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  • 1.
    Dahlbom, Sixten
    et al.
    RISE Research Institutes of Sweden, Säkerhet och transport, Brand och Säkerhet.
    Bjarnemark, Fanny
    RISE Research Institutes of Sweden, Material och produktion, Kemi och Tillämpad mekanik.
    Nguyen, Björn
    RISE Research Institutes of Sweden, Material och produktion, Kemi och Tillämpad mekanik.
    Petronis, Sarunas
    RISE Research Institutes of Sweden, Material och produktion, Metodik för produktframtagning.
    Mallin, Tove
    RISE Research Institutes of Sweden, Säkerhet och transport, Brand och Säkerhet.
    Analysis of per- and polyfluoroalkyl substances (PFAS) extraction from contaminated firefighting materials: Effects of cleaning agent, temperature, and chain-length dependencies2024Ingår i: Emerging Contaminants, ISSN 2405-6650, E-ISSN 2405-6642, Vol. 10, nr 3, s. 100335-100335, artikel-id 100335Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    This investigation delves into the extraction dynamics of 22 per- and polyfluoroalkyl substances from PFAS contaminated firefighting materials. Two distinct test sets were executed: one contrasting a commercial product with water following an elaborate decontamination procedure, and the other assessing seven washing agents on materials from firefighting installations, with one agent examined at 22 °C and 50 °C. A general tendency for improved desorption at the higher temperature was observed. Furthermore, a discernible influence of the cleaning agent's pH on the extraction of specific PFAS species was observed, elucidating the role of chemical environment in the extraction process. PFAS rebound was studied for a period of up to 157 days, this unveiled a gradual escalation in PFAS22 levels, indicative of a protracted desorption mechanism. Intriguingly, PFAS with abbreviated carbon chains (C4–C6) exhibit superior desorption efficiency compared to their elongated congeners, suggesting a chain-length-dependent decontamination potential. A comparative scrutiny between a commercially available cleaning product, featuring multiple washing and flushing steps, and a water-only treatment regimen underscores the potential efficacy of the former. This exhaustive investigation furnishes nuanced insights into PFAS extraction complexities, offering a foundation for informed decontamination strategies

    Ladda ner fulltext (pdf)
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  • 2.
    de Peppo, Giuseppe Maria
    et al.
    The New York Stem Cell Foundation Research Institute, USA; Sahlgrenska Academy, Sweden; BIOMATCELL, Sweden.
    Agheli, Hossein
    Sahlgrenska Academy, Sweden; BIOMATCELL, Sweden.
    Karlsson, Camilla
    Sahlgrenska Academy, Sweden; BIOMATCELL, Sweden.
    Ekström, Karin
    Sahlgrenska Academy, Sweden; BIOMATCELL, Sweden.
    Brisby, Helena
    Sahlgrenska Academy, Sweden; BIOMATCELL, Sweden.
    Lennerås, Maria E.
    Sahlgrenska Academy, Sweden; BIOMATCELL, Sweden.
    Gustafsson, Stefan
    BIOMATCELL, Sweden; Chalmers University of Technology, Sweden.
    Sjövall, Peter
    RISE., SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SP – Sveriges Tekniska Forskningsinstitut / Funktionella material (KMf).
    Johansson, Anna Karin
    Sahlgrenska Academy, Sweden; BIOMATCELL, Sweden.
    Olsson, Eva
    BIOMATCELL, Sweden; Chalmers University of Technology, Sweden.
    Lausmaa, Jukka
    RISE., SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SP – Sveriges Tekniska Forskningsinstitut / Funktionella material (KMf).
    Thomsen, Peter
    Sahlgrenska Academy, Sweden; BIOMATCELL, Sweden.
    Petronis, Sarunas
    RISE., SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. BIOMATCELL, Sweden.
    Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro2014Ingår i: International Journal of Nanomedicine, ISSN 1176-9114, E-ISSN 1178-2013, Vol. 9, nr 1, s. 2499-2515Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Background: Patterning medical devices at the nanoscale level enables the manipulation of cell behavior and tissue regeneration, with topographic features recognized as playing a significant role inthe osseointegration of implantable devices. Methods: In this study, we assessed the ability of titanium-coated hemisphere-like topographic nanostructures of different sizes (approximately 50, 100, and 200 nm) to influence the morphology, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs). Results: We found that the proliferation and osteogenicdifferentiation of hMSCs was influenced by the size of the underlying structures, suggesting that size variations in topographic features at the nanoscale level, independently of chemistry, can be exploited to control hMSC behavior in a size-dependent fashion. Conclusion: Our studies demonstrate that colloidal lithography, in combination with coating technologies, can be exploited to investigate the cell response to well defined nanoscale topography and to develop next-generation surfaces that guide tissue regeneration and promote implant integration.

  • 3.
    Flys, Olena
    et al.
    RISE - Research Institutes of Sweden (2017-2019), Säkerhet och transport, Mätteknik. Halmstad University, Sweden.
    Jarlemark, Per
    RISE - Research Institutes of Sweden (2017-2019), Säkerhet och transport, Mätteknik.
    Petronis, Sarunas
    RISE - Research Institutes of Sweden (2017-2019), Biovetenskap och material, Kemi och material.
    Stenlund, Patrik
    RISE - Research Institutes of Sweden (2017-2019), Biovetenskap och material, Kemi och material.
    Rosen, B. -G
    Halmstad University, Sweden.
    Applicability of characterization techniques on fine scale surfaces2018Ingår i: Surface Topography: Metrology and Properties, ISSN 2051-672X, Vol. 6, nr 3, artikel-id 034015Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    In this study, several surface topographies typical for dental implants were evaluated by different measurement techniques. The samples were prepared by machine turning, wet chemical etching and electrochemical polishing of titanium discs. The measurement techniques included an atomic force microscope (AFM), coherence scanning interferometer (CSI) and a 3D stereo scanning electron microscope (SEM). The aim was to demonstrate and discuss similarities and differences in the results provided by these techniques when analyzing submicron surface topographies. The estimated surface roughness parameters were not directly comparable since the techniques had different surface spatial wavelength band limits. However, the comparison was made possible by applying a 2D power spectral density (PSD) function. Furthermore, to simplify the comparison, all measurements were characterized using the ISO 25178 standard parameters. Additionally, a Fourier transform was applied to calculate the instrument transfer function in order to investigate the behavior of CSI at different wavelength ranges. The study showed that 3D stereo SEM results agreed well with AFM measurements for the studied surfaces. Analyzed surface parameter values were in general higher when measured by CSI in comparison to both AFM and 3D stereo SEM results. In addition, the PSD analysis showed a higher power spectrum density in the lower frequency range 10-2-10-1 μm-1 for the CSI compared with the other techniques.

