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  • 1. Andersson, J
    et al.
    Stenhamre, H
    Bäckdahl, Henrik
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Gatenholm, P
    Behaviour of human chondrocytes in engineered porous bacterial cellulose scaffolds. J Biomed Mater Res: Part A. 94A2010In: J Biomed Mater Res: Part A., Vol. 94A, no 4, p. 1124-1132Article in journal (Refereed)
  • 2.
    Björn, Camilla
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. University of Gothenburg, Sahlgrenska Academy, Institute of Medicine, Department of Molecular and Clinical Medicine.
    Antimicrobial peptides in the treatment of infectious and inflammatory conditions: Preclinical studies of mechanism of action, efficacy, and safety2016Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    The rapid emergence of antibiotic-resistant microbes worldwide and the urgent need of new antimicrobial agents have stimulated interest in antimicrobial peptides (AMPs) as new therapeutics for treatment of infectious diseases. AMPs are present in all living species and constitute an important part of the innate immune system in multicellular organisms, including humans. AMPs display a remarkably broad spectrum of antimicrobial activity covering both Gram-positive and Gram-negative bacteria, including many antibiotic-resistant strains, as well as fungi, viruses, and protozoa. Further, in contrast to many conventional antibiotics, AMPs rapidly kill bacteria instead of just inhibiting bacterial growth. In addition, AMPs act as modulators of the innate immune system and, importantly, bacteria seem less efficient in developing resistance towards AMPs than towards conventional antibiotics. Together these properties make AMPs highly attractive as a new class of antimicrobials, with clinical potential also extending to diseases where inflammation is part of the pathology. The aim of this thesis was to study novel AMPs with respect to their mechanism of action (MOA), antimicrobial spectrum, propensity to select for resistance, and in vivo efficacy and safety. To achieve this, we used a number of in vitro and in vivo assays, together generating a comprehensive preclinical evaluation of the peptides. The hypothesis was that the AMPs in this thesis have potential to be developed as therapeutic agents for several infectious and inflammatory conditions, including treatment of skin and soft tissue infections and prevention of postsurgical adhesion formation. The results showed that all AMPs tested (i.e. PXL03, PXL150, HLR1r, and five variants of CEN1 HC-Br) had broad antimicrobial spectra in vitro with varying sensitivity to salt and serum. Furthermore, PXL150 caused a rapid permeabilization of bacterial membrane in vitro, indicating that this is at least one part of the MOA of this peptide. Under selection pressure in vitro, bacteria did not develop resistance to the peptides tested, i.e. PXL150 and CEN1 HC. Interestingly, all peptides showed anti-inflammatory activity by inhibiting the secretion of proinflammatory mediators from stimulated human cell lines. In addition, PXL01, PXL150, and HLR1r demonstrated fibrinolytic ability in vitro by suppressing the release of plasminogen activator inhibitor-1 (PAI-1). In ex vivo and in vivo skin/wound infection models, the peptides reduced the number of viable bacteria and yeast cells. Further, PXL01 decreased postsurgical adhesion formation in vivo. Notably, nonclinical safety studies showed that PXL150 was safe and well tolerated. In conclusion, several of the peptides evaluated in this thesis demonstrated a promising preclinical efficacy and safety profile motivating further development as drug candidates for local treatment of infectious and inflammatory conditions.

  • 3.
    Björn, Camilla
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Mahlapuu, M.
    Mattsby-Baltzer, I.
    Håkansson, Joakim
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Anti-infective efficacy of the lactoferrin-derived antimicrobial peptide HLR1r2016In: Peptides, ISSN 0196-9781, E-ISSN 1873-5169, Vol. 81, p. 21-28Article in journal (Refereed)
    Abstract [en]

    Antimicrobial peptides (AMPs) have emerged as a new class of drug candidates for the treatment of infectious diseases. Here we describe a novel AMP, HLR1r, which is structurally derived from the human milk protein lactoferrin and demonstrates a broad spectrum microbicidal action in vitro. The minimum concentration of HLR1r needed for killing ≥99% of microorganisms in vitro, was in the range of 3-50 μg/ml for common Gram-negative and Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), and for the yeast Candida albicans, when assessed in diluted brain-heart infusion medium. We found that HLR1r also possesses anti-inflammatory properties as evidenced by inhibition of tumor necrosis factor alpha (TNF-α) secretion from human monocyte-derived macrophages and by repression of interleukin-6 (IL-6) and plasminogen activator inhibitor-1 (PAI-1) secretion from human mesothelial cells, without any cytotoxic effect observed at the concentration range tested (up to 400 μg/ml). HLR1r demonstrated pronounced anti-infectious effect in in vivo experimental models of cutaneous candidiasis in mice and of excision wounds infected with MRSA in rats as well as in an ex vivo model of pig skin infected with S. aureus. In conclusion, HLR1r may constitute a new therapeutic alternative for local treatment of skin infections.

