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  • 1.
    Afshari, Alireza
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor. Chalmers tekniska högskola, Institutionen för installationsteknik.
    Determination of VOC emissions from surface coatings by environmental test chamber measurements1999Doctoral thesis, monograph (Other academic)
  • 2.
    Alfredsson, Robert
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber.
    Jarl, Marie-Louice
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber.
    Bestämning av sammansättning hos PVC-produkter från gamla hus.1999Report (Refereed)
    Download full text (pdf)
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  • 3.
    Alriksson, Björn
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Processum.
    Eskilsson, Martin
    Holmen, Sweden.
    Johansson, Emma
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Processum.
    Lapidot, Shaul
    Melodea, Israel.
    Norström, Markus
    RISE, SP – Sveriges Tekniska Forskningsinstitut.
    Schultz-Eklund, Ola
    Holmen, Sweden .
    Shkedi, Yoram
    Melodea, Israel.
    Svedberg, Anna
    MoRe Research, Sweden.
    Svensson, Stefan
    MoRe Research, Sweden.
    Swerin, Agne
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Europe’s first pilot facility for cellulose nanocrystals (CNC)2016In: Annual Surface and Materials Chemistry Symposium and Materials for tomorrow (ASMCS 2016), 2016Conference paper (Refereed)
  • 4.
    An, Junxue
    et al.
    KTH Royal Institute of Technology, Sweden.
    Liu, Xiaoyan
    KTH Royal Institute of Technology, Sweden.
    Linse, Per
    Lund University, Sweden.
    Dedinaite, Andra
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber. KTH Royal Institute of Technology, Sweden.
    Winnik, Françoise M.
    University of Montreal, Canada; National Institute for Materials Science, Japan.
    Claesson, Per M.
    RISE, SP – Sveriges Tekniska Forskningsinstitut. KTH Royal Institute of Technology, Sweden.
    Tethered Poly(2-isopropyl-2-oxazoline) Chains: Temperature Effects on Layer Structure and Interactions Probed by AFM Experiments and Modeling2015In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 31, no 10, p. 3039-3048Article in journal (Refereed)
    Abstract [en]

    Thermoresponsive polymer layers on silica surfaces have been obtained by utilizing electrostatically driven adsorption of a cationic–nonionic diblock copolymer. The cationic block provides strong anchoring to the surface for the nonionic block of poly(2-isopropyl-2-oxazoline), referred to as PIPOZ. The PIPOZ chain interacts favorably with water at low temperatures, but above 46 °C aqueous solutions of PIPOZ phase separate as water becomes a poor solvent for the polymer. We explore how a change in solvent condition affects interactions between such adsorbed layers and report temperature effects on both normal forces and friction forces. To gain further insight, we utilize self-consistent lattice mean-field theory to follow how changes in temperature affect the polymer segment density distributions and to calculate surface force curves. We find that with worsening of the solvent condition an attraction develops between the adsorbed PIPOZ layers, and this observation is in good agreement with predictions of the mean-field theory. The modeling also demonstrates that the segment density profile and the degree of chain interpenetration under a given load between two PIPOZ-coated surfaces rise significantly with increasing temperature.

  • 5.
    Andersson, Bo-Lennart
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Klassificering av atmosfärisk korrosivitet med hjälp av referenskuponger av olika metaller. Rekommenderade rutiner.1999Report (Refereed)
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  • 6.
    Andersson, Bo-Lennart
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Carlsson, Bo
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Insamling av miljödata vid Statens Provningsanstalt i Borås.1989Report (Refereed)
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  • 7.
    Andersson, Bo-Lennart
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Carlsson, Bo
    Korrosionsskyddsklassning av organiska ytbeläggningar2000Report (Refereed)
    Download full text (pdf)
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  • 8. Andersson, J
    et al.
    Stenhamre, H
    Bäckdahl, Henrik
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Gatenholm, P
    Behaviour of human chondrocytes in engineered porous bacterial cellulose scaffolds. J Biomed Mater Res: Part A. 94A2010In: J Biomed Mater Res: Part A., Vol. 94A, no 4, p. 1124-1132Article in journal (Refereed)
  • 9. Andon, F T
    et al.
    Kapralov, A A
    Yanamala, N
    Feng, W
    Baygan, A
    Chambers, B J
    Brandner, B. D.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Biodegradation of single-walled carbon nanotubes by eosinophil peroxidase2013In: Small, ISSN 1613-6810, E-ISSN 1613-6829, Vol. 9, no 16, p. 2721-2729Article in journal (Refereed)
    Abstract [en]

    Eosinophil peroxidase (EPO) is one of the major oxidant-producing enzymes during inflammatory states in the human lung. The degradation of single-walled carbon nanotubes (SWCNTs) upon incubation with human EPO and H2O 2 is reported. Biodegradation of SWCNTs is higher in the presence of NaBr, but neither EPO alone nor H2O2 alone caused the degradation of nanotubes. Molecular modeling reveals two binding sites for SWCNTs on EPO, one located at the proximal side (same side as the catalytic site) and the other on the distal side of EPO. The oxidized groups on SWCNTs in both cases are stabilized by electrostatic interactions with positively charged residues. Biodegradation of SWCNTs can also be executed in an ex vivo culture system using primary murine eosinophils stimulated to undergo degranulation. Biodegradation is proven by a range of methods including transmission electron microscopy, UV-visible-NIR spectroscopy, Raman spectroscopy, and confocal Raman imaging. Thus, human EPO (in vitro) and ex vivo activated eosinophils mediate biodegradation of SWCNTs: an observation that is relevant to pulmonary responses to these materials. Human eosinophil peroxidase (EPO) is able to degrade SWCNTs in vitro in the presence of H2O2. EPO is one of the major oxidant-generating enzymes present in human lungs during inflammatory states. The biodegradation of SWCNTs is evidenced also in an ex vivo culture system using primary murine eosinophils stimulated to undergo degranulation. These results are relevant to potential respiratory exposure to carbon nanotubes.