  • 4.
    Granskog, Viktor
    et al.
    KTH Royal Institute of Technology, Sweden.
    García-Gallego, Sandra
    KTH Royal Institute of Technology, Sweden.
    von Kieseritzky, Johanna
    Karolinska Institutet, Sweden.
    Rosendahl, Jennifer
    RISE - Research Institutes of Sweden (2017-2019), Biovetenskap och material, Kemi och material.
    Stenlund, Patrik
    RISE - Research Institutes of Sweden (2017-2019), Biovetenskap och material, Kemi och material.
    Zhang, Yuning
    KTH Royal Institute of Technology, Sweden.
    Petronis, Sarunas
    RISE - Research Institutes of Sweden (2017-2019), Biovetenskap och material, Kemi och material.
    Lyvén, Benny
    RISE - Research Institutes of Sweden (2017-2019), Biovetenskap och material, Kemi och material.
    Arner, Marianne
    Karolinska Institutet, Sweden.
    Håkansson, Joakim
    RISE - Research Institutes of Sweden (2017-2019), Biovetenskap och material, Kemi och material.
    Malkoch, Michael
    KTH Royal Institute of Technology, Sweden.
    High-Performance Thiol–Ene Composites Unveil a New Era of Adhesives Suited for Bone Repair2018Ingår i: Advanced Functional Materials, ISSN 1616-301X, E-ISSN 1616-3028, Vol. 28, nr 26, artikel-id 1800372Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The use of adhesives for fracture fixation can revolutionize the surgical procedures toward more personalized bone repairs. However, there are still no commercially available adhesive solutions mainly due to the lack of biocompatibility, poor adhesive strength, or inadequate fixation protocols. Here, a surgically realizable adhesive system capitalizing on visible light thiol–ene coupling chemistry is presented. The adhesives are carefully designed and formulated from a novel class of chemical constituents influenced by dental resin composites and self-etch primers. Validation of the adhesive strength is conducted on wet bone substrates and accomplished via fiber-reinforced adhesive patch (FRAP) methodology. The results unravel, for the first time, on the promise of a thiol–ene adhesive with an unprecedented shear bond strength of 9.0 MPa and that surpasses, by 55%, the commercially available acrylate dental adhesive system Clearfil SE Bond of 5.8 MPa. Preclinical validation of FRAPs on rat femur fracture models details good adhesion to the bone throughout the healing process, and are found biocompatible not giving rise to any inflammatory response. Remarkably, the FRAPs are found to withstand loads up to 70 N for 1000 cycles on porcine metacarpal fractures outperforming clinically used K-wires and match metal plates and screw implants.

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  • 5.
    Håkansson, Joakim
    et al.
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil. University of Gothenburg, Sweden.
    Simsa, Robin
    VERIGRAFT AB, Sweden.
    Bogestål, Yalda
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Jenndahl, Lachmi
    VERIGRAFT AB, Sweden.
    Gustafsson-Hedberg, Tobias
    VERIGRAFT AB, Sweden.
    Petronis, Sarunas
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Strehl, Raimund
    VERIGRAFT AB, Sweden.
    Österberg, Klas
    Sahlgrenska Academy, Sweden.
    Individualized tissue-engineered veins as vascular grafts: a proof of concept study in pig.2021Ingår i: Journal of Tissue Engineering and Regenerative Medicine, ISSN 1932-6254, E-ISSN 1932-7005, Vol. 15, nr 10, s. 818-Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Personalized tissue engineered vascular grafts are a promising advanced therapy medicinal product (ATMP) alternative to autologous or synthetic vascular grafts utilized in blood vessel bypass or replacement surgery. We hypothesized that an individualized tissue engineered vein (P-TEV) would make the body recognize the transplanted blood vessel as autologous, decrease the risk of rejection and thereby avoid lifelong treatment with immune suppressant medication as is standard with allogenic organ transplantation. To individualize blood vessels, we decellularized vena cava from six deceased donor pigs and tested them for cellular removal and histological integrity. A solution with peripheral blood from the recipient pigs was used for individualized reconditioning in a perfusion bioreactor for seven days prior to transplantation. To evaluate safety and functionality of the individualized vascular graft in vivo, we transplanted reconditioned porcine vena cava into six pigs and analyzed histology and patency of the graft at different time points, with three pigs at the final endpoint 4-5 weeks after surgery. Our results showed that the P-TEV was fully patent in all animals, did not induce any occlusion or stenosis formation and we did not find any signs of rejection. The P-TEV showed rapid recellularization in vivo with the luminal surface covered with endothelial cells. In summary, the results indicate that P-TEV is functional and have potential for use as clinical transplant grafts. 

  • 6.
    Jenndahl, L.
    et al.
    VERIGRAFT AB, Sweden.
    Österberg, K.
    Sahlgrenska Academy, Sweden.
    Bogestål, Yalda
    RISE Research Institutes of Sweden, Material och produktion, Metodik för produktframtagning.
    Simsa, R.
    VERIGRAFT AB, Sweden; University of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden.
    Gustafsson-Hedberg, T.
    VERIGRAFT AB, Sweden.
    Stenlund, Patrik
    RISE Research Institutes of Sweden, Material och produktion, Metodik för produktframtagning.
    Petronis, Sarunas
    RISE Research Institutes of Sweden, Material och produktion, Metodik för produktframtagning.
    Krona, Annika
    RISE Research Institutes of Sweden, Bioekonomi och hälsa, Jordbruk och livsmedel.
    Fogelstrand, P.
    University of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden.
    Strehl, R.
    VERIGRAFT AB, Sweden.
    Håkansson, Joakim
    RISE Research Institutes of Sweden, Material och produktion, Metodik för produktframtagning. University of Gothenburg, Sweden.
    Personalized tissue-engineered arteries as vascular graft transplants: A safety study in sheep2022Ingår i: Regenerative Therapy, ISSN 2352-3204, Vol. 21, s. 331-341Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Patients with cardiovascular disease often need replacement or bypass of a diseased blood vessel. With disadvantages of both autologous blood vessels and synthetic grafts, tissue engineering is emerging as a promising alternative of advanced therapy medicinal products for individualized blood vessels. By reconditioning of a decellularized blood vessel with the recipient's own peripheral blood, we have been able to prevent rejection without using immunosuppressants and prime grafts for efficient recellularization in vivo. Recently, decellularized veins reconditioned with autologous peripheral blood were shown to be safe and functional in a porcine in vivo study as a potential alternative for vein grafting. In this study, personalized tissue engineered arteries (P-TEA) were developed using the same methodology and evaluated for safety in a sheep in vivo model of carotid artery transplantation. Five personalized arteries were transplanted to carotid arteries and analyzed for safety and patency as well as with histology after four months in vivo. All grafts were fully patent without any occlusion or stenosis. The tissue was well cellularized with a continuous endothelial cell layer covering the luminal surface, revascularized adventitia with capillaries and no sign of rejection or infection. In summary, the results indicate that P-TEA is safe to use and has potential as clinical grafts. 