  • 4.
    Bogestål, Yalda
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Identification of novel biomarkers for doxorubicin-induced toxicity in human cardiomyocytes derived from pluripotent stem cells2015In: Toxicology, ISSN 0300-483X, E-ISSN 1879-3185, Vol. 328, no Febr, p. 102-111Article in journal (Refereed)
  • 5.
    Brive, Lena
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SP – Sveriges Tekniska Forskningsinstitut / Organisk kemi (Kmo).
    Pinori, Emiliano
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Multi-seasonal barnacle (Balanus improvisus) protection achieved by trace amounts of a macrocyclic lactone (ivermectin) included in rosin-based coatings2011In: Biofouling (Print), ISSN 0892-7014, E-ISSN 1029-2454, Vol. 27, no 9, p. 941-953Article in journal (Refereed)
  • 6.
    Bäckdahl, Henrik
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    In vivo application of tissue-engineered veins using autologous peripheral whole blood: A proof of concept study2014In: EBioMedicine, ISSN 0360-0637, E-ISSN 2352-3964, Vol. 1, no 1, p. 72-79Article in journal (Refereed)
  • 7.
    Bäckdahl, Henrik
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Successful tissue engineering of competent allogeneic venous valves2015In: Journal of Vascular Surgery: Venous and Lymphatic Disorders, ISSN 2213-333X, Vol. 3, no 4, p. 421-30Article in journal (Refereed)
  • 8.
    Bäckdahl, Henrik
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Towards the development of a bioengineered uterus: Comparison of different protocols for rat uterus decellularization2014In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 10, no 12, p. 5034-5042Article in journal (Refereed)
  • 9.
    Bäckdahl, Henrik
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Risberg, B
    Gatenholm, P
    Observations on bacterial cellulose tube formation for application as vascular graft.2011In: Materials Science and Engineering, Vol. 31, p. 14-21Article in journal (Refereed)
  • 10.
    Carlred, Louise
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. Department of Physics, Chalmers University of Technology.
    Michno, W.
    Kaya, I.
    Sjövall, Peter
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. Department of Physics, Chalmers University of Technology.
    Syvänen, S.
    Hanrieder, J.
    Probing amyloid-β pathology in transgenic Alzheimer's disease (tgArcSwe) mice using MALDI imaging mass spectrometry2016In: Journal of Neurochemistry, ISSN 0022-3042, E-ISSN 1471-4159, Vol. 138, no 3, p. 469-478Article in journal (Refereed)
    Abstract [en]

    The pathological mechanisms underlying Alzheimer's disease (AD) are still not understood. The disease pathology is characterized by the accumulation and aggregation of amyloid-β (Aβ) peptides into extracellular plaques, however the factors that promote neurotoxic Aβ aggregation remain elusive. Imaging mass spectrometry (IMS) is a powerful technique to comprehensively elucidate the spatial distribution patterns of lipids, peptides and proteins in biological tissues. In the present study, matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS)-based imaging was used to study Aβ deposition in transgenic mouse brain tissue and to elucidate the plaque-associated chemical microenvironment. The imaging experiments were performed in brain sections of transgenic Alzheimer's disease mice carrying the Arctic and Swedish mutation of amyloid-beta precursor protein (tgArcSwe). Multivariate image analysis was used to interrogate the IMS data for identifying pathologically relevant, anatomical features based on their chemical identity. This include cortical and hippocampal Aβ deposits, whose amyloid peptide content was further verified using immunohistochemistry and laser microdissection followed by MALDI MS analysis. Subsequent statistical analysis on spectral data of regions of interest revealed brain region-specific differences in Aβ peptide aggregation. Moreover, other plaque-associated protein species were identified including macrophage migration inhibitory factor suggesting neuroinflammatory processes and glial cell reactivity to be involved in AD pathology. The presented data further highlight the potential of IMS as a powerful approach in neuropathology. Hanrieder et al. described an imaging mass spectrometry based study on comprehensive spatial profiling of C-terminally truncated Aβ species within individual plaques in tgArcSwe mice. Here, brain region-dependent differences in Aβ truncation and other plaque-associated proteins, such as macrophage migration inhibitory factor, were observed. The data shed further light on plaque-associated molecular mechanisms implicated in Alzheimer's pathogenesis.

  • 11.
    Carlred, Louise
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Sjövall, Peter
    Funktionella material (KMf).
    Simultaneous imaging of amyloid-β and lipids in brain tissue using antibody-coupled liposomes and time-of-flight secondary ion mass spectrometry2014In: Journal of the American Chemical Society, ISSN 0002-7863, E-ISSN 1520-5126, Vol. 136, no 28, p. 9973-9981Article in journal (Refereed)
  • 12.
    Carlred, Louise
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. Department of Physics, Chalmers University of Technology.
    Vukojević, V.
    Johansson, B.
    Schalling, M.
    Höök, F.
    Sjövall, Peter
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. Department of Applied Physics, Chalmers University of Technology.
    Imaging of amyloid-β in alzheimer’s disease transgenic mouse brains with ToF-SIMS using immunoliposomes2016In: Biointerphases, ISSN 1934-8630, E-ISSN 1559-4106, Vol. 11, no 2, p. 1-11, article id 02A312Article in journal (Refereed)
    Abstract [en]

    Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has been proven to successfully image different kinds of molecules, especially a variety of lipids, in biological samples. Proteins, however, are difficult to detect as specific entities with this method due to extensive fragmentation. To circumvent this issue, the authors present in this work a method developed for detection of proteins using antibody-conjugated liposomes, so called immunoliposomes, which are able to bind to the specific protein of interest. In combination with the capability of ToF-SIMS to detect native lipids in tissue samples, this method opens up the opportunity to analyze many different biomolecules, both lipids and proteins, at the same time, with high spatial resolution. The method has been applied to detect and image the distribution of amyloid-β (Aβ), a biologically relevant peptide in Alzheimer’s disease (AD), in transgenic mouse brain tissue. To ensure specific binding, the immunoliposome binding was verified on a model surface using quartz crystal microbalance with dissipation monitoring. The immunoliposome binding was also investigated on tissue sections with fluorescence microscopy, and compared with conventional immunohistochemistry using primary and secondary antibodies, demonstrating specific binding to Aβ. Using ToF-SIMS imaging, several endogenous lipids, such as cholesterol and sulfatides, were also detected in parallel with the immunoliposome-labeled Aβ deposits, which is an advantage compared to fluorescence microscopy. This method can thus potentially provide further information about lipid–protein interactions, which is important to understand the mechanisms of neurodegeneration in AD.