  • 10.
    Arlotto, Sébastien
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber.
    Characterisation and accelerated ageing of PVC materials from old buildings.1999Report (Refereed)
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  • 11.
    Aronsson, B-O
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber.
    Lausmaa, J
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber.
    Kasemo, B
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber.
    Glow-discharge plasma treatment for surface cleaning and modificaiton of metallic biomaterials.1997In: Journal of Biomedical Materials Research, ISSN 0021-9304, E-ISSN 1097-4636, Vol. 35, no 1, p. 49-73Article in journal (Other academic)
  • 12.
    Arrhenius, Karine
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Kemi.
    Brown, Andrew S.
    NPL National Physical Laboratory, UK.
    van der Veen, Adriaan M. H.
    VSL Dutch Metrology Institute, Netherlands.
    Suitability of different containers for the sampling and storage of biogas and biomethane for the determination of the trace-level impurities - A review2016In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 902, p. 22-32Article, review/survey (Refereed)
    Abstract [en]

    The traceable and accurate measurement of biogas impurities is essential in order to robustly assess compliance with the specifications for biomethane being developed by CEN/TC408. An essential part of any procedure aiming to determinate the content of impurities is the sampling and the transfer of the sample to the laboratory. Key issues are the suitability of the sample container and minimising the losses of impurities during the sampling and analysis process. In this paper, we review the state-of-the-art in biogas sampling with the focus on trace impurities. Most of the vessel suitability studies reviewed focused on raw biogas. Many parameters need to be studied when assessing the suitability of vessels for sampling and storage, among them, permeation through the walls, leaks through the valves or physical leaks, sorption losses and adsorption effects to the vessel walls, chemical reactions and the expected initial concentration level. The majority of these studies looked at siloxanes, for which sampling bags, canisters, impingers and sorbents have been reported to be fit-for-purpose in most cases, albeit with some limitations. We conclude that the optimum method requires a combination of different vessels to cover the wide range of impurities commonly found in biogas, which have a wide range of boiling points, polarities, water solubilities, and reactivities. The effects from all the parts of the sampling line must be considered and precautions must be undertaken to minimize these effects. More practical suitability tests, preferably using traceable reference gas mixtures, are needed to understand the influence of the containers and the sampling line on sample properties and to reduce the uncertainty of the measurement.

  • 13.
    Arrhenius, Karine
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SP – Sveriges Tekniska Forskningsinstitut / Organisk kemi (Kmo).
    Kühnemuth, Daniel
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Kemi.
    Traceable reference gas mixtures for sulfur-free natural gas odorants2014In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 13, no 1, p. 6695-6702Article in journal (Refereed)
    Abstract [en]

    The first reference gas mixtures of sulfur-free natural gas odorants that are traceable to the International System of Units (SI) have been produced and their compositions validated. These mixtures, which contain methyl acrylate and ethyl acrylate at amount fractions between 1.1 and 2.1 μmol mol-1, can be used to underpin measurements of sulfur-free odorants, which are increasingly being used to odorize natural gas in transmission networks as they have less harmful properties than traditional sulfur-containing odorants. The reference gas mixtures produced have been shown to be stable in passivated aluminum cylinders for at least 8 months and have been validated (to within 6% or less) by interlaboratory measurements at three National Measurement Institutes. The stability of methyl acrylate and ethyl acrylate in gas sampling bags has been investigated, and the challenges of analyzing 2-ethyl-3- methylpyrazine, which is used as a stabilizer in sulfur-free odorants, are also briefly discussed.

  • 14.
    Arrhenius, Karine
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Podien, Doris
    RISE, SP – Sveriges Tekniska Forskningsinstitut.
    Yaghooby, Haleh
    RISE, SP – Sveriges Tekniska Forskningsinstitut.
    Smajovic, Nijaz
    RISE, SP – Sveriges Tekniska Forskningsinstitut.
    Optimization of an Analytical Method for the Measurement of Oil Carryover from a Compressor in Compressed Natural Gas Refueling Stations2015In: Energy & Fuels, ISSN 0887-0624, E-ISSN 1520-5029, Vol. 29, no 4, p. 2416-2421Article in journal (Refereed)
    Abstract [en]

    The aims of the study were to determine the best method for extracting oil absorbed on coalescing filters at compressed natural gas (CNG) refueling stations and to compare the mass spectrometer (MS) and flame ionization detector (FID) for the quantification of the oil recovered in the extracts. Dichloromethane and heptane as solvents gave slightly higher recovery yields than pentane. The preferred extraction method with regard to time and solvent consumption consisted of an ultrasonic extraction, followed by removal of the remaining solvent under a stream of nitrogen. The FID and MS were found to be equally suitable for quantifying oil carryover, if the sample only contained the target oil when the instruments of analysis have been properly calibrated. If the sample is contaminated by compounds other than the target oil, MS and FID will provide different valuable information: MS may give information on the structure of the contaminants, while FID will give a more reliable quantification without proper calibration. The work discusses issues with the reusability of the filters and how to handle the memory effects.

  • 15.
    Arvidson, Magnus
    et al.
    Brandteknik, skydd (BRs ).
    Bäckman, Anna
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Hedlund, Hans-Peter
    Källqvist, Sofia
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Påverkan av vatten från sprinkler på vägg- och takmålningar2007Report (Refereed)
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  • 16.
    Arvidson, Magnus
    et al.
    Brandteknik, skydd (BRs ).
    Månsson, Margret
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Utvärdering av olika frysskyddsmedel för sprinklersystem. Brandforsk projekt 631-9611999Report (Refereed)
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  • 17.
    Badal Tejedor, Maria
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor. KTH Royal Institute of Technology, Sweden.
    Nordgren, Niklas
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Schuleit, Michael
    Novartis Pharma AG, Switzerland.
    Rutland, Mark W.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor. KTH Royal Institute of Technology, Sweden.
    Millqvist-Fureby, Anna
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Tablet mechanics depend on nano and micro scale adhesion, lubrication and structure2015In: International Journal of Pharmaceutics, ISSN 0378-5173, E-ISSN 1873-3476, Vol. 486, no 1-2, p. 315-323Article in journal (Refereed)
    Abstract [en]

