes of Sweden, Material och produktion, SICOMP.
    Wysocki, Maciej
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    SIMULATION OF 3D PREPREG CONSOLIDATIONPROCESS USING SOLID SHELL ELEMENTS2018Konferansepaper (Annet vitenskapelig)
    Abstract [en]

    In process simulation of composite materials, 3D simulation of manufacturing processes is desirableconsidering the manufacturing trend where parts became more complex leading to complex3D stress-strain states. Moreover, coupling of sub-processes that are happening simultaneouslysuch as macro-scale preform processes, micro-infiltration and solid and fluid interactionrequires full 3D description of the problem.The development is exemplified considering compression moulding process of prepregs wherethe main focus of the modeling will be on the compression and compaction of directionalprepreg laminate and flow consolidation. To this end, the theory of two phase porous mediais used along with assuming hyper-elastic material response for the laminate to formulatethe problem. A finite element formulation and implementation of the two-phase problem is developedfor incompressible constituents and is implemented in a user defined element (UEL)to be used with Abaqus.

  • 132.
    Rozenbaum, Rene
    et al.
    University of Groningen, Netherlands.
    Su, Linzhu
    University of Groningen, Netherlands; Nankai University, China.
    Umerska, Anita
    Université Bretagne, France.
    Eveillard, Matthiou
    Université de Nantes, France.
    Håkansson, Joakim
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Mahlapuu, Margit
    Promore Pharma, Sweden.
    Huang, Fan
    Peking Union Medical College, China.
    Liu, Jian feng
    Peking Union Medical College, China.
    Zhang, Zhenkun
    Nankai University, China.
    Shi, Linqi
    Nankai University, China.
    van der Mei, Henny
    University of Groningen, Netherlands.
    Busscher, Henk
    University of Groningen, Netherlands.
    Sharma, Prashant
    University of Groningen, Netherlands.
    Antimicrobial synergy of monolaurin lipid nanocapsules with adsorbed antimicrobial peptides against Staphylococcus aureus biofilms in vitro is absent in vivo2019Inngår i: Journal of Controlled Release, ISSN 0168-3659, E-ISSN 1873-4995, Vol. 293, s. 73-83Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Bacterial infections are mostly due to bacteria in their biofilm-mode of growth, while penetrability of antimicrobials into infectious biofilms and increasing antibiotic resistance hamper infection treatment. In-vitro, monolaurin lipid nanocapsules (ML-LNCs) carrying adsorbed antimicrobial peptides (AMPs) displayed synergistic efficacy against planktonic Staphylococcus aureus, but it has not been demonstrated, neither in-vitro nor in-vivo, that such ML-LNCs penetrate into infectious S. aureus biofilms and maintain synergy with AMPs. This study investigates the release mechanism of AMPs from ML-LNCs and possible antimicrobial synergy of ML-LNCs with the AMPs DPK-060 and LL-37 against S. aureus biofilms in-vitro and in a therapeutic, murine, infected wound-healing model. Zeta potentials demonstrated that AMP release from ML-LNCs was controlled by the AMP concentration in suspension. Both AMPs demonstrated no antimicrobial efficacy against four staphylococcal strains in a planktonic mode, while a checkerboard assay showed synergistic antimicrobial efficacy when ML-LNCs and DPK-060 were combined, but not for combinations of ML-LNCs and LL-37. Similar effects were seen for growth reduction of staphylococcal biofilms, with antimicrobial synergy persisting only for ML-LNCs at the highest level of DPK-060 or LL-37 adsorption. Healing of wounds infected with bioluminescent S. aureus Xen36, treated with ML-LNCs alone, was faster when treated with PBS, while AMPs alone did not yield faster wound-healing than PBS. Faster, synergistic wound-healing due to ML-LNCs with adsorbed DPK-060, was absent in-vivo. Summarizing, antimicrobial synergy of ML-LNCs with adsorbed antimicrobial peptides as seen in-vitro, is absent in in-vivo healing of infected wounds, likely because host AMPs adapted the synergistic role of the AMPs added. Thus, conclusions regarding synergistic antimicrobial efficacy, should not be drawn from planktonic data, while even in-vitro biofilm data bear little relevance for the in-vivo situation. © 2018