  • 13. Colombo, S.
    et al.
    Brisander, M.
    Haglöf, J.
    Sjövall, Peter
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Andersson, P.
    Østergaard, J.
    Malmsten, M.
    Matrix effects in nilotinib formulations with pH-responsive polymer produced by carbon dioxide-mediated precipitation2015In: International Journal of Pharmaceutics, ISSN 0378-5173, E-ISSN 1873-3476, Vol. 494, no 1, p. 205-217, article id 15114Article in journal (Refereed)
    Abstract [en]

    Factors determining the pH-controlled dissolution kinetics of nilotinib formulations with the pH-titrable polymer hydroxypropyl methylcellulose phthalate, obtained by carbon dioxide-mediated precipitation, were mechanistically examined in acid and neutral environment. The matrix effect, modulating the drug dissolution, was characterized with a battery of physicochemical methodologies, including ToF-SIMS for surface composition, SAXS/WAXS and modulated DSC for crystallization characterization, and simultaneous UV-imaging and Raman spectroscopy for monitoring the dissolution process in detail. The hybrid particle formulations investigated consisted of amorphous nilotinib embedded in a polymer matrix in single continuous phase, displaying extended retained amorphicity also under wet conditions. It was demonstrated by Raman and FTIR spectroscopy that the efficient drug dispersion and amorphization in the polymer matrix were mediated by hydrogen bonding between the drug and the phthalate groups on the polymer. Simultaneous Raman and UV-imaging studies of the effect of drug load on the swelling and dissolution of the polymer matrix revealed that high nilotinib load prevented matrix swelling on passage from acid to neutral pH, thereby preventing re-precipitation and re-crystallization of incorporated nilotinib. These findings provide a mechanistic foundation of formulation development of nilotinib and other protein kinase inhibitors, which are now witnessing an intense therapeutic and industrial attention due to the difficulty in formulating these compounds so that efficient oral bioavailability is reached.

  • 14.
    Dahlström, Mia
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Berglin, Mattias
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Pinori, Emiliano
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Affinity states of biocides determine bioavailability and release rates in marine paints2015In: Biofouling (Print), ISSN 0892-7014, E-ISSN 1029-2454, Vol. 31, no 2, p. 201-210Article in journal (Refereed)
  • 15.
    Fant, Kristina
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Enhanced cellular uptake of antisecretory peptide AF-16 through proteoglycan binding2014In: Biochemistry, ISSN 0006-2960, E-ISSN 1520-4995, Vol. 53, no 41, p. 6566-6573Article in journal (Refereed)
  • 16.
    Fant, Kristina
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Functionalization with C-terminal cysteine enhances transfection efficiency of cell-penetrating peptides through dimer formation2012In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 418, no 3, p. 469-474Article in journal (Refereed)
  • 17. Fray, Nicolas
    et al.
    Bardyn, Anaïs
    Cottin, Hervé
    Altwegg, Kathrin
    Baklouti, Donia
    Briois, Christelle
    Colangeli, Luigi
    Engrand, Cécile
    Fischer, Henning
    Glasmachers, Albrecht
    Grün, Eberhard
    Haerendel, Gerhard
    Henkel, Hartmut
    Höfner, Herwig
    Hornung, Klaus
    Jessberger, Elmar K.
    Koch, Andreas
    Krüger, Harald
    Langevin, Yves
    Lehto, Harry
    Lehto, Kirsi
    Le Roy, Léna
    Merouane, Sihane
    Modica, Paola
    Orthous-Daunay, François-Régis
    Paquette, John
    Raulin, François
    Rynö, Jouni
    Schulz, Rita
    Silén, Johan
    Siljeström, Sandra
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Steiger, Wolfgang
    Stenzel, Oliver
    Stephan, Thomas
    Thirkell, Laurent
    Thomas, Roger
    Torkar, Klaus
    Varmuza, Kurt
    Wanczek, Karl-Peter
    Zaprudin, Boris
    Kissel, Jochen
    Hilchenbach, Martin
    High-molecular-weight organic matter in the particles of comet 67P/Churyumov–Gerasimenko2016In: Nature, ISSN 0028-0836, E-ISSN 1476-4687, Vol. 538, no 7623, p. 72-74Article in journal (Refereed)
    Abstract [en]

    The presence of solid carbonaceous matter in cometary dust was established by the detection of elements such as carbon, hydrogen, oxygen and nitrogen in particles from comet 1P/Halley1, 2. Such matter is generally thought to have originated in the interstellar medium3, but it might have formed in the solar nebula—the cloud of gas and dust that was left over after the Sun formed4. This solid carbonaceous material cannot be observed from Earth, so it has eluded unambiguous characterization5. Many gaseous organic molecules, however, have been observed6, 7, 8, 9; they come mostly from the sublimation of ices at the surface or in the subsurface of cometary nuclei8. These ices could have been formed from material inherited from the interstellar medium that suffered little processing in the solar nebula10. Here we report the in situ detection of solid organic matter in the dust particles emitted by comet 67P/Churyumov–Gerasimenko; the carbon in this organic material is bound in very large macromolecular compounds, analogous to the insoluble organic matter found in the carbonaceous chondrite meteorites11, 12. The organic matter in meteorites might have formed in the interstellar medium and/or the solar nebula, but was almost certainly modified in the meteorites’ parent bodies11. We conclude that the observed cometary carbonaceous solid matter could have the same origin as the meteoritic insoluble organic matter, but suffered less modification before and/or after being incorporated into the comet.