  • 7.
    Karazisis, Dimitrios
    et al.
    University of Gothenburg, Sweden.
    Ballo, Ahmed M.
    University of Gothenburg, Sweden; University of British Columbia, Canada.
    Petronis, Sarunas
    RISE., SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. University of Gothenburg, Sweden.
    Agheli, Hossein
    University of Gothenburg, Sweden.
    Emanuelsson, Lena
    University of Gothenburg, Sweden.
    Thomsen, Peter
    University of Gothenburg, Sweden.
    Omar, Omar
    University of Gothenburg, Sweden.
    The role of well-defined nanotopography of titanium implants on osseointegration: Cellular and molecular events in vivo2016Ingår i: International Journal of Nanomedicine, ISSN 1176-9114, E-ISSN 1178-2013, Vol. 11, s. 1367-1382Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Purpose: Mechanisms governing the cellular interactions with well-defined nanotopography are not well described in vivo. This is partly due to the difficulty in isolating a particular effect of nanotopography from other surface properties. This study employed colloidal lithography for nanofabrication on titanium implants in combination with an in vivo sampling procedure and different analytical techniques. The aim was to elucidate the effect of well-defined nanotopography on the molecular, cellular, and structural events of osseointegration. Materials and methods: Titanium implants were nanopatterned (Nano) with semispherical protrusions using colloidal lithography. Implants, with and without nanotopography, were implanted in rat tibia and retrieved after 3, 6, and 28 days. Retrieved implants were evaluated using quantitative polymerase chain reaction, histology, immunohistochemistry, and energy dispersive X-ray spectroscopy (EDS). Results: Surface characterization showed that the nanotopography was well defined in terms of shape (semispherical), size (79±6 nm), and distribution (31±2 particles/μm2). EDS showed similar levels of titanium, oxygen, and carbon for test and control implants, confirming similar chemistry. The molecular analysis of the retrieved implants revealed that the expression levels of the inflammatory cytokine, TNF-α, and the osteoclastic marker, CatK, were reduced in cells adherent to the Nano implants. This was consistent with the observation of less CD163-positive macrophages in the tissue surrounding the Nano implant. Furthermore, periostin immunostaining was frequently detected around the Nano implant, indicating higher osteogenic activity. This was supported by the EDS analysis of the retrieved implants showing higher content of calcium and phosphate on the Nano implants. Conclusion: The results show that Nano implants elicit less periimplant macrophage infiltration and downregulate the early expression of inflammatory (TNF-α) and osteoclastic (CatK) genes. Immunostaining and elemental analyses show higher osteogenic activity at the Nano implant. It is concluded that an implant with the present range of well-defined nanocues attenuates the inflammatory response while enhancing mineralization during osseointegration.

  • 8.
    Karazisis, Dimitrios
    et al.
    University of Gothenburg, Sweden.
    Omar, Omar
    Imam Abdulrahman Bin Faisal University, Saudi Arabia.
    Petronis, Sarunas
    RISE Research Institutes of Sweden, Material och produktion, Metodik för produktframtagning.
    Thomsen, Peter
    University of Gothenburg, Sweden.
    Rasmusson, Lars
    University of Gothenburg, Sweden; Linköping University Hospital, Sweden.
    Molecular Response to Nanopatterned Implants in the Human Jaw Bone2021Ingår i: ACS Biomaterials Science & Engineering, E-ISSN 2373-9878, Vol. 7, nr 12, s. 5878-5889Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Implant surface modification by nanopatterning is an interesting route for enhancing osseointegration in humans. Herein, the mol. response to an intentional, controlled nanotopog. pattern superimposed on screw-shaped titanium implants is investigated in human bone. When clin. implants are installed, addnl. two mini-implants, one with a machined surface (M) and one with a machined surface superimposed with a hemispherical nanopattern (MN), are installed in the posterior maxilla. In the second-stage surgery, after 6-8 wk, the mini-implants are retrieved by unscrewing, and the implant-adherent cells are subjected to gene expression anal. using quant. polymerase chain reaction (qPCR). Compared to those adherent to the machined (M) implants, the cells adherent to the nanopatterned (MN) implants demonstrate significant upregulation (1.8- to 2-fold) of bone-related genes (RUNX2, ALP, and OC). No significant differences are observed in the expression of the analyzed inflammatory and remodeling genes. Correlation anal. reveals that older patient age is associated with increased expression of proinflammatory cytokines (TNF-α and MCP-1) on the machined implants and decreased expression of pro-osteogenic factor (BMP-2) on the nanopatterned implants. Controlled nanotopog., in the form of hemispherical 60 nm protrusions, promotes gene expressions related to early osteogenic differentiation and osteoblastic activity in implant-adherent cells in the human jaw bone.