  • 133.
    Rutland, Mark W.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. KTH Royal Institute of Technology, Sweden.
    Chemical physics of electroactive materials: Concluding remarks2017Inngår i: Faraday discussions (Online), ISSN 1359-6640, E-ISSN 1364-5498, Vol. 199, s. 615-630Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    It is an honour to be charged with providing the concluding remarks for a Faraday Discussion. As many have remarked before, it is nonetheless a prodigious task, and what follows is necessarily a personal, and probably perverse, view of a watershed event in the Chemical Physics of Electroactive materials. The spirit of the conference was captured in a single sentence during the meeting itself. "It is the nexus between rheology, electrochemistry, colloid science and energy storage". The current scientific climate is increasingly dominated by a limited number of global challenges, and there is thus a tendency for research to resemble a football match played by 6 year olds, where everyone on the field chases the (funding) ball instead of playing to their "discipline". It is thus reassuring to see how the application of rigorous chemical physics is leading to ingenious new solutions for both energy storage and harvesting, via, for example, nanoactuation, electrowetting, ionic materials and nanoplasmonics. In fact, the same language of chemical physics allows seamless transition between applications as diverse as mechano-electric energy generation, active moisture transport and plasmonic shutters-even the origins of life were addressed in the context of electro-autocatalysis!.

  • 134.
    Schweitzer, Mary
    et al.
    North Carolina State University, USA; North Carolina Museum of Natural Sciences, USA; Lund University, Sweden.
    Zheng, Wenxia
    North Carolina State University, USA.
    Moyer, Alison
    Drexel University, USA.
    Sjövall, Peter
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Lindgren, Johan
    Lund University, Sweden.
    Preservation potential of keratin in deep time2018Inngår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 13, nr 11Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Multiple fossil discoveries and taphonomic experiments have established the durability of keratin. The utility and specificity of antibodies to identify keratin peptides has also been established, both in extant feathers under varying treatment conditions, and in feathers from extinct organisms. Here, we show localization of feather-keratin antibodies to control and heat-treated feathers, testifying to the repeatability of initial data supporting the preservation potential of keratin. We then show new data at higher resolution that demonstrates the specific response of these antibodies to the feather matrix, we support the presence of protein in heat-treated feathers using ToF-SIMS, and we apply these methods to a fossil feather preserved in the unusual environment of sinter hot springs. We stress the importance of employing realistic conditions such as sediment burial when designing experiments intended as proxies for taphonomic processes occurring in the fossil record. Our data support the hypothesis that keratin, particularly the β-keratin that comprises feathers, has potential to preserve in fossil remains.

  • 135.
    Siljeström, Sandra
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Parenteau, Mary
    Carnegie Institution of Washington, USA.
    Jahnke, Linda
    NASA Ames Research Center, USA.
    Cady, Sherry
    Pacific Northwest National Laboratory, USA.
    A comparative ToF-SIMS and GC–MS analysis of phototrophic communities collected from an alkaline silica-depositing hotspring2017Inngår i: Organic Geochemistry, ISSN 0146-6380, E-ISSN 1873-5290, Vol. 109, s. 14-30Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    One of few techniques that is able to spatially resolve chemical data, including organic molecules, to morphologicalfeatures in modern and ancient geological samples, is time-of-flight secondary ion mass spectrometry(ToF-SIMS). The ability to connect chemical data to morphology is key for interpreting thebiogenicity of preserved remains in ancient samples. However, due to the lack of reference data for geologicallyrelevant samples and the ease with which samples can be contaminated, ToF-SIMS data may bedifficult to interpret. In this project, we aimed to build a ToF-SIMS spectral database by performing parallelToF-SIMS and gas chromatography–mass spectrometry (GC–MS) analyses of extant photosyntheticmicrobial communities collected from an alkaline silica-depositing hot spring in Yellowstone NationalPark, USA. We built the library by analyzing samples of increasing complexity: pure lipid standards commonlyfound in thermophilic phototrophs, solvent extracts of specific lipid fractions, total lipid extracts,pure cultures of dominant phototrophic community members, and unsilicified phototrophic streamercommunities.The results showed that important lipids and pigments originating from phototrophs were detected byToF-SIMS (e.g., wax esters, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, sufloquinovosyldiaglycerol,alkanes, etc.) in the streamer lipid extracts. Many of the lipids were also detected in situin the unsilicified streamer, and could even be spatially resolved to individual cells within the streamercommunity. Together with the ToF-SIMS database, this mapping ability will be used to further exploreother microbial mats and their fossilized counterparts in the geological record. This is likely to expandthe geochemical understanding of these types of samples.