  • 18.
    Fureby, Anna
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
    Dahlenborg, Hanna
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Effect of particle size in chocolate shell on oil migration and fat bloom development2015In: Journal of Food Engineering, ISSN 0260-8774, E-ISSN 1873-5770, Vol. 146, no Febr, p. 172-181Article in journal (Refereed)
  • 19. Goetz, W.
    et al.
    Brinckerhoff, W. B.
    Arevalo, R.
    Freissinet, C.
    Getty, S.
    Glavin, D. P.
    Siljeström, Sandra
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Buch, A.
    Stalport, F.
    Grubisic, A.
    Li, X.
    Pinnick, V.
    Danell, R.
    Van Amerom, F. H. W.
    Goesmann, F.
    Steininger, H.
    Grand, N.
    Raulin, F.
    Szopa, C.
    Meierhenrich, U.
    Brucato, J. R.
    MOMA: The challenge to search for organics and biosignatures on Mars2016In: International Journal of Astrobiology, ISSN 1473-5504, E-ISSN 1475-3006, Vol. 15, no 3, p. 239-250Article in journal (Refereed)
    Abstract [en]

    This paper describes strategies to search for, detect, and identify organic material on the surface and subsurface of Mars. The strategies described include those applied by landed missions in the past and those that will be applied in the future. The value and role of ESA's ExoMars rover and of her key science instrument Mars Organic Molecule Analyzer (MOMA) are critically assessed.

  • 20.
    Gunnarsson, Anders
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Bally, M
    Svensson, L
    Larsson, G
    Zhdanov, V P
    Hook, F
    Interaction of single viruslike particles with vesicles containing glycosphingolipids2011In: Physical Review Letters, Vol. 107, p. 188103-Article in journal (Refereed)
  • 21.
    Gunnarsson, Anders
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Dexlin, L
    Wallin, P
    Svedhem, S
    Jönsson, P
    Wingren, C
    Höök, F
    Kinetics of Ligand Binding to Membrane Receptors from Equilibrium Fluctuation Analysis of Single Binding Events,2011In: Journal of the American Chemical Society, Vol. 133, p. 14852–14855-Article in journal (Refereed)
  • 22.
    Gunnarsson, Anders
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Kollmer, Felix
    Sohn, Sascha
    Höök, Fredrik
    Sjövall, Peter
    Funktionella material (KMf).
    High-resolution mass spectrometry imaging of supported lipid bilayers and individual lipid vesicles2010In: Analytical Chemistry, Vol. 82, p. 2426-2433Article in journal (Refereed)
  • 23.
    Gunnarsson, Anders
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Sjövall, Peter
    Funktionella material (KMf).
    Höök, Fredrik
    Liposome-based bio-barcodes for on-chip DNA detection using imaging mass spectrometry2010In: Nano Letters, Vol. 10, p. 732-737Article in journal (Refereed)
  • 24. Hilchenbach, M.
    et al.
    Kissel, J.
    Langevin, Y.
    Briois, C.
    Hoerner, H. V.
    Koch, A.
    Schulz, R.
    Silén, J.
    Altwegg, K.
    Colangeli, L.
    Cottin, H.
    Engrand, C.
    Fischer, H.
    Glasmachers, A.
    Grün, E.
    Haerendel, G.
    Henkel, H.
    Höfner, H.
    Hornung, K.
    Jessberger, E. K.
    Lehto, H.
    Lehto, K.
    Raulin, F.
    Roy, L. L.
    Rynö, J.
    Steiger, W.
    Stephan, T.
    Thirkell, L.
    Thomas, R.
    Torkar, K.
    Varmuza, K.
    Wanczek, K. -P
    Altobelli, N.
    Baklouti, D.
    Bardyn, A.
    Fray, N.
    Krüger, H.
    Ligier, N.
    Lin, Z.
    Martin, P.
    Merouane, S.
    Orthous-Daunay, F. R.
    Paquette, J.
    Revillet, C.
    Siljeström, Sandra
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Stenzel, O.
    Zaprudin, B.
    COMET 67P/CHURYUMOV-GERASIMENKO: CLOSE-UP on DUST PARTICLE FRAGMENTS2016In: Astrophysical Journal Letters, ISSN 2041-8205, E-ISSN 2041-8213, Vol. 816, no 2, article id L32Article in journal (Refereed)
    Abstract [en]

    The COmetary Secondary Ion Mass Analyser instrument on board ESA's Rosetta mission has collected dust particles in the coma of comet 67P/Churyumov-Gerasimenko. During the early-orbit phase of the Rosetta mission, particles and particle agglomerates have been imaged and analyzed in the inner coma at distances between 100 km and 10 km off the cometary nucleus and at more than 3 AU from the Sun. We identified 585 particles of more than 14 μm in size. The particles are collected at low impact speeds and constitute a sample of the dust particles in the inner coma impacting and fragmenting on the targets. The sizes of the particles range from 14 μm up to sub-millimeter sizes and the differential dust flux size distribution is fitted with a power law exponent of -3.1. After impact, the larger particles tend to stick together, spread out or consist of single or a group of clumps, and the flocculent morphology of the fragmented particles is revealed. The elemental composition of the dust particles is heterogeneous and the particles could contain typical silicates like olivine and pyroxenes, as well as iron sulfides. The sodium to iron elemental ratio is enriched with regard to abundances in CI carbonaceous chondrites by a factor from ∼1.5 to ∼15. No clear evidence for organic matter has been identified. The composition and morphology of the collected dust particles appear to be similar to that of interplanetary dust particles.