  • 9.
    Karazisis, Dimitrios
    et al.
    Sahlgrenska Academy, Sweden; University of Gothenburg, Sweden; BIOMATCELL, Sweden.
    Petronis, Sarunas
    RISE - Research Institutes of Sweden (2017-2019), Biovetenskap och material, Kemi och material. BIOMATCELL, Sweden.
    Agheli, Hossein
    Sahlgrenska Academy, Sweden; University of Gothenburg, Sweden; BIOMATCELL, Sweden.
    Emanuelsson, Lena
    Sahlgrenska Academy, Sweden; University of Gothenburg, Sweden; BIOMATCELL, Sweden.
    Norlindh, Birgitta
    Sahlgrenska Academy, Sweden; University of Gothenburg, Sweden; BIOMATCELL, Sweden.
    Johansson, Anna
    Sahlgrenska Academy, Sweden; University of Gothenburg, Sweden; BIOMATCELL, Sweden.
    Rasmusson, Lars
    Sahlgrenska Academy, Sweden; University of Gothenburg, Sweden; BIOMATCELL, Sweden.
    Thomsen, Peter
    Sahlgrenska Academy, Sweden; University of Gothenburg, Sweden; BIOMATCELL, Sweden.
    Omar, Omar
    Sahlgrenska Academy, Sweden; University of Gothenburg, Sweden; BIOMATCELL, Sweden.
    The influence of controlled surface nanotopography on the early biological events of osseointegration.2017Ingår i: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 53, s. 559-571Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The early cell and tissue interactions with nanopatterned titanium implants are insufficiently described in vivo. A limitation has been to transfer a pre-determined, well-controlled nanotopography to 3D titanium implants, without affecting other surface parameters, including surface microtopography and chemistry. This in vivo study aimed to investigate the early cellular and molecular events at the bone interface with screw-shaped titanium implants superimposed with controlled nanotopography. Polished and machined titanium implants were firstly patterned with 75-nm semispherical protrusions. Polished and machined implants without nano-patterns were designated as controls. Thereafter, all nanopatterned and control implants were sputter-coated with a 30nm titanium layer to unify the surface chemistry. The implants were inserted in rat tibiae and samples were harvested after 12h, 1d and 3d. In one group, the implants were unscrewed and the implant-adherent cells were analyzed using quantitative polymerase chain reaction. In another group, implants with surrounding bone were harvested en bloc for histology and immunohistochemistry. The results showed that nanotopography downregulated the expression of monocyte chemoattractant protein-1 (MCP-1), at 1d, and triggered the expression of osteocalcin (OC) at 3d. This was in parallel with a relatively lower number of recruited CD68-positive macrophages in the tissue surrounding the nanopatterned implants. Moreover, a higher proportion of newly formed osteoid and woven bone was found at the nanopatterned implants at 3d. It is concluded that nanotopography, per se, attenuates the inflammatory process and enhances the osteogenic response during the early phase of osseointegration. This nanotopography-induced effect appeared to be independent of the underlying microscale topography.

    STATEMENT OF SIGNIFICANCE: This study provides a first line of evidence that pre-determined nanopatterns on clinically relevant, screw-shaped, titanium implants can be recognized by cells in the complex in vivo environment. Until now, most of the knowledge relating to cell interactions with nanopatterned surfaces has been acquired from in vitro studies involving mostly two-dimensional nanopatterned surfaces of varying chemical composition. We have managed to superimpose pre-determined nanoscale topography on polished and micro-rough, screw-shaped, implants, without changes in the microscale topography or chemistry. This was achieved by colloidal lithography in combination with a thin titanium film coating on top of both nanopatterned and control implants. The early events of osseointegration were evaluated at the bone interface to these implants. The results revealed that nanotopography, as such, elicits downregulatory effects on the early recruitment and activity of inflammatory cells while enhancing osteogenic activity and woven bone formation.

  • 10.
    Karazisis, Dimitrios
    et al.
    University of Gothenburg, Sweden.
    Rasmusson, Lars
    University of Gothenburg, Sweden; Linköping University Hospital, Sweden.
    Petronis, Sarunas
    RISE Research Institutes of Sweden, Material och produktion, Metodik för produktframtagning.
    Palmquist, Anders
    University of Gothenburg, Sweden.
    Shah, Furqan
    University of Gothenburg, Sweden.
    Agheli, Hossein
    University of Gothenburg, Sweden.
    Emanuelsson, Lena
    University of Gothenburg, Sweden.
    Johansson, Anna
    University of Gothenburg, Sweden.
    Omar, Omar
    Imam Abdulrahman bin Faisal University, Saudi Arabia.
    Thomsen, Peter
    University of Gothenburg, Sweden.
    The effects of controlled nanotopography, machined topography and their combination on molecular activities, bone formation and biomechanical stability during osseointegration2021Ingår i: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 136, s. 279-290Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The initial cellular and molecular activities at the bone interface of implants with controlled nanoscale topography and microscale roughness have previously been reported. However, the effects of such surface modifications on the development of osseointegration have not yet been determined. This study investigated the molecular events and the histological and biomechanical development of the bone interface in implants with nanoscale topography, microscale roughness or a combination of both. Polished and machined titanium implants with and without controlled nanopatterning (75 nm protrusions) were produced using colloidal lithography and coated with a thin titanium layer to unify the chemistry. The implants were inserted in rat tibiae and subjected to removal torque (RTQ) measurements, molecular analyses and histological analyses after 6, 21 and 28 days. The results showed that nanotopography superimposed on microrough, machined, surfaces promoted an early increase in RTQ and hence produced greater implant stability at 6 and 21 days. Two-way MANOVA revealed that the increased RTQ was influenced by microscale roughness and the combination of nanoscale and microscale topographies. Furthermore, increased bone-implant contact (BIC) was observed with the combined nanopatterned machined surface, although MANOVA results implied that the increased BIC was mainly dependent on microscale roughness. At the molecular level, the nanotopography, per se, and in synergy with microscale roughness, downregulated the expression of the proinflammatory cytokine tumor necrosis factor alpha (TNF-α). In conclusion, controlled nanotopography superimposed on microrough machined implants promoted implant stability during osseointegration. Nanoscale-driven mechanisms may involve attenuation of the inflammatory response at the titanium implant site. Statement of Significance: The role of combined implant microscale and nanotopography features for osseointegration is incompletely understood. Using colloidal lithography technique, we created an ordered nanotopography pattern superimposed on screwshaped implants with microscale topography. The midterm and late molecular, bone-implant contact and removal torque responses were analysed in vivo. Nanotopography superimposed on microrough, machined, surfaces promoted the implant stability, influenced by microscale topography and the combination of nanoscale and microscale topographies. Increased bone-implant contact was mainly dependent on microscale roughness whereas the nanotopography, per se, and in synergy with microscale roughness, attenuated the proinflammatory tumor necrosis factor alpha (TNF-α) expression. It is concluded that microscale and nanopatterns provide individual as well as synergistic effects on molecular, morphological and biomechanical implant-tissue processes in vivo. © 2021 The Author(s)