    Fulltekst (pdf)
    fulltext
  • 136.
    Siljeström, Sandra
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Thiel, V.
    University of Göttingen, Germany.
    Steele, A.
    Carnegie Institution of Science, US.
    Preservation of heme derivatives in vertebrate fossils from the Messel Pit and Enspel, Germany2019Inngår i: 29th International Meeting on Organic Geochemistry, IMOG 2019, European Association of Geoscientists and Engineers, EAGE , 2019Konferansepaper (Fagfellevurdert)
  • 137.
    Sjövall, Peter
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Pomerantz, Andrew E.
    Schlumberger-Doll Research, USA.
    Lu, Xiaohu
    Nynäs AB, Sweden.
    Mullins, Oliver C.
    Schlumberger-Doll Research, USA.
    Time of flight-secondary ion mass spectrometry (TOF-SIMS) study of diverse asphaltenes2018Inngår i: Fuel, ISSN 0016-2361, E-ISSN 1873-7153, Vol. 220, s. 638-644Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Mass spectrometry has been used to elucidate a large variety of properties of asphaltenes. Here, time-of-flight secondary ion mass spectrometry (TOF-SIMS) is used to probe three diverse asphaltene types with wide ranging fractions of alkyl carbon to the sum of alkyl plus aromatic carbon (Rc), immature source rock asphaltenes (ISA with Rc ∼ 0.75); petroleum asphaltenes (PA with Rc ∼ 0.5); and coal-derived asphaltenes (CDA with Rc ∼ 0.25). In addition, the asphaltenes from a commercial bitumen are examined. Primary ion surface bombardment using the Bi3 + ion yields high energy density of deposition and significant molecular fragmentation with secondary ion formation. Formation of free radical cation fragments is generally suppressed especially for smaller fragments except for specific cases. Possible structures, especially cations of common aromatic compounds, are suggested for fragments with relatively large cross section of formation. Principal component analysis of the fragmentograms allows identification of key properties of the complex fragmentation patterns for the different samples. Comparisons of TOF-SIMS fragmentograms show a fundamental difference for small fragments between CDAs and all petroleum derived asphaltenes with CDAs being dominated by aromatic carbon fragments whereas all petroleum derived asphaltenes show a large fraction of fragments from alkyl carbon. However, the type of alkyl carbon fragments did not exhibit systematic trends with Rc, nor with the extent of chemical reaction or chemical processing of the materials. Consequently, it appears that having an appreciable alkane fraction is a basic property of petroleum derived asphaltenes, but the exact type of alkane carbon for differing samples of these asphaltenes is more idiosyncratic than systematic. 

  • 138.
    Sjövall, Peter
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Skedung, Lisa
    RISE - Research Institutes of Sweden, Biovetenskap och material, Yta, process och formulering.
    Gregoire, Sébastien
    L’OREAL Research and Innovation, France.
    Biganska, Olga
    L’OREAL Research and Innovation, France.
    Clément, Franck
    L’OREAL Research and Innovation, France.
    Luengo, Gustavo S
    L’OREAL Research and Innovation, France.
    Imaging the distribution of skin lipids and topically applied compounds in human skin using mass spectrometry2018Inngår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, nr 1, artikkel-id 16683Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The barrier functions of skin against water loss, microbial invasion and penetration of xenobiotics rely, in part, on the spatial distribution of the biomolecular constituents in the skin structure, particularly its horny layer (stratum corneum). However, all skin layers are important to describe normal and dysfunctional skin conditions, and to develop adapted therapies or skin care products. In this work, time-of-flight secondary ion mass spectrometry (ToF-SIMS) combined with scanning electron microscopy (SEM) was used to image the spatial distribution of a variety of molecular species, from stratum corneum down to dermis, in cross-section samples of human abdominal skin. The results demonstrate the expected localization of ceramide and saturated long-chain fatty acids in stratum corneum (SC) and cholesterol sulfate in the upper part of the viable epidermis. The localization of exogenous compounds is demonstrated by the detection and imaging of carvacrol (a constituent of oregano or thyme essential oil) and ceramide, after topical application onto ex vivo human skin. Carvacrol showed pronounced accumulation to triglyceride-containing structures in the deeper parts of dermis. In contrast, the exogenous ceramide was found to be localized in SC. Furthermore, the complementary character of this approach with classical ex vivo skin absorption analysis methods is demonstrated.