  • 25.
    Håkansson, Joakim
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Björn, Camilla
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Efficacy and safety profile of the novel antimicrobial peptide PXL150 in a mouse model of infected burn wounds2015In: International Journal of Antimicrobial Agents, ISSN 0924-8579, E-ISSN 1872-7913, Vol. 45, no 5, p. 519-24Article in journal (Refereed)
  • 26.
    Håkansson, Joakim
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Björn, Camilla
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Efficacy of the novel topical antimicrobial agent pxl150 in a mouse model of surgical site infections2014In: Antimicrobial Agents and Chemotherapy, ISSN 0066-4804, E-ISSN 1098-6596, Vol. 58, no 5, p. 2982-2984Article in journal (Refereed)
  • 27. Karazisis, D.
    et al.
    Ballo, A. M.
    Petronis, Sarunas
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Agheli, H.
    Emanuelsson, L.
    Thomsen, P.
    Omar, O.
    The role of well-defined nanotopography of titanium implants on osseointegration: Cellular and molecular events in vivo2016In: International Journal of Nanomedicine, ISSN 1176-9114, E-ISSN 1178-2013, Vol. 11, p. 1367-1382Article in journal (Refereed)
    Abstract [en]

    Purpose: Mechanisms governing the cellular interactions with well-defined nanotopography are not well described in vivo. This is partly due to the difficulty in isolating a particular effect of nanotopography from other surface properties. This study employed colloidal lithography for nanofabrication on titanium implants in combination with an in vivo sampling procedure and different analytical techniques. The aim was to elucidate the effect of well-defined nanotopography on the molecular, cellular, and structural events of osseointegration. Materials and methods: Titanium implants were nanopatterned (Nano) with semispherical protrusions using colloidal lithography. Implants, with and without nanotopography, were implanted in rat tibia and retrieved after 3, 6, and 28 days. Retrieved implants were evaluated using quantitative polymerase chain reaction, histology, immunohistochemistry, and energy dispersive X-ray spectroscopy (EDS). Results: Surface characterization showed that the nanotopography was well defined in terms of shape (semispherical), size (79±6 nm), and distribution (31±2 particles/μm2). EDS showed similar levels of titanium, oxygen, and carbon for test and control implants, confirming similar chemistry. The molecular analysis of the retrieved implants revealed that the expression levels of the inflammatory cytokine, TNF-α, and the osteoclastic marker, CatK, were reduced in cells adherent to the Nano implants. This was consistent with the observation of less CD163-positive macrophages in the tissue surrounding the Nano implant. Furthermore, periostin immunostaining was frequently detected around the Nano implant, indicating higher osteogenic activity. This was supported by the EDS analysis of the retrieved implants showing higher content of calcium and phosphate on the Nano implants. Conclusion: The results show that Nano implants elicit less periimplant macrophage infiltration and downregulate the early expression of inflammatory (TNF-α) and osteoclastic (CatK) genes. Immunostaining and elemental analyses show higher osteogenic activity at the Nano implant. It is concluded that an implant with the present range of well-defined nanocues attenuates the inflammatory response while enhancing mineralization during osseointegration.

  • 28.
    Karlsson, Joakim
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Lausmaa, Jukka
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SP – Sveriges Tekniska Forskningsinstitut / Funktionella material (KMf).
    Characterization and comparison of materials produced by Electron Beam Melting (EBM) of two different Ti-6Al-4V powder fractions2013In: Journal of Materials Processing Technology, ISSN 0924-0136, E-ISSN 1873-4774, Vol. 213, no 12, p. 2109-2118Article in journal (Refereed)
  • 29.
    Karlsson, Joakim
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Lausmaa, Jukka
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SP – Sveriges Tekniska Forskningsinstitut / Funktionella material (KMf).
    Surface oxidation behavior of Ti-6Al-4V manufactured by Electron Beam Melting (EBM®)2015In: Journal of Manufacturing Processes, ISSN 1526-6125, Vol. 17, no Jan, p. 120-126Article in journal (Refereed)
  • 30. Krüger, H.
    et al.
    Stephan, T.
    Engrand, C.
    Briois, C.
    Siljeström, Sandra
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Merouane, S.
    Baklouti, D.
    Fischer, H.
    Fray, N.
    Hornung, K.
    Lehto, H.
    Orthous-Daunay, F. -R
    Rynö, J.
    Schulz, R.
    Silén, J.
    Thirkell, L.
    Trieloff, M.
    Hilchenbach, M.
    COSIMA-Rosetta calibration for in situ characterization of 67P/Churyumov-Gerasimenko cometary inorganic compounds2015In: Planetary and Space Science, ISSN 0032-0633, E-ISSN 1873-5088, Vol. 117, p. 35-44Article in journal (Refereed)
    Abstract [en]

    COmetary Secondary Ion Mass Analyzer (COSIMA) is a time-of-flight secondary ion mass spectrometry (TOF-SIMS) instrument on board the Rosetta space mission. COSIMA has been designed to measure the composition of cometary dust particles. It has a mass resolution m/Δm of 1400 at mass 100 u, thus enabling the discrimination of inorganic mass peaks from organic ones in the mass spectra. We have evaluated the identification capabilities of the reference model of COSIMA for inorganic compounds using a suite of terrestrial minerals that are relevant for cometary science. Ground calibration demonstrated that the performances of the flight model were similar to that of the reference model. The list of minerals used in this study was chosen based on the mineralogy of meteorites, interplanetary dust particles and Stardust samples. It contains anhydrous and hydrous ferromagnesian silicates, refractory silicates and oxides (present in meteoritic Ca-Al-rich inclusions), carbonates, and Fe-Ni sulfides. From the analyses of these minerals, we have calculated relative sensitivity factors for a suite of major and minor elements in order to provide a basis for element quantification for the possible identification of major mineral classes present in the cometary particles.