  • 11.
    Knutsen, Maja
    et al.
    Oxy Solutions, Norway.
    Agrenius, Karin
    RISE Research Institutes of Sweden, Material och produktion, Metodik för produktframtagning.
    Ugland, Hege
    Petronis, Sarunas
    RISE Research Institutes of Sweden, Material och produktion, Metodik för produktframtagning.
    Haglerod, Camilla
    Oxy Solutions, Norway.
    Håkansson, Joakim
    RISE Research Institutes of Sweden, Material och produktion, Metodik för produktframtagning. Gothenburg University, Sweden.
    Chinga-Carrasco, Gary
    RISE Research Institutes of Sweden, Bioekonomi och hälsa, Material- och ytdesign.
    Oxygenated Nanocellulose - A Material Platform for Antibacterial Wound Dressing Devices2021Ingår i: ACS Applied Bio Materials, E-ISSN 2576-6422, Vol. 4, nr 10, s. 7554-7562Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Both carboxylated cellulose nanofibrils (CNF) and dissolved oxygen (DO) have been reported to possess antibacterial properties. However, the combination for use as wound dressings against biofilm infections in chronic wounds is less known. The present study reports the development of oxygenated CNF dispersions that exhibit strong antibacterial effect. Carboxylated CNF dispersions with different oxidation levels were oxygenated by the OXY BIO System and tested for antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus. The results reveal that the higher oxidation level of the CNFs, the better antibacterial effect. Scanning electron microscopy of bacterial biofilms revealed that a potential mechanism of action of the CNFs is the formation of a network surrounding and entrapping the bacteria. This effect is further potentiated by the oxygenation process. A CNF sample (concentration 0.6 wt %) that was oxygenated to a DO level of 46.4 mg/L demonstrated a strong antibacterial effect against S. aureus in vivo using a mouse model of surgical site infection. The oxygenated CNF dispersion reduced the bacterial survival by 71%, after 24 h treatment. The potent antibacterial effect indicates that oxygenated nanocellulose is a promising material for antibacterial wound dressings. © 2021 The Authors.

  • 12.
    Kuna, V K
    et al.
    University of Gothenburg, Sweden.
    Padma, A M
    University of Gothenburg, Sweden.
    Håkansson, Joakim
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Nygren, J
    TATAA Biocenter, Sweden.
    Sjöback, R
    TATAA Biocenter, Sweden.
    Petronis, Sarunas
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Sumitran-Holgersson, S
    University of Gothenburg, Sweden.
    Significantly accelerated wound healing of full-thickness skin using a novel composite gel of porcine acellular dermal matrix and human peripheral blood cells2017Ingår i: Cell Transplantation, ISSN 0963-6897, E-ISSN 1555-3892, Vol. 26, nr 2, s. 293-307Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Herein, we report the fabrication of a novel composite gel from decellularized gal-gal-knockout porcine skin and human peripheral blood mononuclear cells (hPBMC) for full-thickness skin wound healing. Decellularized skin extracellular matrix (ECM) powder was prepared via chemical treatment, freeze-drying and homogenization. The powder was mixed with culture medium containing hyaluronic acid to generate a pig skin gel (PSG). The effect of the gel in regeneration of full-thickness wound was studied in nude mice. We found significantly accelerated wound closure already on day 15 in animals treated with PSG only or PSG+hPBMC as compared to untreated and hyaluronic acid treated controls (p<0.05). Addition of the hPBMC to the gel resulted in marked increase of host blood vessels as well as the presence of human blood vessels. At day 25, histologically, the wounds in animals treated with PSG only or PSG+hPBMC were completely closed as compared to controls. Thus, the gel facilitated generation of new skin with well arranged epidermal cells and restored bilayer structure of the epidermis and dermis. These results suggest that porcine skin ECM gel together with human cells may be a novel and promising biomaterial for medical applications especially for patients with acute and chronic skin wounds.

  • 13.
    Landberg, G.
    et al.
    University of Gothenburg, Sweden.
    Jonasson, E.
    University of Gothenburg, Sweden.
    Gustafsson, A.
    University of Gothenburg, Sweden.
    Fitzpatrick, P.
    University of Gothenburg, Sweden.
    Isakson, P.
    University of Gothenburg, Sweden.
    Karlsson, J.
    University of Gothenburg, Sweden.
    Larsson, E.
    University of Gothenburg, Sweden.
    Svanström, A.
    University of Gothenburg, Sweden.
    Rafnsdottir, S.
    University of Gothenburg, Sweden.
    Persson, E.
    University of Gothenburg, Sweden.
    Andersson, D.
    University of Gothenburg, Sweden.
    Rosendahl, Jennifer
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Petronis, Sarunas
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Ranji, P.
    University of Gothenburg, Sweden.
    Gregersson, P.
    University of Gothenburg, Sweden.
    Magnusson, Y.
    University of Gothenburg, Sweden.
    Håkansson, Joakim
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Ståhlberg, A.
    University of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden.
    Characterization of cell-free breast cancer patient-derived scaffolds using liquid chromatography-mass spectrometry/mass spectrometry data and RNA sequencing data2020Ingår i: Data in Brief, E-ISSN 2352-3409, Vol. 31, artikel-id 105860Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Patient-derived scaffolds (PDSs) generated from primary breast cancer tumors can be used to model the tumor microenvironment in vitro. Patient-derived scaffolds are generated by repeated detergent washing, removing all cells. Here, we analyzed the protein composition of 15 decellularized PDSs using liquid chromatography-mass spectrometry/mass spectrometry. One hundred forty-three proteins were detected and their relative abundance was calculated using a reference sample generated from all PDSs. We performed heatmap analysis of all the detected proteins to display their expression patterns across different PDSs together with pathway enrichment analysis to reveal which processes that were connected to PDS protein composition. This protein dataset together with clinical information is useful to investigators studying the microenvironment of breast cancers. Further, after repopulating PDSs with either MCF7 or MDA-MB-231 cells, we quantified their gene expression profiles using RNA sequencing. These data were also compared to cells cultured in conventional 2D conditions, as well as to cells cultured as xenografts in immune-deficient mice. We investigated the overlap of genes regulated between these different culture conditions and performed pathway enrichment analysis of genes regulated by both PDS and xenograft cultures compared to 2D in both cell lines to describe common processes associated with both culture conditions. Apart from our described analyses of these systems, these data are useful when comparing different experimental model systems. Downstream data analyses and interpretations can be found in the research article “Patient-derived scaffolds uncover breast cancer promoting properties of the microenvironment” [1]. © 2020 The Authors