    Tablets are the most convenient form for drug administration. However, despite the ease of manufacturing problems such as powder adhesion occur during the production process. This study presents surface and structural characterization of tablets formulated with commonly used excipients (microcrystalline cellulose (MCC), lactose, mannitol, magnesium (Mg) stearate) pressed under different compaction conditions. Tablet surface analyses were performed with scanning electron microscopy (SEM), profilometry and atomic force microscopy (AFM). The mechanical properties of the tablets were evaluated with a tablet hardness test. Local adhesion detected by AFM decreased when Mg stearate was present in the formulation. Moreover, the tablet strength of plastically deformable excipients such as MCC was significantly decreased after addition of Mg stearate. Combined these facts indicate that Mg stearate affects the particle-particle bonding and thus elastic recovery. The MCC excipient also displayed the highest hardness which is characteristic for a highly cohesive material. This is discussed in the view of the relatively high adhesion found between MCC and a hydrophilic probe at the nanoscale using AFM. In contrast, the tablet strength of brittle materials like lactose and mannitol is unaffected by Mg stearate. Thus fracture occurs within the excipient particles and not at particle boundaries, creating new surfaces not previously exposed to Mg stearate. Such uncoated surfaces may well promote adhesive interactions with tools during manufacture.

  • 18.
    Berggren, Carina
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber.
    Markinger, Monica
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber.
    Spricktillväxt i styv PVC.1989Report (Refereed)
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  • 19.
    Berglin, Mattias
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Gold-nanoparticle-assisted self-assembly of chemical gradients with tunable sub-50 nm molecular domains2014In: Particle & particle systems characterization, ISSN 0934-0866, E-ISSN 1521-4117, Vol. 31, no 2, p. 209-218Article in journal (Refereed)
    Abstract [en]

    A simple and efficient principle for nanopatterning with wide applicability in the sub-50 nanometer regime is chemisorption of nanoparticles; at homogeneous substrates, particles carrying surface charge may spontaneously self-organize due to the electrostatic repulsion between adjacent particles. Guided by this principle, a method is presented to design, self-assemble, and chemically functionalize gradient nanopatterns where the size of molecular domains can be tuned to match the level corresponding to single protein binding events. To modulate the binding of negatively charged gold nanoparticles both locally (<100 nm) and globally (>100 μm) onto a single modified gold substrate, ion diffusion is used to achieve spatial control of the particles' mutual electrostatic interactions. By subsequent tailoring of different molecules to surface-immobilized particles and the void areas surrounding them, nanopatterns are obtained with variable chemical domains along the gradient surface. Fimbriated Escherichia coli bacteria are bound to gradient nanopatterns with similar molecular composition and macroscopic contact angle, but different sizes of nanoscopic presentation of adhesive (hydrophobic) and repellent poly(ethylene) glycol (PEG) domains. It is shown that small hydrophobic domains, similar in size to the diameter of the bacterial fimbriae, supported firmly attached bacteria resembling catch-bond binding, whereas a high number of loosely adhered bacteria are observed on larger hydrophobic domains. Chemical gradients with the resolution needed to address complex biological binding events at the single protein level are prepared using surface-deposited gold nanoparticles as a versatile template for orthogonal chemicalmodifications. The effect of hydrophobic domain arrangement on the sub-50 nm scale is shown to influence binding of fimbriae carrying E. coli bacteria. 

  • 20.
    Berglin, Mattias
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Gradients in surface nanotopography used to study platelet adhesion and activation2013In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 110, no 1, p. 261-269Article in journal (Refereed)
  • 21.
    Berglin, Mattias
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Pellet formation of zygomycetes and immobilization of yeast2013In: New Biotechnology, ISSN 1871-6784, E-ISSN 1876-4347, Vol. 30, no 5, p. 516-522Article in journal (Refereed)
  • 22.
    Besharat, Zahra
    et al.
    KTH Royal Institute of Technology, Sweden.
    Wakeham, Deborah
    KTH Royal Institute of Technology, Sweden.
    Johnson, C. Magnus
    KTH Royal Institute of Technology, Sweden.
    Luengo, Gustavo S.
    L’Oréal Research and Innovation, France.
    Greaves, Andrew
    L’Oréal Research and Innovation, France.
    Odnevall Wallinder, Inger
    KTH Royal Institute of Technology, Sweden.
    Göthelid, Mats
    KTH Royal Institute of Technology, Sweden.
    Rutland, Mark W.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Life Science. KTH Royal Institute of Technology, Sweden.
    Mixed monolayers of alkane thiols with polar terminal group on gold: Investigation of structure dependent surface properties2016In: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 484, p. 279-290Article in journal (Refereed)
    Abstract [en]

    Adsorption of thiols with cationic or anionic terminal group on gold has been studied from mixed solutions of 11-Amino-1-undecanethiol (AUT) and 3-Mercaptopropionic acid (MPA) using Quartz Crystal Microbalance with Dissipation (QCM-D), X-ray Photoelectron Spectroscopy (XPS), atomic force microscopy (AFM) and contact angles. The goal is to probe the nature of such layers, and the additivity or otherwise of the pH responsiveness, with a view to evaluate their suitability as smart materials. For each of the two pure (unmixed) cases, ordered molecular monolayers are formed with sulfur binding to gold and the alkane chain pointing out from the surface as expected. Adsorption from the thiol mixtures, however, leads to a more complex behaviour. The surface concentration of thiols from the mixtures, as determined by QCM-D, is considerably lower than for the pure cases and it reaches a minimum at a 3:1 MPA/AUT relative concentration in the solution. The XPS results confirm a reduction in adsorbed amount in mixtures with the lowest overall intensity for the 3:1 ratio. Monolayers formed from mixtures display a wettability which is much lower and less pH sensitive. Collectively these results confirm that for adsorption from mixed systems, the configuration is completely different. Complex formation in the mixed solutions leads to the adsorption of molecules parallel to the surface in an axially in-plane configuration. This parallel layer of thiols is mechanically relatively robust to nano-shaving based on AFM measurements. These results will have a significant impact on the design of biomimetic surface coatings particularly when mixtures of oppositely charged molecules are present on the surface, as is commonly the case in biological, proteinaceous surfaces (e.g. hair and skin).