  • 139.
    Skedung, Lisa
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    El Rawadi, Charles
    L Oreal Research and Innovation, France.
    Arvidsson, Martin
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Farcet, Celine
    L Oreal Research and Innovation, France.
    Luengo, Gustavo S
    L Oreal Research and Innovation, France.
    Breton, Lionel
    L Oreal Research and Innovation, France.
    Rutland, Mark W.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. KTH Royal Institute of Technology, Sweden.
    Mechanisms of tactile sensory deterioration amongst the elderly2018Inngår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, nr 1, artikkel-id 5303Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    It is known that roughness-smoothness, hardness-softness, stickiness-slipperiness and warm-cold are predominant perceptual dimensions in macro-, micro- and nano- texture perception. However, it is not clear to what extent active tactile texture discrimination remains intact with age. The general decrease in tactile ability induces physical and emotional dysfunction in elderly, and has increasing significance for an aging population. We report a method to quantify tactile acuity based on blinded active exploration of systematically varying micro-textured surfaces and a same-different paradigm. It reveals that elderly participants show significantly reduced fine texture discrimination ability. The elderly group also displays statistically lower finger friction coefficient, moisture and elasticity, suggesting a link. However, a subpopulation of the elderly retains discrimination ability irrespective of cutaneous condition and this can be related to a higher density of somatosensory receptors on the finger pads. Skin tribology is thus not the primary reason for decline of tactile discrimination with age. The remediation of cutaneous properties through rehydration, however leads to a significantly improved tactile acuity. This indicates unambiguously that neurological tactile loss can be temporarily compensated by restoring the cutaneous contact mechanics. Such mechanical restoration of tactile ability has the potential to increase the quality of life in elderly. 

    Fulltekst (pdf)
    fulltext
  • 140.
    Skoglund, Sara
    et al.
    KTH Royal Institute of Technology, Sweden.
    Blomberg, Eva
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi, material och ytor. KTH Royal Institute of Technology, Sweden.
    Wallinder, Inger O.
    KTH Royal Institute of Technology, Sweden.
    Grillo, Isabelle
    Institut Laue Langevin, France.
    Pedersen, Jan S.
    Aarhus University, Denmark.
    Bergström, L Magnus
    KTH Royal Institute of Technology, Sweden.
    A novel explanation for the enhanced colloidal stability of silver nanoparticles in the presence of an oppositely charged surfactant2017Inngår i: Physical Chemistry, Chemical Physics - PCCP, ISSN 1463-9076, E-ISSN 1463-9084, Vol. 19, nr 41, s. 28037-28043Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The structural behavior in aqueous mixtures of negatively charged silver nanoparticles (Ag NPs) together with the cationic surfactants cetyltrimethylammonium bromide (CTAB) and dodecyltrimethylammonium chloride (DTAC), respectively, has been investigated using SANS and SAXS. From our SANS data analysis we are able to conclude that the surfactants self-assemble into micellar clusters surrounding the Ag NPs. We are able to quantify our results by means of fitting experimental SANS data with a model based on cluster formation of micelles with very good agreement. Based on our experimental results, we propose a novel mechanism for the stabilization of negatively charged Ag NPs in a solution of positively charged surfactants in which cluster formation of micelles in the vicinity of the particles prevents the particles from aggregating. Complementary SAXS and DLS measurements further support this novel way of explaining stabilization of small hydrophilic nanoparticles in surfactant-containing solutions.

  • 141.
    Skoglund, Sara
    et al.
    KTH Royal Institute of Technology, Sweden.
    Hedberg, Jonas
    KTH Royal Institute of Technology, Sweden.
    Yunda, Elena
    KTH Royal Institute of Technology, Sweden; National Research Tomsk Polytechnic University, Russia.
    Godymchuk, Anna
    National Research Tomsk Polytechnic University, Russia; National University of Science and Technology, Russia.
    Blomberg, Eva
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. KTH Royal Institute of Technology, Sweden.
    Odnevall Wallinder, Inger
    KTH Royal Institute of Technology, Sweden.
    Difficulties and flaws in performing accurate determinations of zeta potentials of metal nanoparticles in complex solutions - Four case studies2017Inngår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, nr 7, artikkel-id e0181735Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The zeta potential (ZP) is a parameter commonly used to characterize metal nanoparticles (NPs) in solution. Such determinations are for example performed in nanotoxicology since the ZP influences e.g. the interaction between cells and different biomolecules. Four case studies on different metal NPs (Cu and Zn NPs, and citrate capped Ag NPs) are presented in this study in order to provide guidance on how to accurately interpret and report ZP data. Solutions of high ionic strength (150 mM NaCl) induce a higher extent of particle agglomeration (elucidated with Ag NPs) when compared with conditions in 10 mM NaCl, which further complicates the prediction of the ZP due to e.g. sedimentation and broadening of the zeta potential distribution. The particle size is seldom included specifically in the standard ways of determining ZP (Hückel and Smoluchowski approximations). However corrections are possible when considering approximations of the Henry function. This was seen to improve the analysis of NPs, since there are cases when both the Hückel and the Smulochowski approximations are invalid. In biomolecule-containing cell media (BEGM), the signal from e. g. proteins may interfere with the measured ZP of the NPs. The intensity distribution of the ZP of both the blank solution and the solution containing NPs should hence be presented in addition to the mean value. Due to an increased ionic strength for dissolving of metal NPs (exemplified by Zn NPs), the released metal ions must be considered when interpreting the zeta potential measurements. In this work the effect was however negligible, as the particle size was several hundred nm, conditions that made the Smoluchowski approximation valid despite an increased ionic strength. However, at low ionic strengths (mM range) and small-sized NPs (tens of nm), the effect of released metal ions can influence the choice of model for determining the zeta potential. Sonication of particle dispersions influences not only the extent of metal release but also the outermost surface oxide composition, which often results in an increased ZP. Surface compositional changes were illustrated for sonicated and non-sonicated Cu NPs. In all, it can be concluded that accurate measurements and interpretations are possible in most cases by collecting and reporting complementary data on characteristics such as particle size, ZP distributions, blank sample information, and particle oxide composition. © 2017, Public Library of Science. All rights reserved. This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