  • 31. Labandeira, C. C.
    et al.
    Yang, Q.
    Santiago-Blay, J. A.
    Hotton, C. L.
    Monteiro, A.
    Wang, Y. -J
    Goreva, Y.
    Shih, C. K.
    Siljeström, Sandra
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Rose, T. R.
    Dilcher, D. L.
    Ren, D.
    The evolutionary convergence of mid-mesozoic lacewings and cenozoic butterflies2016In: Proceedings of the Royal Society of London. Biological Sciences, ISSN 0962-8452, E-ISSN 1471-2954, Vol. 283, no 1824, article id 20152893Article in journal (Refereed)
    Abstract [en]

    Mid-Mesozoic kalligrammatid lacewings (Neuroptera) entered the fossil record 165 million years ago (Ma) and disappeared 45 Ma later. Extant papilionoid butterflies (Lepidoptera) probably originated 80–70 Ma, long after kalligrammatids became extinct. Although poor preservation of kalligrammatid fossils previously prevented their detailed morphological and ecological characterization, we examine new, well-preserved, kalligrammatid fossils from Middle Jurassic and Early Cretaceous sites in northeastern China to unravel a surprising array of similar morphological and ecological features in these two, unrelated clades. We used polarized light and epifluorescence photography, SEM imaging, energy dispersive spectrometry and time-of-flight secondary ion mass spectrometry to examine kalligrammatid fossils and their environment. We mapped the evolution of specific traits onto a kalligrammatid phylogeny and discovered that these extinct lacewings convergently evolved wing eyespots that possibly contained melanin, and wing scales, elongate tubular proboscides, similar feeding styles, and seed–plant associations, similar to butterflies. Long-proboscid kalligrammatid lacewings lived in ecosystems with gymnosperm–insect relationships and likely accessed bennettitalean pollination drops and pollen. This system later was replaced by mid-Cretaceous angiosperms and their insect pollinators.

  • 32.
    Lausmaa, Jukka
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SP – Sveriges Tekniska Forskningsinstitut / Funktionella material (KMf).
    Petronis, Sarunas
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    A novel soft tissue model for biomaterial-associated infection and inflammation - Bacteriological, morphological and molecular observations2015In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 41, p. 106–121-Article in journal (Refereed)
  • 33.
    Lausmaa, Jukka
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SP – Sveriges Tekniska Forskningsinstitut / Funktionella material (KMf).
    Stenlund, Patrik
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    A methodological study of the mechanics controlling implant fixation ex vivo2011In: Annual Conference of the European Society for Biomaterials; EBS 2011, 2011Conference paper (Other academic)
  • 34.
    Lausmaa, Jukka
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SP – Sveriges Tekniska Forskningsinstitut / Funktionella material (KMf).
    Stenlund, Patrik
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Bone response to a novel Ti-Ta-Nb-Zr alloy2015In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 20, no Jul, p. 165-175Article in journal (Refereed)
  • 35.
    Lausmaa, Jukka
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SP – Sveriges Tekniska Forskningsinstitut / Funktionella material (KMf).
    Stenlund, Patrik
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Understanding mechanisms and factors related to implant fixation; a model study of removal torque2014In: Journal of The Mechanical Behavior of Biomedical Materials, ISSN 1751-6161, E-ISSN 1878-0180, Vol. 34, no Jun, p. 83-92Article in journal (Refereed)
  • 36. Lindahl, C.
    et al.
    Xia, W.
    Engqvist, H.
    Snis, A.
    Lausmaa, Jukka
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, Göteborg, Sweden.
    Palmquist, A.
    Biomimetic calcium phosphate coating of additively manufactured porous CoCr implants2015In: Applied Surface Science, ISSN 0169-4332, E-ISSN 1873-5584, Vol. 353, p. 40-47Article in journal (Refereed)
    Abstract [en]

    The aim of this work was to study the feasibility to use a biomimetic method to prepare biomimetic hydroxyapatite (HA) coatings on CoCr substrates with short soaking times and to characterize the properties of such coatings. A second objective was to investigate if the coatings could be applied to porous CoCr implants manufactured by electron beam melting (EBM). The coating was prepared by immersing the pretreated CoCr substrates and EBM implants into the phosphate-buffered solution with Ca2+ in sealed plastic bottles, kept at 60 °C for 3 days. The formed coating was partially crystalline, slightly calcium deficient and composed of plate-like crystallites forming roundish flowers in the size range of 300-500 nm. Cross-section imaging showed a thickness of 300-500 nm. In addition, dissolution tests in Tris-HCl up to 28 days showed that a substantial amount of the coating had dissolved, however, undergoing only minor morphological changes. A uniform coating was formed within the porous network of the additive manufactured implants having similar thickness and morphology as for the flat samples. In conclusion, the present coating procedure allows coatings to be formed on CoCr and could be used for complex shaped, porous implants made by additive manufacturing.