  • 23.
    Bhattacharya, Kunal
    et al.
    Karolinska Institute, Sweden.
    El-Sayed, Ramy
    Karolinska Institute, Sweden.
    Andón, Fernando T.
    Karolinska Institute, Sweden.
    Mukherjee, Sourav P
    Karolinska Institute, Sweden.
    Gregory, Joshua
    Karolinska Institute, Sweden.
    Li, Hu
    Uppsala University, Sweden.
    Zhao, Yichen
    KTH Royal Institute of Technology, Sweden.
    Seo, Wanji
    University of Pittsburgh, US.
    Fornara, Andrea
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Brandner, Birgit
    RISE, SP – Sveriges Tekniska Forskningsinstitut.
    Toprak, Muhammet S.
    KTH Royal Institute of Technology, Sweden.
    Leifer, Klaus
    Uppsala University, Sweden.
    Star, Alexander
    University of Pittsburgh, USA.
    Fadeel, Bengt
    Karolinska Institute, Sweden.
    Lactoperoxidase-mediated degradation of single-walled carbon nanotubes in the presence of pulmonary surfactant2015In: Carbon, ISSN 0008-6223, E-ISSN 1873-3891, Vol. 91, p. 506-517Article in journal (Refereed)
    Abstract [en]

    Carbon nanotubes (CNTs) may elicit inflammatory responses following pulmonary exposure. Conversely, enzymatic biodegradation of CNTs by inflammatory cells has also been reported. The aim of this study was to study the degradation of oxidized single-walled CNTs (ox-SWCNTs) by lactoperoxidase (LPO), a secreted peroxidase present in the airways, and whether pulmonary surfactant affects this biodegradation. To this end, ox-SWCNTs were incubated in vitro with recombinant bovine LPO + H2O2 + NaSCN in the presence and absence of porcine lung surfactant (Curosurf®) and biodegradation was monitored using UV-Vis-NIR spectroscopy, Raman spectroscopy, and scanning electron microscopy. The interaction of recombinant LPO with bundles of ox-SWCNTs was confirmed by atomic force microscopy. Cell-free biodegradation of ox-SWCNTs was also observed ex vivo in murine bronchoalveolar lavage fluid in the presence of H2O2 + NaSCN. Our study provides evidence for biodegradation of ox-SWCNTs with a lung surfactant 'bio-corona' and expands the repertoire of mammalian peroxidases capable of biodegradation of ox-SWCNTs. These findings are relevant to inhalation exposure to these materials, as LPO serves as an important component of the airway defense system.

  • 24.
    Björn, Camilla
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. University of Gothenburg, Sweden; Sahlgrenska Academy, Sweden.
    Antimicrobial peptides in the treatment of infectious and inflammatory conditions: Preclinical studies of mechanism of action, efficacy, and safety2016Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    The rapid emergence of antibiotic-resistant microbes worldwide and the urgent need of new antimicrobial agents have stimulated interest in antimicrobial peptides (AMPs) as new therapeutics for treatment of infectious diseases. AMPs are present in all living species and constitute an important part of the innate immune system in multicellular organisms, including humans. AMPs display a remarkably broad spectrum of antimicrobial activity covering both Gram-positive and Gram-negative bacteria, including many antibiotic-resistant strains, as well as fungi, viruses, and protozoa. Further, in contrast to many conventional antibiotics, AMPs rapidly kill bacteria instead of just inhibiting bacterial growth. In addition, AMPs act as modulators of the innate immune system and, importantly, bacteria seem less efficient in developing resistance towards AMPs than towards conventional antibiotics. Together these properties make AMPs highly attractive as a new class of antimicrobials, with clinical potential also extending to diseases where inflammation is part of the pathology. The aim of this thesis was to study novel AMPs with respect to their mechanism of action (MOA), antimicrobial spectrum, propensity to select for resistance, and in vivo efficacy and safety. To achieve this, we used a number of in vitro and in vivo assays, together generating a comprehensive preclinical evaluation of the peptides. The hypothesis was that the AMPs in this thesis have potential to be developed as therapeutic agents for several infectious and inflammatory conditions, including treatment of skin and soft tissue infections and prevention of postsurgical adhesion formation. The results showed that all AMPs tested (i.e. PXL03, PXL150, HLR1r, and five variants of CEN1 HC-Br) had broad antimicrobial spectra in vitro with varying sensitivity to salt and serum. Furthermore, PXL150 caused a rapid permeabilization of bacterial membrane in vitro, indicating that this is at least one part of the MOA of this peptide. Under selection pressure in vitro, bacteria did not develop resistance to the peptides tested, i.e. PXL150 and CEN1 HC. Interestingly, all peptides showed anti-inflammatory activity by inhibiting the secretion of proinflammatory mediators from stimulated human cell lines. In addition, PXL01, PXL150, and HLR1r demonstrated fibrinolytic ability in vitro by suppressing the release of plasminogen activator inhibitor-1 (PAI-1). In ex vivo and in vivo skin/wound infection models, the peptides reduced the number of viable bacteria and yeast cells. Further, PXL01 decreased postsurgical adhesion formation in vivo. Notably, nonclinical safety studies showed that PXL150 was safe and well tolerated. In conclusion, several of the peptides evaluated in this thesis demonstrated a promising preclinical efficacy and safety profile motivating further development as drug candidates for local treatment of infectious and inflammatory conditions.

  • 25.
    Björn, Camilla
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. Pergamum AB, Sweden; University of Gothenburg, Sweden.
    Mahlapuu, Margit
    Pergamum AB, Sweden; University of Gothenburg, Sweden.
    Mattsby-Baltzer, Inger
    University of Gothenburg, Sweden.
    Håkansson, Joakim
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. Pergamum AB, Sweden.
    Anti-infective efficacy of the lactoferrin-derived antimicrobial peptide HLR1r2016In: Peptides, ISSN 0196-9781, E-ISSN 1873-5169, Vol. 81, p. 21-28Article in journal (Refereed)
    Abstract [en]

    Antimicrobial peptides (AMPs) have emerged as a new class of drug candidates for the treatment of infectious diseases. Here we describe a novel AMP, HLR1r, which is structurally derived from the human milk protein lactoferrin and demonstrates a broad spectrum microbicidal action in vitro. The minimum concentration of HLR1r needed for killing ≥99% of microorganisms in vitro, was in the range of 3-50 μg/ml for common Gram-negative and Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), and for the yeast Candida albicans, when assessed in diluted brain-heart infusion medium. We found that HLR1r also possesses anti-inflammatory properties as evidenced by inhibition of tumor necrosis factor alpha (TNF-α) secretion from human monocyte-derived macrophages and by repression of interleukin-6 (IL-6) and plasminogen activator inhibitor-1 (PAI-1) secretion from human mesothelial cells, without any cytotoxic effect observed at the concentration range tested (up to 400 μg/ml). HLR1r demonstrated pronounced anti-infectious effect in in vivo experimental models of cutaneous candidiasis in mice and of excision wounds infected with MRSA in rats as well as in an ex vivo model of pig skin infected with S. aureus. In conclusion, HLR1r may constitute a new therapeutic alternative for local treatment of skin infections.