  • 142.
    Spezzati, Guilia
    et al.
    Eindhoven University of Technology, The Netherlands.
    Fant, Kristina
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Ahniyaz, Anwar
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Lundin Johnson, Maria
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Hensen, Emiel J. M.
    Eindhoven University of Technology, The Netherlands.
    Langermans, Harm
    DSM ChemTech Center, The Netherlands.
    Hofmann, Jan P.
    Eindhoven University of Technology, The Netherlands.
    Synthesis, Physicochemical Characterization, and Cytotoxicity Assessment of CeO2 Nanoparticles with Different Morphologies2017Inngår i: European Journal of Inorganic Chemistry, ISSN 1434-1948, E-ISSN 1099-1948, Vol. 2017, nr 25, s. 3184-3190Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    With the growing use of nanomaterials, it is essential to carefully determine whether they represent a risk for potential users. So far, validated stand-alone methods that allow a proper risk assessment are still rare. In the present study, the cytotoxicity of CeO2 nanoparticles has been assessed. For this purpose, a variety of well-defined CeO2 nanoparticles has been prepared by using either hydrothermal synthesis or flame spray pyrolysis (FSP), resulting in nanoparticles of different morphologies and sizes. The FSP technique is known to produce particles of a very small size (in the range of nanometers), which can easily become airborne. We employed a characterization procedure that makes use of physicochemical techniques, comprising N2 physisorption, XRD, TEM, as well as ζ-potential and surface-charge measurements. The cytotoxicity of the nanoparticles was evaluated in vitro on two different human lung cell lines (A549 and MRC-5). The tests showed that, despite the differences in surface properties, size, and morphologies, neither of the CeO2 samples gave rise to a cytotoxic response.

  • 143.
    Steele, A.
    et al.
    Carnegie Institution for Science, USA.
    Benning, L. G.
    German Research Centre for Geosciences, Germany; Free University of Berlin, Germany; University of Leeds, UK.
    Wirth, R.
    German Research Centre for Geosciences, Germany.
    Siljeström, Sandra
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Fries, M. D.
    NASA, USA.
    Hauri, E.
    Carnegie Institution of Washington, USA.
    Conrad, P. G.
    Carnegie Institution for Science, USA.
    Rogers, K.
    Rensselaer Polytechnic Institute, USA.
    Eigenbrode, J.
    NASA, USA.
    Schreiber, A.
    German Research Centre for Geosciences, Germany.
    Needham, A.
    USRA, USA.
    Wang, J. H.
    Carnegie Institution of Washington, USA.
    McCubbin, F. M.
    NASA, USA.
    Kilcoyne, D.
    Advanced Light Source, USA.
    Rodriguez Blanco, Juan Diego
    University of Leeds, UK.
    Organic synthesis on Mars by electrochemical reduction of CO<sub>2</sub>2018Inngår i: Science Advances, Vol. 4, nr 10, artikkel-id eaat5118Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The sources and nature of organic carbon on Mars have been a subject of intense research. Steele et al. (2012) showed that 10 martian meteorites contain macromolecular carbon phases contained within pyroxene- and olivine-hosted melt inclusions. Here, we show that martian meteorites Tissint, Nakhla, and NWA 1950 have an inventory of organic carbon species associated with fluid-mineral reactions that are remarkably consistent with those detected by the Mars Science Laboratory (MSL) mission. We advance the hypothesis that interactions among spinel-group minerals, sulfides, and a brine enable the electrochemical reduction of aqueous CO2 to organic molecules. Although documented here in martian samples, a similar process likely occurs wherever igneous rocks containing spinel-group minerals and/or sulfides encounter brines.