  • 14.
    Landberg, Göran
    et al.
    University of Gothenburg, Sweden.
    Fitzpatrick, Paul
    University of Gothenburg, Sweden.
    Isakson, Pauline
    University of Gothenburg, Sweden.
    Jonasson, Emma
    University of Gothenburg, Sweden.
    Karlsson, Joakim
    University of Gothenburg, Sweden.
    Larsson, Erik
    University of Gothenburg, Sweden.
    Svanström, Andreas
    University of Gothenburg, Sweden.
    Rafnsdottir, Svanheidur
    University of Gothenburg, Sweden.
    Persson, Emma
    University of Gothenburg, Sweden.
    Gustafsson, Annna
    University of Gothenburg, Sweden.
    Andersson, Daniel
    University of Gothenburg, Sweden.
    Rosendahl, Jennifer
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Petronis, Sarunas
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Ranji, Parmida
    University of Gothenburg, Sweden.
    Gregersson, Pernilla
    University of Gothenburg, Sweden.
    Magnusson, Ylva
    University of Gothenburg, Sweden.
    Håkansson, Joakim
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Ståhlberg, Anders
    University of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden.
    Patient-derived scaffolds uncover breast cancer promoting properties of the microenvironment2020Ingår i: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 235, artikel-id 119705Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Tumor cells interact with the microenvironment that specifically supports and promotes tumor development. Key components in the tumor environment have been linked to various aggressive cancer features and can further influence the presence of subpopulations of cancer cells with specific functions, including cancer stem cells and migratory cells. To model and further understand the influence of specific microenvironments we have developed an experimental platform using cell-free patient-derived scaffolds (PDSs) from primary breast cancers infiltrated with standardized breast cancer cell lines. This PDS culture system induced a series of orchestrated changes in differentiation, epithelial-mesenchymal transition, stemness and proliferation of the cancer cell population, where an increased cancer stem cell pool was confirmed using functional assays. Furthermore, global gene expression profiling showed that PDS cultures were similar to xenograft cultures. Mass spectrometry analyses of cell-free PDSs identified subgroups based on their protein composition that were linked to clinical properties, including tumor grade. Finally, we observed that an induction of epithelial-mesenchymal transition-related genes in cancer cells growing on the PDSs were significantly associated with clinical disease recurrences in breast cancer patients. Patient-derived scaffolds thus mimics in vivo-like growth conditions and uncovers unique information about the malignancy-inducing properties of tumor microenvironment. © 2019 The Authors

  • 15.
    Newman, Diane K
    et al.
    University of Pennsylvania, USA.
    New, Peter W
    Monash University, Australia; Caulfield Hospital, Australia.
    Heriseanu, Roxana
    Royal Rehab, Australia.
    Petronis, Sarunas
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Håkansson, Joakim
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Håkansson, Maria Å
    Wellspect, Sweden.
    Lee, Bonsan Bonne
    Prince of Wales Hospital, Australia.
    Intermittent catheterization with single- or multiple-reuse catheters: clinical study on safety and impact on quality of life2020Ingår i: International Urology and Nephrology, ISSN 0301-1623, E-ISSN 1573-2584, Vol. 19, nr 1, s. 1-153Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    PURPOSE: Intermittent catheterization (IC) is a proven effective long-term bladder management strategy for individuals who have lower urinary tract dysfunction. This study provides clinical evidence about multiple-reuse versus single-use catheterization techniques and if catheter choice can have an impact on health-related quality of life (HRQoL).

    METHOD: A prospective, multi-center, clinical trial studied patients who currently practiced catheter reuse, and who agreed to prospectively evaluate single-use hydrophilic-coated (HC) (i.e. LoFric) catheters for 4 weeks. A validated Intermittent Self-Catheterization Questionnaire (ISC-Q) was used to obtain HRQoL. Reused catheters were collected and studied with regard to microbial and debris contamination.

    RESULTS: The study included 39 patients who had practiced IC for a mean of 10 years, 6 times daily. At inclusion, all patients reused catheters for a mean of 21 days (SD = 48) per catheter. 36 patients completed the prospective test period and the mean ISC-Q score increased from 58.0 (SD = 22.6) to 67.2 (SD = 17.7) when patients switched to the single-use HC catheters (p = 0.0101). At the end of the study, 83% (95% CI [67-94%]) preferred to continue using single-use HC catheters. All collected reused catheters (100%) were contaminated by debris and 74% (95% CI [58-87%]) were contaminated by microorganisms, some with biofilm.

    CONCLUSION: Single-use HC catheters improved HRQoL and were preferred over catheter reuse among people practicing IC. Catheter multiple-reuse may pose a potential safety concern due to colonization by microorganisms as well as having reduced acceptance compared to single use.

    TRIAL REGISTRY NUMBER: ClinicalTrials.gov NCT02129738.

  • 16.
    Rosendahl, Jennifer
    et al.
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Svanström, Andreas
    University of Gothenburg, Sweden.
    Berglin, Mattias
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Petronis, Sarunas
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Bogestål, Yalda
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Stenlund, Patrik
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Standoft, Simon
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Ståhlberg, Anders
    University of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden.
    Landberg, Göran
    University of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden.
    Chinga-Carrasco, Gary
    RISE Research Institutes of Sweden, Bioekonomi och hälsa, Material- och ytdesign. University of Gothenburg, Sweden.
    Håkansson, Joakim
    University of Gothenburg, Sweden.
    3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System2021Ingår i: Bioengineering, E-ISSN 2306-5354, Vol. 8, nr 7, artikel-id 97Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Current conventional cancer drug screening models based on two-dimensional (2D) cell culture have several flaws and there is a large need of more in vivo mimicking preclinical drug screening platforms. The microenvironment is crucial for the cells to adapt relevant in vivo characteristics and here we introduce a new cell culture system based on three-dimensional (3D) printed scaffolds using cellulose nanofibrils (CNF) pre-treated with 2,2,6,6-tetramethylpyperidine-1-oxyl (TEMPO) as the structural material component. Breast cancer cell lines, MCF7 and MDA-MB-231, were cultured in 3D TEMPO-CNF scaffolds and were shown by scanning electron microscopy (SEM) and histochemistry to grow in multiple layers as a heterogenous cell population with different morphologies, contrasting 2D cultured mono-layered cells with a morphologically homogenous cell population. Gene expression analysis demonstrated that 3D TEMPO-CNF scaffolds induced elevation of the stemness marker CD44 and the migration markers VIM and SNAI1 in MCF7 cells relative to 2D control. T47D cells confirmed the increased level of the stemness marker CD44 and migration marker VIM which was further supported by increased capacity of holoclone formation for 3D cultured cells. Therefore, TEMPO-CNF was shown to represent a promising material for 3D cell culture model systems for cancer cell applications such as drug screening.