  • 26.
    Björn, Camilla
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. Pergamum AB, Sweden; University of Gothenburg, Sweden.
    Noppa, Lalia
    FOI Swedish Defence Research Agency, Sweden.
    Näslund Salomonsson, Emelie
    FOI Swedish Defence Research Agency, Sweden.
    Johansson, Anna-Lena
    FOI Swedish Defence Research Agency, Sweden.
    Nilsson, Elin
    FOI Swedish Defence Research Agency, Sweden.
    Mahlapuu, Margit
    Pergamum AB, Sweden; University of Gothenburg, Sweden.
    Håkansson, Joakim
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. Pergamum AB, Sweden.
    Efficacy and safety profile of the novel antimicrobial peptide PXL150 in a mouse model of infected burn wounds2015In: International Journal of Antimicrobial Agents, ISSN 0924-8579, E-ISSN 1872-7913, Vol. 45, no 5, p. 519-524Article in journal (Refereed)
    Abstract [en]

    The urgent need to develop novel antimicrobial therapies has stimulated interest in antimicrobial peptides as therapeutic candidates for the treatment of infectious diseases. The aim of this study was to evaluate the anti-infectious effect of the synthetic antimicrobial peptide PXL150, formulated in hydroxypropyl cellulose (HPC) gel, on Pseudomonas aeruginosa in vitro and in an in vivo mouse model of infected burn wounds as well as to assess the in vivo safety profile of PXL150 in rats and rabbits. Minimal microbicidal concentration analysis showed prominent efficacy of PXL150 against P. aeruginosa in vitro, which was further enhanced in formulating the peptide in HPC gel. Application of 1.25, 2.5, 5, 10 and 20 mg/g PXL150 in HPC gel twice daily for four consecutive days significantly reduced bacterial counts in the burn wounds compared with non-treated or placebo-treated controls. Continuous bioluminescence measurements of the bacteria revealed a pronounced anti-infective effect already at the first day post infection by PXL150 in concentrations of ≥2.5 mg/g. In the non-clinical safety studies, PXL150 showed a favourable safety profile following repeated administration systemically and locally in rats and rabbits, respectively. In conclusion, these data support that PXL150 has the potential to be an effective and safe drug candidate for the treatment of infected burn wounds. The findings encourage the progression of PXL150 as a novel topical treatment of microbial infections.

  • 27.
    Blomberg, Eva
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Adsorption of lysozyme on silver and its influence on silver release2014In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 30, no 46, p. 13877-13889Article in journal (Refereed)
  • 28.
    Blomberg, Eva
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Chemical, mechanical and antibacterial properties of silver nanocluster/silica composite coated textiles for safety systems and aerospace applications2014In: Applied Surface Science, ISSN 0169-4332, E-ISSN 1873-5584, Vol. 317, no Oct, p. 131-139Article in journal (Refereed)
  • 29.
    Blomberg, Eva
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Correlation between surface physicochemical properties and the release of iron from stainless steel AISI 304 in biological media2014In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 122, no Oct, p. 216-222Article in journal (Refereed)
  • 30.
    Blomberg, Eva
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Effect of laundry surfactants on surface charge and colloidal stability of silver nanoparticles2013In: Langmuir, Vol. 29, no 28, p. 8882-8891Article in journal (Refereed)
  • 31.
    Blomqvist, P
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Månsson, M
    Fire characteristics and detailed smoke gas analyses in controlled underventilated large-scale combustion experiments.1996In: Industrial fires III, Workshop Proceedings, European commission, Risø, Denmark, 17-18 september 1996., European Commission , 1996, , p. 7-16Conference paper (Other academic)
  • 32.
    Blomqvist, Per
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SP Sveriges tekniska forskningsinstitut / Brandteknik, forskning (BRf ).
    Lindberg, Per
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Certifiering, Ledningssystemcertifiering.
    Månsson, Margret
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    TOXFIRE - Fire characteristics and smoke gas analyses in under-ventilated large-scale combustion experments. FTIR measurements.1996Report (Refereed)
    Download full text (pdf)
    FULLTEXT01
  • 33.
    Bobert, Magnus
    et al.
    Brandteknik, skydd (BRs ).
    Buren, Anneli
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber.
    Spilg, Leo
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber.
    Andersson, Petra
    Brandteknik, forskning (BRf ).
    Framtagning av underlag till komponentprovning av linjevärmedetektorer för fasadmontering2007Report (Refereed)
    Abstract [sv]

    Suggestions for how to test and verify line type heat detectors suitable for facade mounting are given. Related standards including prEN54:22 are discussed. Key words: Linjevärmedetektorer, komponentprovningar, beständighetstest, funktionstest

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    FULLTEXT01
  • 34.
    Boge, Lukas
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Life Science. Chalmers University of Technology, Sweden.
    Bysell, Helena
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Life Science.
    Ringstad, Lovisa
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Life Science.
    Wennman, David
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Process Development, Analys och fastfas.
    Umerska, Anita
    University of Angers, France.
    Cassisa, Viviane
    CHU Angers, France.
    Eriksson, Jonny
    Uppsala University, Sweden.
    Joly-Guillou, Marie-Laure
    CHU Angers, France.
    Edwards, Katarina
    Uppsala University, Sweden.
    Andersson, Martin
    Chalmers University of Technology, Sweden.
    Lipid-based liquid crystals as carriers for antimicrobial peptides: Phase behavior and antimicrobial effect2016In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 32, no 17, p. 4217-4228Article in journal (Refereed)
    Abstract [en]