  • 144.
    Stenlund, Patrik
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. BIOMATCELL VINN Excellence Center of Biomaterials Cell Therapy, Sweden ; University of Gothenburg, Sweden.
    Trobos, Margarita
    BIOMATCELL VINN Excellence Center of Biomaterials Cell Therapy, Sweden ; University of Gothenburg, Sweden.
    Lausmaa, Jukka
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. BIOMATCELL VINN Excellence Center of Biomaterials Cell Therapy, Sweden .
    Brånemark, Rickard
    BIOMATCELL VINN Excellence Center of Biomaterials Cell Therapy, Sweden ; Sahlgrenska University Hospital, Sweden.
    Thomsen, Peter
    BIOMATCELL VINN Excellence Center of Biomaterials Cell Therapy, Sweden ; University of Gothenburg, Sweden.
    Palmquist, Anders
    BIOMATCELL VINN Excellence Center of Biomaterials Cell Therapy, Sweden ; University of Gothenburg, Sweden.
    Effect of load on the bone around bone-anchored amputation prostheses2017Inngår i: Journal of Orthopaedic Research, ISSN 0736-0266, E-ISSN 1554-527X, Vol. 35, nr 5, s. 1113-1122Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Osseointegrated transfemoral amputation prostheses have proven successful as an alternative method to the conventional socket-type prostheses. The method improves prosthetic use and thus increases the demands imposed on the bone-implant system. The hypothesis of the present study was that the loads applied to the bone-anchored implant system of amputees would result in locations of high stress and strain transfer to the bone tissue and thus contribute to complications such as unfavourable bone remodeling and/or elevated inflammatory response and/or compromised sealing function at the tissue-abutment interface. In the study, site-specific loading measurements were made on amputees and used as input data in finite element analyses to predict the stress and strain distribution in the bone tissue. Furthermore, a tissue sample retrieved from a patient undergoing implant revision was characterized in order to evaluate the long-term tissue response around the abutment. Within the limit of the evaluated bone properties in the present experiments, it is concluded that the loads applied to the implant system may compromise the sealing function between the bone and the abutment, contributing to resorption of the bone in direct contact with the abutment at the most distal end. This was supported by observations in the retrieved clinical sample of bone resorption and the formation of a soft tissue lining along the abutment interface. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1113–1122, 2017.

  • 145.
    Sturk, David
    et al.
    Autoliv Sverige AB, Sweden.
    Rosell, Lars
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Blomqvist, Per
    RISE - Research Institutes of Sweden, Säkerhet och transport, Safety.
    Ahlberg Tidblad, Annika
    Volvo Car Corporation, Sweden.
    Analysis of li-ion battery gases vented in an inert atmosphere thermal test chamber2019Inngår i: Batteries, ISSN 2313-0105, Vol. 5, nr 3, artikkel-id 61Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    One way to support the development of new safety practices in testing and field failure situations of electric vehicles and their lithium-ion (Li-ion) traction batteries is to conduct studies simulating plausible incident scenarios. This paper focuses on risks and hazards associated with venting of gaseous species formed by thermal decomposition reactions of the electrolyte and electrode materials during thermal runaway of the cell. A test set-up for qualitative and quantitative measurements of both major and minor gas species in the vented emissions from Li-ion batteries is described. The objective of the study is to measure gas emissions in the absence of flames, since gassing can occur without subsequent fire. Test results regarding gas emission rates, total gas emission volumes, and amounts of hydrogen fluoride (HF) and CO2 formed in inert atmosphere when heating lithium iron phosphate (LFP) and lithium nickel-manganese-cobalt (NMC) dioxide/lithium manganese oxide (LMO) spinel cell stacks are presented and discussed. Important test findings include the large difference in total gas emissions from NMC/LMO cells compared to LFP, 780 L kg−1 battery cells, and 42 L kg−1 battery cells, respectively. However, there was no significant difference in the total amount of HF formed for both cell types, suggesting that LFP releases higher concentrations of HF than NMC/LMO cells. © 2019 by the authors.