  • 37. Lindgren, J.
    et al.
    Moyer, A.
    Schweitzer, M. H.
    Sjövall, Peter
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Uvdal, P.
    Nilsson, D. E.
    Heimdal, J.
    Engdahl, A.
    Gren, J. A.
    Schultz, B. P.
    Kear, B. P.
    Interpreting melanin-based coloration through deep time: A critical Review2015In: Proceedings of the Royal Society of London. Biological Sciences, ISSN 0962-8452, E-ISSN 1471-2954, Vol. 282, no 1813, article id 20150614Article, review/survey (Refereed)
    Abstract [en]

    Colour, derived primarily from melanin and/or carotenoid pigments, is integral to many aspects of behaviour in living vertebrates, including social signalling, sexual display and crypsis. Thus, identifying biochromes in extinct animals can shed light on the acquisition and evolution of these biological traits. Both eumelanin and melanin-containing cellular organelles (melanosomes) are preserved in fossils, but recognizing traces of ancient melanin-based coloration is fraught with interpretative ambiguity, especially when observations are based on morphological evidence alone. Assigning microbodies (or, more often reported, their ‘mouldic impressions’) as melanosome traces without adequately excluding a bacterial origin is also problematic because microbes are pervasive and intimately involved in organismal degradation. Additionally, some forms synthesize melanin. In this review, we survey both vertebrate and microbial melanization, and explore the conflicts influencing assessment of microbodies preserved in association with ancient animal soft tissues.We discuss the types of data used to interpret fossil melanosomes and evaluate whether these are sufficient for definitive diagnosis. Finally, we outline an integrated morphological and geochemical approach for detecting endogenous pigment remains and associated microstructures in multimillion-year-old fossils.

  • 38. Lindgren, J.
    et al.
    Sjövall, Peter
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Carney, R. M.
    Cincotta, A.
    Uvdal, P.
    Hutcheson, S. W.
    Gustafsson, O.
    Lefèvre, U.
    Escuillié, F.
    Heimdal, J.
    Engdahl, A.
    Gren, J. A.
    Kear, B. P.
    Wakamatsu, K.
    Yans, J.
    Godefroit, P.
    Molecular composition and ultrastructure of Jurassic paravian feathers2015In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 5, article id 13520Article in journal (Refereed)
    Abstract [en]

    Feathers are amongst the most complex epidermal structures known, and they have a well-documented evolutionary trajectory across non-avian dinosaurs and basal birds. Moreover, melanosome-like microbodies preserved in association with fossil plumage have been used to reconstruct original colour, behaviour and physiology. However, these putative ancient melanosomes might alternatively represent microorganismal residues, a conflicting interpretation compounded by a lack of unambiguous chemical data. We therefore used sensitive molecular imaging, supported by multiple independent analytical tests, to demonstrate that the filamentous epidermal appendages in a new specimen of the Jurassic paravian Anchiornis comprise remnant eumelanosomes and fibril-like microstructures, preserved as endogenous eumelanin and authigenic calcium phosphate. These results provide novel insights into the early evolution of feathers at the sub-cellular level, and unequivocally determine that melanosomes can be preserved in fossil feathers.

  • 39.
    Löwenhielm, Peter
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Dual-purpose PEG scaffolds for the preparation of soft and biofunctional hydrogels: The convergence between CuAAC and thiol-ene reactions2013In: Chemical Communications, ISSN 1359-7345, E-ISSN 1364-548X, Vol. 49, no 62, p. 6938-6940Article in journal (Refereed)
  • 40.
    Löwenhielm, Peter
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Hybrid nanoparticle arrays for measuring the interaction between cell adhesion ligands and macromolecules using SPR2011In: European Cells and Materials, ISSN 1473-2262, E-ISSN 1473-2262, Vol. 21, no S1, p. 44-Article in journal (Refereed)
  • 41.
    Löwenhielm, Peter
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Templating gold surfaces with function: A self-assembled dendritic monolayer methodology based on monodisperse polyester scaffolds2013In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 29, no 1, p. 456-465Article in journal (Refereed)
  • 42.
    Löwenhielm, Peter
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    UV initiated thiol-ene chemistry: A facile and modular synthetic methodology for the construction of functional 3D networks with tunable properties2013In: Journal of Materials Chemistry A, ISSN 2050-7488, Vol. 1, no 44, p. 13732-13737Article in journal (Refereed)
  • 43. Mahlapuu, Margit
    et al.
    Håkansson, Joakim
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Ringstad, Lovisa
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Life Science.
    Björn, Camilla
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. The Lundberg Laboratory for Diabetes Research, Department of Molecular and Clinical Medicine, The Sahlgrenska Academy at University of Gothenburg.
    Antimicrobial peptides: An emerging category of therapeutic agents2016In: Frontiers in Cellular and Infection Microbiology, Vol. 6, no DEC, article id 194Article in journal (Refereed)
    Abstract [en]

    Antimicrobial peptides (AMPs), also known as host defense peptides, are short and generally positively charged peptides found in a wide variety of life forms from microorganisms to humans. Most AMPs have the ability to kill microbial pathogens directly, whereas others act indirectly by modulating the host defense systems. Against a background of rapidly increasing resistance development to conventional antibiotics all over the world, efforts to bring AMPs into clinical use are accelerating. Several AMPs are currently being evaluated in clinical trials as novel anti-infectives, but also as new pharmacological agents to modulate the immune response, promote wound healing, and prevent post-surgical adhesions. In this review, we provide an overview of the biological role, classification, and mode of action of AMPs, discuss the opportunities and challenges to develop these peptides for clinical applications, and review the innovative formulation strategies for application of AMPs.