    The number of antibiotic-resistant bacteria is increasing worldwide, and the demand for novel antimicrobials is constantly growing. Antimicrobial peptides (AMPs) could be an important part of future treatment strategies of various bacterial infection diseases. However, AMPs have relatively low stability, because of proteolytic and chemical degradation. As a consequence, carrier systems protecting the AMPs are greatly needed, to achieve efficient treatments. In addition, the carrier system also must administrate the peptide in a controlled manner to match the therapeutic dose window. In this work, lyotropic liquid crystalline (LC) structures consisting of cubic glycerol monooleate/water and hexagonal glycerol monooleate/oleic acid/water have been examined as carriers for AMPs. These LC structures have the capability of solubilizing both hydrophilic and hydrophobic substances, as well as being biocompatible and biodegradable. Both bulk gels and discrete dispersed structures (i.e., cubosomes and hexosomes) have been studied. Three AMPs have been investigated with respect to phase stability of the LC structures and antimicrobial effect: AP114, DPK-060, and LL-37. Characterization of the LC structures was performed using small-angle X-ray scattering (SAXS), dynamic light scattering, ζ-potential, and cryogenic transmission electron microscopy (Cryo-TEM) and peptide loading efficacy by ultra performance liquid chromatography. The antimicrobial effect of the LCNPs was investigated in vitro using minimum inhibitory concentration (MIC) and time-kill assay. The most hydrophobic peptide (AP114) was shown to induce an increase in negative curvature of the cubic LC system. The most polar peptide (DPK-060) induced a decrease in negative curvature while LL-37 did not change the LC phase at all. The hexagonal LC phase was not affected by any of the AMPs. Moreover, cubosomes loaded with peptides AP114 and DPK-060 showed preserved antimicrobial activity, whereas particles loaded with peptide LL-37 displayed a loss in its broad-spectrum bactericidal properties. AMP-loaded hexosomes showed a reduction in antimicrobial activity.

  • 35.
    Bogren, Maria
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber.
    Linde, Maria
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber.
    Inverkan av upprepad bearbetning med mellanliggande accelererad åldring på en ostabiliserad LDPE.1993Report (Refereed)
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    fulltext
  • 36.
    Boldizar, Antal
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber.
    Markinger, Monica
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber.
    Henriksson, Lars-Åke
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Polymer och fiber.
    Metoder för verifiering av utrustningskriterier för innebandy. *SP-Metod 1506*.1993Report (Refereed)
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    fulltext
  • 37. Boldizar, Antal
    et al.
    Möller, Kenneth
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Degradation of ABS during repeated processing and accelerated ageing2003In: Polymer Degradation Stab., Vol. 81, p. 359-366Article in journal (Refereed)
    Abstract [en]

    Degradation of ABS during repeated processing and accelerated ageing

  • 38.
    Booth, Andy
    et al.
    SINTEF, Norway.
    Størseth, Trond
    SINTEF, Norway.
    Altin, Dag
    BioTrix, Norway.
    Fornara, Andrea
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Ahniyaz, Anwar
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Jungnickel, Harald
    BfR German Federal Institute for Risk Assessment, Germany.
    Laux, Peter
    BfR German Federal Institute for Risk Assessment, Germany.
    Luch, Andreas
    BfR German Federal Institute for Risk Assessment, Germany.
    Sørensen, Lisbet
    SINTEF, Norway.
    Freshwater dispersion stability of PAA-stabilised cerium oxide nanoparticles and toxicity towards Pseudokirchneriella subcapitata2015In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 505, p. 596-605Article in journal (Refereed)
    Abstract [en]

    An aqueous dispersion of poly (acrylic acid)-stabilised cerium oxide (CeO2) nanoparticles (PAA-CeO2) was evaluated for its stability in a range of freshwater ecotoxicity media (MHRW, TG 201 and M7), with and without natural organic matter (NOM). In a 15day dispersion stability study, PAA-CeO2 did not undergo significant aggregation in any media type. Zeta potential varied between media types and was influenced by PAA-CeO2 concentration, but remained constant over 15days. NOM had no influence on PAA-CeO2 aggregation or zeta potential. The ecotoxicity of the PAA-CeO2 dispersion was investigated in 72h algal growth inhibition tests using the freshwater microalgae Pseudokirchneriella subcapitata. PAA-CeO2 EC50 values for growth inhibition (GI; 0.024mg/L) were 2-3 orders of magnitude lower than pristine CeO2 EC50 values reported in the literature. The concentration of dissolved cerium (Ce3+/Ce4+) in PAA-CeO2 exposure suspensions was very low, ranging between 0.5 and 5.6μg/L. Free PAA concentration in the exposure solutions (0.0096-0.0384mg/L) was significantly lower than the EC10 growth inhibition (47.7mg/L) value of pure PAA, indicating that free PAA did not contribute to the observed toxicity. Elemental analysis indicated that up to 38% of the total Cerium becomes directly associated with the algal cells during the 72h exposure. TOF-SIMS analysis of algal cell wall compounds indicated three different modes of action, including a significant oxidative stress response to PAA-CeO2 exposure. In contrast to pristine CeO2 nanoparticles, which rapidly aggregate in standard ecotoxicity media, PAA-stabilised CeO2 nanoparticles remain dispersed and available to water column species. Interaction of PAA with cell wall components, which could be responsible for the observed biomarker alterations, could not be excluded. This study indicates that the increased dispersion stability of PAA-CeO2 leads to an increase in toxicity compared to pristine non-stabilised forms.