  • 146.
    Sundberg, Peter
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Grund Bäck, Lina
    RISE - Research Institutes of Sweden, Samhällsbyggnad, Byggteknik.
    Orman, Robin
    Johnson Matthey Technology Centre, UK.
    Booth, Jonathan
    Johnson Matthey Technology Centre, UK.
    Karlsson, Stefan
    RISE - Research Institutes of Sweden, Samhällsbyggnad, Byggteknik.
    Simultaneous chemical vapor deposition and thermal strengthening of glass2018Inngår i: Thin Solid Films, ISSN 0040-6090, E-ISSN 1879-2731, Vol. 669, nr 1, s. 487-493Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In the current paper we present a concept combining metal organic chemical vapor deposition with thermal strengthening process of flat glass. As the flat glass is heated to be thermally strengthened, which takes up to 20 minutes, there is an opportunity for performing a surface modification. We describe the application of transparent and amorphous Al2O3 thin films during the thermal strengthening process. Al2O3 was chosen due to the following desirable properties: increased surface mechanical properties and increased chemical durability, the latter has not been investigated in the current paper. The residual surface compressive stresses after performed strengthening of the coated glasses were quantified to be in the range of 80–110 MPa. The Al2O3 content in the surface was measured using the Surface Ablation Cell employed with Inductively Coupled Plasma Atomic Emission Spectroscopy and found to be at least doubled at the surface and having an increased Al2O3 content at least 0.5 μm underneath the glass surface. During the surface reaction, sodium is migrating to the surface giving a hazy salt layer on the glass which can easily be washed off with water. The applied coatings are transparent and provide increased surface hardness and crack resistance at low indentation loads. At higher indentation loads the interaction volume is larger and displays the same effect on the surface mechanical properties as for thermally strengthened glass. The contact angle with water compared to annealed float glass is significantly increased from 5° to 45° due to the different surface chemistry and surface topography.

    Fulltekst tilgjengelig fra 2020-11-28 08:05
  • 147.
    Sundberg, Peter
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Grund Bäck, Lina
    RISE - Research Institutes of Sweden, Samhällsbyggnad, Byggteknik.
    Orman, Robin
    Johnson Matthey Technology Centre, UK.
    Johnson, Simon R.
    Johnson Matthey Technology Centre, UK.
    Booth, Jonathan
    Johnson Matthey Technology Centre, UK.
    Karlsson, Stefan
    RISE - Research Institutes of Sweden, Samhällsbyggnad, Byggteknik.
    Novel thermo-chemical strengthening of glass for solar energy applications and its impact on the physical properties2017Konferansepaper (Fagfellevurdert)
    Abstract [en]

    The LIMES project (Light Innovative Materials for Enhanced Solar Efficiency), a Solar-ERA.NET project, have been a fruitful collaboration project to optimize many different properties in state-of-the-art solar glasses for photovoltaic (PV) modules. Here, we present results related to the effectiveness of reactive gas strengthening and its improvement of the physical properties of thin glasses. Novel thermo-chemical strengthening has been created using reactive chemicals that react with the glass surface during the thermal strengthening process. The glass surface gets a markedly increase of Al2O3, which in previous studies has been shown to have a beneficial effect on the mechanical properties. Successful thermo-chemical strengthening of 4 and 2 mm glasses to a similar strengthening level have repeatedly been performed, polariscope images in Figure 1. The strengthening level has been quantified using SCALP (Scattered Light Polariscope) and were found to be in the range of 85-110 MPa of compressive stresses in the surface which are comparable to values for conventional thermally strengthened glass.

    The strength of the glasses was quantified using the ring-on-ring method and the surface mechanical properties were evaluated by means of nano/microindentation. UV-Vis spectroscopy measurements have also been performed.

    Results for the ring-on-ring tests show that the 2 mm thin glass were positively affected by the while 4 mm did not show any significant change as compared to thermally strengthened. The thermo-chemically strengthened glasses have a significantly higher crack resistance than both the reference float glass and the traditionally thermally strengthened glass. The hardness results show that for low loads, ≥1 mN, the hardness follow the order thermo-chemically strengthened glass > thermally strengthened glass > annealed float glass. The scratch resistance for thermo-chemically strengthened glass is increased as compared to ordinary float glass. In addition, the transmission is not markedly reduced.

