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  • 1.
    Fu, Ying
    et al.
    KTH Royal Institute of Technology, Sweden.
    Jussi, Johnny
    RISE - Research Institutes of Sweden, ICT, Acreo. KTH Royal Institute of Technology, Sweden.
    Elmlund, Louise
    Swedish National Forensic Centre, Sweden.
    Dunne, Simon
    Swedish National Forensic Centre, Sweden.
    Wang, Qin
    RISE - Research Institutes of Sweden, ICT, Acreo.
    Brismar, Hjalmar
    KTH Royal Institute of Technology, Sweden.
    Intrinsic blinking characteristics of single colloidal CdSe-CdS/ZnS core-multishell quantum dots2019Inngår i: Physical Review B, ISSN 2469-9950, E-ISSN 2469-9969, Vol. 99, nr 3, artikkel-id 035404Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Fluorescence blinking of single colloidal semiconductor quantum dots (QDs) has been extensively studied, and several sophisticated models have been proposed. In this work, we derive Heisenberg equations of motion to carefully study principal transition processes, i.e., photoexcitation, energy relaxation, impact ionization and Auger recombination, radiative and nonradiative recombinations, and tunneling between core states and surface states, of the electron-hole pair in single CdSe-CdS/ZnS core-multishell QDs and show that the on-state probability density distribution of the QD fluorescence obeys the random telegraph signal theory because of the random radiative recombination of the photoexcited electron-hole pair in the QD core, while the off-state probability density distribution obeys the inverse power law distribution due to the series of random walks of the photoexcited electron in the two-dimensional surface-state network after the electron tunnels from the QD core to the QD surface. These two different blinking characteristics of the single QD are resolved experimentally by properly adjusting the optical excitation power and the bin time.

  • 2.
    Fu, Ying
    et al.
    KTH Royal Institute of Technology, Sweden.
    Jussi, Johnny
    RISE - Research Institutes of Sweden, ICT, Acreo. KTH Royal Institute of Technology, Sweden.
    Wang, Qin
    RISE - Research Institutes of Sweden, ICT, Acreo.
    Brismar, Hjalmar
    KTH Royal Institute of Technology, Sweden.
    Liu, Yushen
    College of Physics and Electronic Engineering, China.
    Yang, Xifeng
    College of Physics and Electronic Engineering, China.
    Chen, Yun
    University of Gothenburg, Sweden.
    Endocytic pathway of vascular cell adhesion molecule 1 in human umbilical vein endothelial cell identified in vitro by using functionalized nontoxic fluorescent quantum dots2019Inngår i: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 297, artikkel-id 126702Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Studies about vascular cell adhesion molecule 1 (VCAM1) in tumor growth, metastasis, and angiogenesis suggest that targeting VCAM1 expression is an attractive strategy for diagnosis and anti-tumor therapy. However, the endocytic pathway of VCAM1 in vascular cells has not been well characterized. In this study we visualize the endocytic pathway of tumor necrosis factor α (TNFα) induced VCAM1 in human umbilical vein endothelial cell (HUVEC) in vitro using 5-carboxyfluorescein labeled VCAM1 binding peptides and fluorescent water-dispersible 3-mercaptopropionic acid (3MPA)-coated CdSe-CdS/Cd0.5Zn0.5S/ZnS core–multishell nontoxic quantum dots (3MPA-QDs) functionalized with VCAM1 binding peptides. Clear key in vitro observations are as follows: (a) 3MPA-QDs functionalized with VCAM1 binding peptides, denoted as VQDs, adhered and aggregated cumulatively to cell membrane around 2 h after VQD deposition to cell culture medium and were found in lysosomes in TNFα-treated HUVECs approximately 24 h after VQD deposition; (b) VQDs remained in TNFα-treated HUVECs for the whole 16 days of the experimental observation period; (c) quite differently, 3MPA-QDs were endocytosed then exocytosed by HUVECs via endosomes in about 24–48 h after 3MPA-QD deposition. Our study suggests that VCAM1 molecules, initially expressed on cell membrane induced by TNFα treatment, are internalized into lysosomes. This provides a novel means to deliver materials to lysosomes such as enzyme replacement therapy. Moreover, our meticulous sensing methodology of devising fluorescent nontoxic QDs advances biosensing technique for studying cellular activities in vitro and in vivo. © 2019 The Authors

  • 3.
    Karlsson, Mikael
    et al.
    RISE - Research Institutes of Sweden, ICT, Acreo. Pamitus AB, Sweden.
    Strandqvist, Carl
    Swedish National Forensic Centre, Sweden.
    Jussi, Johnny
    RISE - Research Institutes of Sweden, ICT, Acreo. KTH Royal Institute of Technology, Sweden.
    Öberg, Olof
    RISE - Research Institutes of Sweden, ICT, Acreo.
    Petermann, Ingemar
    RISE - Research Institutes of Sweden, ICT, Acreo.
    Elmlund, Louise
    Swedish National Forensic Centre, Sweden.
    Dunne, Simon
    Swedish National Forensic Centre, Sweden.
    Fu, Ying
    KTH Royal Institute of Technology, Sweden.
    Wang, Qin
    RISE - Research Institutes of Sweden, ICT, Acreo.
    Chemical Sensors Generated on Wafer-Scale Epitaxial Graphene for Application to Front-Line Drug Detection2019Inngår i: Sensors, ISSN 1424-8220, E-ISSN 1424-8220, Vol. 19, nr 10, artikkel-id 2214Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Generation of large areas of graphene possessing high quality and uniformity will be a critical factor if graphene-based devices/sensors are to be commercialized. In this work, epitaxial graphene on a 2" SiC wafer was used to fabricate sensors for the detection of illicit drugs (amphetamine or cocaine). The main target application is on-site forensic detection where there is a high demand for reliable and cost-efficient tools. The sensors were designed and processed with specially configured metal electrodes on the graphene surface by utilizing a series of anchors where the metal contacts are directly connected on the SiC substrate. This has been shown to improve adhesion of the electrodes and decrease the contact resistance. A microfluidic system was constructed to pump solutions over the defined graphene surface that could then act as a sensor area and react with the target drugs. Several prototypic systems were tested where non-covalent interactions were used to localize the sensing components (antibodies) within the measurement cell. The serendipitous discovery of a wavelength-dependent photoactivity for amphetamine and a range of its chemical analogs, however, limited the general application of these prototypic systems. The experimental results reveal that the drug molecules interact with the graphene in a molecule dependent manner based upon a balance of π -stacking interaction of the phenyl ring with graphene (p-doping) and the donation of the amine nitrogens lone pair electrons into the π - π *-system of graphene (n-doping).

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