  • 39.
    Bornehag, Carl-Gustaf
    et al.
    Byggnadsfysik och innemiljö (ETi ).
    Sundell, Jan
    Sigsgaard, Torben
    Hägerhed Engman, Linda
    Byggnadsfysik och innemiljö (ETi ).
    Lundgren, Björn
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Phthalates in indoor dust and their association with building characteristics, DBH-phase II2005In: Proceeding of 10th international conference on indoor air quality and climate, Bejing, China, 2005Conference paper (Refereed)
  • 40.
    Brandner, Birgit
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Effects of water gradients and use of urea on skin ultrastructure evaluated by confocal Raman microspectroscopy2013In: Biochimica et Biophysica Acta - Biomembranes, ISSN 0005-2736, E-ISSN 1879-2642, Vol. 1828, no 11, p. 2470-2478Article in journal (Refereed)
  • 41.
    Brandner, Birgit
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Claesson, Per
    RISE, SP – Sveriges Tekniska Forskningsinstitut.
    Swerin, Agne
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Hydrophobic pore array surfaces: Wetting and interaction forces in water/ethanol mixtures2013In: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 396, p. 278-286Article in journal (Refereed)
  • 42.
    Brink, M
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Jonson, H
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Sundahl, M
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Catalysis of triplet state cis-trans isomerizations making a quantum chain process more efficient1998In: Journal of Photochemistry and Photobiology A: Chemistry, ISSN 1010-6030, E-ISSN 1873-2666, Vol. 112, no 2, p. 149-153Article in journal (Other academic)
  • 43.
    Brive, Lena
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SP – Sveriges Tekniska Forskningsinstitut / Organisk kemi (Kmo).
    Pinori, Emiliano
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Multi-seasonal barnacle (Balanus improvisus) protection achieved by trace amounts of a macrocyclic lactone (ivermectin) included in rosin-based coatings2011In: Biofouling (Print), ISSN 0892-7014, E-ISSN 1029-2454, Vol. 27, no 9, p. 941-953Article in journal (Refereed)
  • 44.
    Buker, O.
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Mätteknik, Volym, flöde, temperatur o densitet.
    Rasmussen, K.
    FORCE Technology, Denmark.
    Kondrup, J. B.
    FORCE Technology, Denmark.
    Lucas, P.
    VSL Dutch Metrology Institute, The Netherlands.
    Pelevic, N.
    VSL Dutch Metrology Institute, The Netherlands.
    Stolt, K.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Mätteknik, Volym, flöde, temperatur o densitet.
    van der Beek, M.
    VSL Dutch Metrology Institute, The Netherlands.
    Arrhenius, K.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Kemi.
    Li, J.
    VSL Dutch Metrology Institute, The Netherlands.
    Lucassen, A.
    PTB Physikalisch-Technische Bundesanstalt, Germany.
    Klare, L.
    Technische Universität Braunschweig, Germany.
    Eilts, P.
    Technische Universität Braunschweig, Germany.
    Giuliano Albo, P. A.
    INRIM Istituto Nazionale di Ricerca Metrologica, Italy.
    Richter, M.
    RUB Ruhr-Universität Bochum, Germany.
    Gieseking, B.
    NPL National Physical Laboratory, UK.
    Nieuwenkamp, G.
    PTB Physikalisch-Technische Bundesanstalt, Germany.
    Rauch, J.
    VSL Dutch Metrology Institute, The Netherlands.
    Mortensen, T.
    Justervesenet, Norway.
    Metrological support for LNG custody transfer and transport2016In: Proceedings of the 17th International Flow Measurement Conference (FLOMEKO 2016), 2016, article id E943220FConference paper (Refereed)
    Abstract [en]

    In the framework of the ongoing EMRP Joint Research Project (JRP) ENG 60 “Metrology for LNG” (2014-2017), co-funded by the European Union, a number of metrological challenges associated with custody transfer and transport of LNG will be faced. The project consists of four technical work packages (WP), whereby the main objective is to reduce the measurement uncertainty of LNG custody transfer by a factor two. The focus in WP1 is the design and development of a traceable mid-scale calibration standard for LNG mass and volume flow. The goal is to provide traceable mass and volume flow calibrations up to 400 m3/h (180000 kg/h). In WP2, the emphasis is on the development and validation of a LNG sampling and composition measurement reference standard, consisting of sampler, vaporizer, gas standards, and gas chromatography (GC), which will be used to  test and calibrate commercially available LNG sampling and composition measurement systems. The priority in WP3 is given to the development and validation of a method for the determination of the methane number, including correlations based on the LNG composition and corrections for traces of nitrogen and higher hydrocarbons. Since physical properties and quantities play an important role in LNG custody transfer, WP4 comprises reference quality density measurements of LNG to validate and improve models for LNG density predictions, the uncertainty evaluation of enthalpy and calorific value calculations and the development of a novel cryogenic sensor for the simultaneous measurement of speed-of-sound and density. The present paper gives an overview of recently achieved objectives within the project and provides an outlook to future activities.

  • 45.
    Bäckdahl, Henrik
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. Chalmers University of Technology, Sweden.
    Risberg, B
    Sahlgrenska University Hospital, Sweden.
    Gatenholm, P
    Chalmers University of Technology, Sweden.
    Observations on bacterial cellulose tube formation for application as vascular graft2011In: Materials Science and Engineering, Vol. 31, p. 14-21Article in journal (Refereed)
    Abstract [en]

    Nanocellulose (bacterial cellulose, BC), such as that produced by Acetobacter xylinum, has shown promising results as a replacement material for small diameter vascular grafts. The surface morphology of the lumen and mechanical properties of such tubes are crucial for their performance. The growth of a BC tube in a vertical fermentation bioreactor using silicone tubing for support and as an oxygen delivery membrane has not been studied in detail previously. Oxygen concentration and the number of bacteria added influence the production of the BC tubes. A dense and smooth luminal surface was formed after 4 days on a 3 mm silicone support. The bacteria were found to be in high concentration close to the silicon support and decreased in number further away. In the region with a high bacteria concentration, dense thin layers of BC were formed since the bacteria moved close together in this region. The presented observations were summarized in a theoretical model of BC tube growth.