  • 148.
    Svanberg, Lina
    et al.
    RISE - Research Institutes of Sweden, Biovetenskap och material, Jordbruk och livsmedel.
    Malmberg, Kajsa
    RISE - Research Institutes of Sweden, Biovetenskap och material, Jordbruk och livsmedel.
    Gustinelli, Graziele
    RISE - Research Institutes of Sweden, Biovetenskap och material, Jordbruk och livsmedel. Chalmers University of Technology, Sweden.
    Öhgren, Camilla
    RISE - Research Institutes of Sweden, Biovetenskap och material, Jordbruk och livsmedel.
    Persson, Ingela
    RISE - Research Institutes of Sweden, Biovetenskap och material, Jordbruk och livsmedel.
    Brive, Lena
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Wassén, Sophia
    Effect of anthocyanins on lipid oxidation and microbial spoilage in value-added emulsions with bilberry seed oil, anthocyanins and cold set whey protein hydrogels2019Inngår i: Food Chemistry, ISSN 0308-8146, E-ISSN 1873-7072, Vol. 272, s. 273-278Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The objective of this work was to explore the storage properties of a structured oil-in-water emulsion containing both water- and fat-soluble bioactive compounds from bilberries (Vaccinium myrtillus L.). Bilberry seed oil (BSO) was dispersed in a continuous aqueous phase of anthocyanins (AC) and whey protein isolate. The microstructure was evaluated using light microscopy and the effect of anthocyanins on lipid oxidation and microbial growth was investigated. The results showed that it was possible to generate a stable emulsion structure that resisted phase separation during 25 weeks of storage. Gas chromatography–mass spectrometry measurements of the fatty acids in the BSO during storage showed that AC had a protective effect against lipid oxidation. The AC did not have an antimicrobial effect against the investigated strains Zygosaccharomyces bailii (ATCC 42476) and Aspergillus niger (ATCC 6275 (M68)).

    Fulltekst (pdf)
    fulltext
  • 149.
    Svendsen, John
    et al.
    UiT The Arctic University of Norway, Norway.
    Grant, Thomas
    University of Auckland, New Zealand.
    Rennison, David
    University of Auckland, New Zealand.
    Brimble, Margaret
    University of Auckland, New Zealand.
    Svenson, Johan
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Very Short and Stable Lactoferricin-Derived Antimicrobial Peptides: Design Principles and Potential Uses2019Inngår i: Accounts of Chemical Research, ISSN 0001-4842, E-ISSN 1520-4898, Vol. 52, nr 3, s. 749-759Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    ConspectusThe alarming rate at which micro-organisms are developing resistance to conventional antibiotics represents one of the global challenges of our time. There is currently ample space in the antibacterial drug pipeline, and scientists are trying to find innovative and novel strategies to target the microbial enemies. Nature has remained a source of inspiration for most of the antibiotics developed and used, and the immune molecules produced by the innate defense systems, as a first line of defense, have been heralded as the next source of antibiotics. Most living organisms produce an arsenal of antimicrobial peptides (AMPs) to rapidly fend off intruding pathogens, and several different attempts have been made to transform this versatile group of compounds into the next generation of antibiotics. However, faced with the many hurdles of using peptides as drugs, the success of these defense molecules as therapeutics remains to be realized. AMPs derived from the proteolytic degradation of the innate defense protein lactoferrin have been shown to display several favorable antimicrobial properties. In an attempt to investigate the biological and pharmacological properties of these much shorter AMPs, the sequence dependence was investigated, and it was shown, through a series of truncation experiments, that these AMPs in fact can be prepared as tripeptides, with improved antimicrobial activity, via the incorporation of unnatural hydrophobic residues and terminal cappings. In this Account, we describe how this class of promising cationic tripeptides has been developed to specifically address the main challenges limiting the general use of AMPs. This has been made possible through the identification of the antibacterial pharmacophore and via the incorporation of a range of unnatural hydrophobic and cationic amino acids. Incorporation of these residues at selected positions has allowed us to extensively establish how these compounds interact with the major proteolytic enzymes trypsin and chymotrypsin and also the two major drug-binding plasma proteins serum albumin and α-1 glycoprotein. Several of the challenges associated with using AMPs relate to their size, susceptibility to rapid proteolytic degradation, and poor oral bioavailability. Our studies have addressed these issues in detail, and the results have allowed us to effectively design and prepare active and metabolically stable AMPs that have been evaluated in a range of functional settings. The optimized short AMPs display inhibitory activities against a plethora of micro-organisms at low micromolar concentrations, and they have been shown to target resistant strains of both bacteria and fungi alike with a very rapid mode of action. Our Account further describes how these compounds behave in in vivo experiments and highlights both the challenges and possibilities of the intriguing compounds. In several areas, they have been shown to exhibit comparable or superior activity to established antibacterial, antifungal, and antifouling commercial products. This illustrates their ability to effectively target and eradicate various microbes in a variety of settings ranging from the ocean to the clinic.

  • 150.
    Swerin, Agne
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material. KTH Royal Institute of Technology, Sweden.
    Probing interactions of nanocellulose and sugars using rheology2017Inngår i: International Conference on Nanotechnology for Renewable Materials 2017, TAPPI Press , 2017, s. 305-314Konferansepaper (Fagfellevurdert)
1234 101 - 150 of 163
RefereraExporteraLink til resultatlisten
Permanent link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
v. 2.35.10