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  • 1.
    Assenhöj, Maria
    et al.
    Linköping University, Sweden.
    Eriksson, Peter
    Linköping University, Sweden.
    Dönnes, Pierre
    SciCross AB, Sweden.
    Ljunggren, Stefan
    Linköping University, Sweden.
    Marcusson-Ståhl, Maritha
    RISE Research Institutes of Sweden, Bioeconomy and Health, Chemical Process and Pharmaceutical Development.
    Du Rietz, Anna
    Linköping University, Sweden.
    Uvdal, Kajsa
    Linköping University, Sweden.
    Karlsson, Hanna
    Linköping University, Sweden.
    Cederbrant, Karin
    RISE Research Institutes of Sweden.
    Protein interaction, monocyte toxicity and immunogenic properties of cerium oxide crystals with 5% or 14% gadolinium, cobalt oxide and iron oxide nanoparticles–an interdisciplinary approach2021In: Nanotoxicology, ISSN 1743-5390, E-ISSN 1743-5404, Vol. 15, no 8, p. 1035-1038Article in journal (Refereed)
    Abstract [en]

    Metal oxide nanoparticles are widely used in both consumer products and medical applications, but the knowledge regarding exposure-related health effects is limited. However, it is challenging to investigate nanoparticle interaction processes with biological systems. The overall aim of this project was to improve the possibility to predict exposure-related health effects of metal oxide nanoparticles through interdisciplinary collaboration by combining workflows from the pharmaceutical industry, nanomaterial sciences, and occupational medicine. Specific aims were to investigate nanoparticle-protein interactions and possible adverse immune reactions. Four different metal oxide nanoparticles; CeOx nanocrystals with 5% or 14% Gd, Co3O4, and Fe2O3, were characterized by dynamic light scattering and high-resolution transmission electron microscopy. Nanoparticle-binding proteins were identified and screened for HLA-binding peptides in silico. Monocyte interaction with nanoparticle–protein complexes was assessed in vitro. Herein, for the first time, immunogenic properties of nanoparticle-binding proteins have been characterized. The present study indicates that especially Co3O4-protein complexes can induce both ‘danger signals’, verified by the production of inflammatory cytokines and simultaneously bind autologous proteins, which can be presented as immunogenic epitopes by MHC class II. The clinical relevance of these findings should be further evaluated to investigate the role of metal oxide nanoparticles in the development of autoimmune disease. The general workflow identified experimental difficulties, such as nanoparticle aggregate formation and a lack of protein-free buffers suitable for particle characterization, protein analyses, as well as for cell studies. This confirms the importance of future interdisciplinary collaborations. © 2021 The Author(s). 

  • 2.
    Eriksson, Peter
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Carlsson, Bo
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Accelerated corrosion test involving alternate exposure for hostile gases, neutral salt spray and drying. SP method 24991998Report (Refereed)
    Download full text (pdf)
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  • 3.
    Mölmen, Live
    et al.
    RISE Research Institutes of Sweden, Safety and Transport, Electrification and Reliability. Jönköping University, Sweden.
    Fast, Lars
    RISE Research Institutes of Sweden, Safety and Transport, Electrification and Reliability.
    Lundblad, Anders Olof
    RISE Research Institutes of Sweden, Safety and Transport, Electrification and Reliability.
    Eriksson, Peter
    RISE Research Institutes of Sweden, Materials and Production, Corrosion.
    Leisner, Peter
    Jönköping University, Sweden.
    Contact resistance measurement methods for PEM fuel cell bipolar plates and power terminals2023In: Journal of Power Sources, ISSN 0378-7753, E-ISSN 1873-2755, Vol. 555, article id 232341Article in journal (Refereed)
    Abstract [en]

    The electrical contact resistance is a key parameter for optimising both the bipolar plate of the polymer electrolyte membrane fuel cell (PEMFC) and the electrical contact of the power terminal of the stack. The contact resistance is affected by the conductivity, roughness, and hardness of the two contacting surfaces. Here, new, application-specific contact resistance measurement methods are proposed for both the stack power terminal, and the bipolar plate. The proposed methods are compared to methods from references as well as standards, and it is concluded that the uncertainty of the measurements can be reduced by changing the measurement setup, and that the influence of probe resistance on measurement results can be eliminated. Furthermore, the effect of different accelerated durability tests on the contact resistance of the power terminal is examined both on test coupons and on a prototype screw connection with an electroless NiP and an electroplated NiSn coatings. As expected, the NiSn coupons gives lower contact resistance after ageing as compared to the NiP. However, the increase in contact resistance seen on coupons after ageing is not observed on the prototype screw connection. © 2022 The Author(s)

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