  • 17.
    Svanström, Andreas
    et al.
    University of Gothenburg, Sweden.
    Rosendahl, Jennifer
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Salerno, Simona
    University of Gothenburg, Sweden.
    Leiva, Maria
    University of Gothenburg, Sweden.
    Gregersson, Pernilla
    University of Gothenburg, Sweden.
    Berglin, Mattias
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Bogestål, Yalda
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Lausmaa, Jukka
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Oko, Asaf
    RISE Research Institutes of Sweden.
    Chinga-Carrasco, Gary
    RISE Research Institutes of Sweden, Bioekonomi och hälsa, Material- och ytdesign.
    Petronis, Sarunas
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Standoft, Simon
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Ståhlberg, Anders
    University of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden.
    Håkansson, Joakim
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil. University of Gothenburg, Sweden.
    Landberg, Göran
    University of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden.
    Optimized alginate-based 3D printed scaffolds as a model of patient derived breast cancer microenvironments in drug discovery2021Ingår i: Biomedical Materials, ISSN 1748-6041, E-ISSN 1748-605X, Vol. 16, nr 4, artikel-id 045046Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The cancer microenvironment influences tumor progression and metastasis and is pivotal to consider when designing in vivo-like cancer models. Current preclinical testing platforms for cancer drug development are mainly limited to 2D cell culture systems that poorly mimic physiological environments and traditional, low throughput animal models. The aim of this work was to produce a tunable testing platform based on 3D printed scaffolds (3DPS) with a simple geometry that, by extracellular components and response of breast cancer reporter cells, mimics patient-derived scaffolds (PDS) of breast cancer. Here, the biocompatible polysaccharide alginate was used as base material to generate scaffolds consisting of a 3D grid containing periostin and hydroxyapatite. Breast cancer cell lines (MCF7 and MDA-MB-231) produced similar phenotypes and gene expression levels of cancer stem cell, epithelial-mesenchymal transition, differentiation and proliferation markers when cultured on 3DPS and PDS, contrasting conventional 2D cultures. Importantly, cells cultured on 3DPS and PDS showed scaffold-specific responses to cytotoxic drugs (doxorubicin and 5-fluorouracil) that were different from 2D cultured cells. In conclusion, the data presented support the use of a tunable alginate-based 3DPS as a tumor model in breast cancer drug discovery. © 2021 The Author(s).

  • 18.
    Svensson, Sara
    et al.
    University of Gothenburg, Sweden.
    Trobos, Margarita
    University of Gothenburg, Sweden.
    Hoffman, Maria
    University of Gothenburg, Sweden.
    Norlindh, Birgitta
    University of Gothenburg, Sweden.
    Petronis, Sarunas
    RISE., SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Lausmaa, Jukka
    RISE., SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SP – Sveriges Tekniska Forskningsinstitut / Funktionella material (KMf).
    Suska, Felicia
    University of Gothenburg, Sweden.
    Thomsen, Peter
    University of Gothenburg, Sweden.
    A novel soft tissue model for biomaterial-associated infection and inflammation - Bacteriological, morphological and molecular observations2015Ingår i: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 41, s. 106-121Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Infection constitutes a major risk for implant failure, but the reasons why biomaterial sites are more vulnerable than normal tissue are not fully elucidated. In this study, a soft tissue infection model was developed, allowing the analysis of cellular and molecular responses in each of the sub-compartments of the implant-tissue interface (on the implant surface, in the surrounding exudate and in the tissue). Smooth and nanostructured titanium disks with or without noble metal chemistry (silver, gold, palladium), and sham sites, were inoculated with Staphylococcus epidermidis and analysed with respect to number of viable bacteria, number, viability and gene expression of host cells, and using different morphological techniques after 4 h, 24 h and 72 h. Non-infected rats were controls. Results showed a transient inflammatory response at control sites, whereas bacterial administration resulted in higher recruitment of inflammatory cells (mainly polymorphonuclear), higher, continuous cell death and higher gene expression of tumour necrosis factor-alpha, interleukin-6, interleukin-8, Toll-like receptor 2 and elastase. At all time points, S. epidermidis was predominantly located in the interface zone, extra- and intracellularly, and lower levels were detected on the implants compared with surrounding exudate. This model allows detailed analysis of early events in inflammation and infection associated to biomaterials in vivo leading to insights into host defence mechanisms in biomaterial-associated infections.

  • 19.
    Tasiopoulos, Christos
    et al.
    KTH Royal Institute of Technology, Sweden.
    Petronis, Sarunas
    RISE Research Institutes of Sweden, Material och produktion, Kemi, biomaterial och textil.
    Sahlin, Herman
    Neoss Ltd, Sweden.
    Hedhammar, My
    KTH Royal Institute of Technology, Sweden.
    Surface Functionalization of PTFE Membranes Intended for Guided Bone Regeneration Using Recombinant Spider Silk2020Ingår i: ACS Applied Bio Materials, E-ISSN 2576-6422, Vol. 3, nr 1, s. 577-583Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Alveolar bone loss is usually treated with guided bone regeneration, a dental procedure which utilizes a tissue-separation membrane. The barrier membrane prevents pathogens and epithelial cells to invade the bone augmentation site, thereby permitting osteoblasts to deposit minerals and build up bone. This study aims at adding bioactive properties to otherwise inert PTFE membranes in order to enhance cell adherence and promote proliferation. A prewetting by ethanol and stepwise hydration protocol was herein employed to overcome high surface tension of PTFE membranes and allow for a recombinant spider silk protein, functionalized with a cell-binding motif from fibronectin (FN-silk), to self-assemble into a nanofibrillar coating. HaCaT and U-2 OS cells were seeded onto soft and hard tissue sides, respectively, of membranes coated with FN-silk. The cells could firmly adhere as early as 1 h post seeding, as well as markedly grow in numbers when kept in culture for 7 days. Fluorescence and scanning electron microscopy images revealed that adherent cells could form a confluent monolayer and develop essential cell-cell contacts during 1 week of culture. Hence, functionalized PTFE membranes have a potential of better integration at the implantation site, with reduced risk of membrane displacement as well as exposure to oral pathogens.