  • 46.
    Bäckman, Anna
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Dirt-Repellent Automotive Coatings (DRACO),2005Report (Refereed)
  • 47.
    Carlred, Louise
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. Chalmers University of Technology, Sweden.
    Michno, Wojciech
    University of Gothenburg, Sweden.
    Kaya, Ibrahim
    University of Gothenburg, Sweden.
    Sjövall, Peter
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. Chalmers University of Technology, Sweden.
    Syvänen, Stina
    Uppsala University, Sweden.
    Hanrieder, Jörg
    University of Gothenburg, Sweden; Chalmers University of Technology, Sweden; University College London, UK.
    Probing amyloid-β pathology in transgenic Alzheimer's disease (tgArcSwe) mice using MALDI imaging mass spectrometry2016In: Journal of Neurochemistry, ISSN 0022-3042, E-ISSN 1471-4159, Vol. 138, no 3, p. 469-478Article in journal (Refereed)
    Abstract [en]

    The pathological mechanisms underlying Alzheimer's disease (AD) are still not understood. The disease pathology is characterized by the accumulation and aggregation of amyloid-β (Aβ) peptides into extracellular plaques, however the factors that promote neurotoxic Aβ aggregation remain elusive. Imaging mass spectrometry (IMS) is a powerful technique to comprehensively elucidate the spatial distribution patterns of lipids, peptides and proteins in biological tissues. In the present study, matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS)-based imaging was used to study Aβ deposition in transgenic mouse brain tissue and to elucidate the plaque-associated chemical microenvironment. The imaging experiments were performed in brain sections of transgenic Alzheimer's disease mice carrying the Arctic and Swedish mutation of amyloid-beta precursor protein (tgArcSwe). Multivariate image analysis was used to interrogate the IMS data for identifying pathologically relevant, anatomical features based on their chemical identity. This include cortical and hippocampal Aβ deposits, whose amyloid peptide content was further verified using immunohistochemistry and laser microdissection followed by MALDI MS analysis. Subsequent statistical analysis on spectral data of regions of interest revealed brain region-specific differences in Aβ peptide aggregation. Moreover, other plaque-associated protein species were identified including macrophage migration inhibitory factor suggesting neuroinflammatory processes and glial cell reactivity to be involved in AD pathology. The presented data further highlight the potential of IMS as a powerful approach in neuropathology. Hanrieder et al. described an imaging mass spectrometry based study on comprehensive spatial profiling of C-terminally truncated Aβ species within individual plaques in tgArcSwe mice. Here, brain region-dependent differences in Aβ truncation and other plaque-associated proteins, such as macrophage migration inhibitory factor, were observed. The data shed further light on plaque-associated molecular mechanisms implicated in Alzheimer's pathogenesis.

  • 48.
    Carlred, Louise
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Sjövall, Peter
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SP – Sveriges Tekniska Forskningsinstitut / Funktionella material (KMf).
    Simultaneous imaging of amyloid-β and lipids in brain tissue using antibody-coupled liposomes and time-of-flight secondary ion mass spectrometry2014In: Journal of the American Chemical Society, ISSN 0002-7863, E-ISSN 1520-5126, Vol. 136, no 28, p. 9973-9981Article in journal (Refereed)
    Abstract [en]

    The spatial localization of amyloid-β peptide deposits, the major component of senile plaques in Alzheimer's disease (AD), was mapped in transgenic AD mouse brains using time-of-flight secondary ion mass spectrometry (ToF-SIMS), simultaneously with several endogenous molecules that cannot be mapped using conventional immunohistochemistry imaging, including phospholipids, cholesterol and sulfatides. Whereas the endogenous lipids were detected directly, the amyloid-β deposits, which cannot be detected as intact entities with ToF-SIMS because of extensive ion-induced fragmentation, were identified by specific binding of deuterated liposomes to antibodies directed against amyloid-β. Comparative investigation of the amyloid-β deposits using conventional immunohistochemistry and fluorescence microscopy suggests similar sensitivity but a more surface-confined identification due to the shallow penetration depth of the ToF-SIMS signal. The recorded ToF-SIMS images thus display the localization of lipids and amyloid-β in a narrow (∼10 nm) two-dimensional plane at the tissue surface. As compared to a frozen nontreated tissue sample, the liposome preparation protocol generally increased the signal intensity of endogenous lipids, likely caused by matrix effects associated with the removal of salts, but no severe effects on the tissue integrity and the spatial distribution of lipids were observed with ToF-SIMS or scanning electron microscopy (SEM). This method may provide an important extension to conventional tissue imaging techniques to investigate the complex interplay of different kinds of molecules in neurodegenerative diseases, in the same specimen. However, limitations in target accessibility of the liposomes as well as unspecific binding need further consideration.

  • 49.
    Carlred, Louise
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. Chalmers University of Technology, Sweden.
    Vukojević, Vladana
    Karolinska Institute, Sweden.
    Johansson, Björn
    Karolinska Institute, Sweden.
    Schalling, Martin
    Karolinska Institute, Sweden.
    Höök, Fredrik
    Chalmers University of Technology, Sweden.
    Sjövall, Peter
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. Chalmers University of Technology, Sweden.
    Imaging of amyloid-β in alzheimer’s disease transgenic mouse brains with ToF-SIMS using immunoliposomes2016In: Biointerphases, ISSN 1934-8630, E-ISSN 1559-4106, Vol. 11, no 2, p. 1-11, article id 02A312Article in journal (Refereed)
    Abstract [en]

    Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has been proven to successfully image different kinds of molecules, especially a variety of lipids, in biological samples. Proteins, however, are difficult to detect as specific entities with this method due to extensive fragmentation. To circumvent this issue, the authors present in this work a method developed for detection of proteins using antibody-conjugated liposomes, so called immunoliposomes, which are able to bind to the specific protein of interest. In combination with the capability of ToF-SIMS to detect native lipids in tissue samples, this method opens up the opportunity to analyze many different biomolecules, both lipids and proteins, at the same time, with high spatial resolution. The method has been applied to detect and image the distribution of amyloid-β (Aβ), a biologically relevant peptide in Alzheimer’s disease (AD), in transgenic mouse brain tissue. To ensure specific binding, the immunoliposome binding was verified on a model surface using quartz crystal microbalance with dissipation monitoring. The immunoliposome binding was also investigated on tissue sections with fluorescence microscopy, and compared with conventional immunohistochemistry using primary and secondary antibodies, demonstrating specific binding to Aβ. Using ToF-SIMS imaging, several endogenous lipids, such as cholesterol and sulfatides, were also detected in parallel with the immunoliposome-labeled Aβ deposits, which is an advantage compared to fluorescence microscopy. This method can thus potentially provide further information about lipid–protein interactions, which is important to understand the mechanisms of neurodegeneration in AD.

  • 50.
    Carlsson, B
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Möller, K et al
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Qualification test procedure for solar absorber surface durability1999In: Solar Energy Materials and Solar Cells, ISSN 0927-0248, E-ISSN 1879-3398, Vol. 61, no 3, p. 255-275Article in journal (Other academic)
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