  • 20.
    Wells, James
    et al.
    National Physical Laboratory, UK; Physikalisch-Technische Bundesanstalt, Germany.
    Kazakova, Olga
    National Physical Laboratory, UK.
    Posth, Oliver
    Physikalisch-Technische Bundesanstalt, Germany.
    Steinhoff, Uwe
    Physikalisch-Technische Bundesanstalt, Germany.
    Petronis, Sarunas
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Bogart, Lara
    Healthcare Biomagnetics Laboratory, UK.
    Southern, Paul
    Healthcare Biomagnetics Laboratory, UK.
    Pankhurst, Quentin
    Healthcare Biomagnetics Laboratory, UK.
    Johansson, Christer
    RISE - Research Institutes of Sweden, ICT, Acreo.
    Standardisation of magnetic nanoparticles in liquid suspension2017Ingår i: Journal of Physics D: Applied Physics, Vol. 50, nr 383003, s. 1-25Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Suspensions of magnetic nanoparticles offer diverse opportunities for technology innovation,spanning a large number of industry sectors from imaging and actuation based applicationsin biomedicine and biotechnology, through large-scale environmental remediation uses suchas water purification, to engineering-based applications such as position-controlled lubricantsand soaps. Continuous advances in their manufacture have produced an ever-growing rangeof products, each with their own unique properties. At the same time, the characterisation ofmagnetic nanoparticles is often complex, and expert knowledge is needed to correctly interpretthe measurement data. In many cases, the stringent requirements of the end-user technologiesdictate that magnetic nanoparticle products should be clearly defined, well characterised,consistent and safe; or to put it another way—standardised. The aims of this document areto outline the concepts and terminology necessary for discussion of magnetic nanoparticles,to examine the current state-of-the-art in characterisation methods necessary for the mostprominent applications of magnetic nanoparticle suspensions, to suggest a possible structurefor the future development of standardisation within the field, and to identify areas and topicswhich deserve to be the focus of future work items. We discuss potential roadmaps for thefuture standardisation of this developing industry, and the likely challenges to be encounteredalong the way.

  • 21.
    Wetterskog, E
    et al.
    Uppsala University, Sweden.
    Castro, A
    SOLVE Research and Consultancy AB, Sweden.
    Zeng, L
    Chalmers University of Technology, Sweden .
    Petronis, Sarunas
    RISE - Research Institutes of Sweden, Biovetenskap och material, Kemi och material.
    Heinke, D
    nanoPET Pharma GmbH, Germany.
    Olsson, E
    Chalmers University of Technology, Sweden .
    Nilsson, L
    Lund University, Sweden; SOLVE Research and Consultancy AB, Sweden.
    Gehrke, N
    nanoPET Pharma GmbH, Germany.
    Svedlindh, P
    Uppsala University, Sweden.
    Size and property bimodality in magnetic nanoparticle dispersions: single domain particles vs. strongly coupled nanoclusters2017Ingår i: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 9, nr 12, s. 4227-4235Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The widespread use of magnetic nanoparticles in the biotechnical sector puts new demands on fast and quantitative characterization techniques for nanoparticle dispersions. In this work, we report the use of asymmetric flow field-flow fractionation (AF4) and ferromagnetic resonance (FMR) to study the properties of a commercial magnetic nanoparticle dispersion. We demonstrate the effectiveness of both techniques when subjected to a dispersion with a bimodal size/magnetic property distribution: i.e., a small superparamagnetic fraction, and a larger blocked fraction of strongly coupled colloidal nanoclusters. We show that the oriented attachment of primary nanocrystals into colloidal nanoclusters drastically alters their static, dynamic, and magnetic resonance properties. Finally, we show how the FMR spectra are influenced by dynamical effects; agglomeration of the superparamagnetic fraction leads to reversible line-broadening; rotational alignment of the suspended nanoclusters results in shape-dependent resonance shifts. The AF4 and FMR measurements described herein are fast and simple, and therefore suitable for quality control procedures in commercial production of magnetic nanoparticles.

  • 22.
    Österberg, Klas
    et al.
    University of Gothenburg, Sweden.
    Bogestål, Yalda
    RISE Research Institutes of Sweden, Material och produktion, Metodik för produktframtagning.
    Jenndahl, Lachmi
    VERIGRAFT AB, Sweden.
    Gustafsson-Hedberg, Tobias
    VERIGRAFT AB, Sweden.
    Synnergren, Jane
    University of Gothenburg, Sweden; University of Skövde, Sweden.
    Holmgren, Gustav
    University of Skövde, Sweden.
    Bom, Eva
    RISE Research Institutes of Sweden, Material och produktion, Metodik för produktframtagning.
    Petronis, Sarunas
    RISE Research Institutes of Sweden, Material och produktion, Metodik för produktframtagning.
    Krona, Annika
    RISE Research Institutes of Sweden, Bioekonomi och hälsa, Jordbruk och livsmedel.
    Eriksson, Jonna
    TATAA Biocenter AB, Sweden.
    Rosendahl, Jennifer
    RISE Research Institutes of Sweden, Material och produktion, Metodik för produktframtagning.
    Crisostomo, Veronica
    Jesús Usón Minimally Invasive Surgery Centre, Spain; CIBER de Enfermedades Cardiovasculares, Spain; RICORS-TERAV Network, Spain.
    Sanchez-Margallo, Francisco
    Jesús Usón Minimally Invasive Surgery Centre, Spain; CIBER de Enfermedades Cardiovasculares, Spain; RICORS-TERAV Network, Spain.
    Baez-Diaz, Claudia
    Jesús Usón Minimally Invasive Surgery Centre, Spain; CIBER de Enfermedades Cardiovasculares, Spain; RICORS-TERAV Network, Spain.
    Strehl, Raimund
    VERIGRAFT AB, Sweden.
    Håkansson, Joakim
    RISE Research Institutes of Sweden, Material och produktion, Metodik för produktframtagning. University of Gothenburg, Sweden.
    Personalized tissue-engineered veins - long term safety, functionality and cellular transcriptome analysis in large animals2023Ingår i: Biomaterials Science, ISSN 2047-4830, E-ISSN 2047-4849, Vol. 11, nr 11, s. 3860-3877Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Tissue engineering is a promising methodology to produce advanced therapy medicinal products (ATMPs). We have developed personalized tissue engineered veins (P-TEV) as an alternative to autologous or synthetic vascular grafts utilized in reconstructive vein surgery. Our hypothesis is that individualization through reconditioning of a decellularized allogenic graft with autologous blood will prime the tissue for efficient recellularization, protect the graft from thrombosis, and decrease the risk of rejection. In this study, P-TEVs were transplanted to vena cava in pig, and the analysis of three veins after six months, six veins after 12 months and one vein after 14 months showed that all P-TEVs were fully patent, and the tissue was well recellularized and revascularized. To confirm that the ATMP product had the expected characteristics one year after transplantation, gene expression profiling of cells from P-TEV and native vena cava were analyzed and compared by qPCR and sequencing. The qPCR and bioinformatics analysis confirmed that the cells from the P-TEV were highly similar to the native cells, and we therefore conclude that P-TEV is functional and safe in large animals and have high potential for use as a clinical transplant graft.

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