  • 44. Maldanis, L.
    et al.
    Carvalho, M.
    Ramos Almeida, M.
    Freitas, F. I.
    De Andrade, J. A. F. G.
    Nunes, R. S.
    Rochitte, C. E.
    Poppi, R. J.
    Freitas, R. O.
    Rodrigues, F.
    Siljeström, Sandra
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Alves Lima, F.
    Galante, D.
    Carvalho, I. S.
    Perez, C. A.
    de Carvalho, M. R.
    Bettini, J.
    Fernandez, V.
    Xavier-Neto, J.
    Heart fossilization is possible and informs the evolution of cardiac outflow tract in vertebrates2016In: eLIFE, E-ISSN 2050-084X, Vol. 5, no APRIL2016, article id e14698Article in journal (Refereed)
    Abstract [en]

    Elucidating cardiac evolution has been frustrated by lack of fossils. One celebrated enigma in cardiac evolution involves the transition from a cardiac outflow tract dominated by a Multi-Valved conus arteriosus in basal actinopterygians, to an outflow tract commanded by the Non- Valved, elastic, bulbus arteriosus in higher actinopterygians. We demonstrate that cardiac preservation is possible in the extinct fish Rhacolepis buccalis from the Brazilian Cretaceous. Using X-Ray synchrotron microtomography, we show that Rhacolepis fossils display hearts with a conus arteriosus containing at least five valve rows. This represents a transitional morphology between the primitive, multivalvar, conal condition and the derived, monovalvar, bulbar state of the outflow tract in modern actinopterygians. Our data rescue a Long-Lost cardiac phenotype (119-113 Ma) and suggest that outflow tract simplification in actinopterygians is compatible with a gradual, rather than a drastic saltation event. Overall, our results demonstrate the feasibility of studying cardiac evolution in fossils.

  • 45. Moodie, L. W. K.
    et al.
    Žužek, M.C.
    Frangež, R.
    Andersen, J. H.
    Hansen, E.
    Olsen, E. K.
    Cergolj, M.
    Sepčić, K.
    Hansen, K. Ø
    Svenson, Johan
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. Department of Chemistry, UiT the Arctic University of Norway Breivika, Tromsø, Norway.
    Synthetic analogs of stryphnusin isolated from the marine sponge: Stryphnus fortis inhibit acetylcholinesterase with no effect on muscle function or neuromuscular transmission2016In: Organic and biomolecular chemistry, ISSN 1477-0520, E-ISSN 1477-0539, Vol. 14, no 47, p. 11220-11229Article in journal (Refereed)
    Abstract [en]

    The marine secondary metabolite stryphnusin (1) was isolated from the boreal sponge Stryphnus fortis, collected off the Norwegian coast. Given its resemblance to other natural acetylcholinesterase antagonists, it was evaluated against electric eel acetylcholinesterase and displayed inhibitory activity. A library of twelve synthetic phenethylamine analogs, 2a-7a and 2b-7b, containing tertiary and quaternary amines respectively were synthesized to investigate the individual structural contributions to the activity. Compound 7b was the strongest competitive inhibitor of both acetylcholinesterase and butyrylcholinesterase with IC50 values of 57 and 20 μM, respectively. This inhibitory activity is one order of magnitude higher than the positive control physostigmine, and is comparable with several other marine acetylcholinesterase inhibitors. The physiological effect of compound 7b on muscle function and neuromuscular transmission was studied and revealed a selective mode of action at the investigated concentration. This data is of importance as the interference of therapeutic acetylcholinesterase inhibitors with neuromuscular transmission can be problematic and lead to unwanted side effects. The current findings also provide additional insights into the structure-activity relationship of both natural and synthetic acetylcholinesterase inhibitors.

  • 46.
    Oko, Asaf
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Claesson, Per M.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik. Department of Chemistry Surface and Corrosion Science, KTH Royal Institute of Technology.
    Niga, Petru
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Life Science.
    Swerin, Agne
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Measurements and dimensional scaling of spontaneous imbibition of inkjet droplets on paper2016In: Nordic Pulp & Paper Research Journal, ISSN 0283-2631, E-ISSN 2000-0669, Vol. 31, no 1, p. 156-169Article in journal (Refereed)
    Abstract [en]

    We investigate theoretically and experimentally the spontaneous imbibition of water based inkjet formulations utilizing paper capillary rise and imbibition of inkjet drops. We approximate the paper structure to a two dimensional anisotropic porous material, and using Darcy's law as a base, we derive dimensionless groups that scale drop imbibition. This derivation is based on a previous dimensional scaling of drop imbibition on thick isotropic porous material. We apply this scaling to a paper substrate by measuring the average drop imbibition rate, and perform paper capillary rise experiments to obtain the average system parameters required for the scaling. The results suggest that this approach is a valuable tool to predict drop imbibition rates on paper. We then continue and perform the same sets of experiments on a different paper with similar structure that is surface treated (surface sized) with CaCl2 salt, an additive that is known to improve print quality. We find that due to rapid aggregation of the colorant ink by the CaCl2, the imbibition rate is slowed down in the capillary rise experiments, i.e., on much larger scales compared to a single inkjet drop. However, the presence of CaCl2 has only minor effect over the average imbibition rates of single drops. Imbibition rates on the CaCl2 surface sized paper did not give adequate scaling as a result of the fact that the aggregation was not included the theoretical assumptions behind the scaling.

  • 47.
    Oko, Asaf
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Swerin, Agne
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    Infiltration and dimensional scaling of picoliter inkjet drops on nano- and microporous materials – isotropic porous glass and anisotropic paper2016In: Annual Surface and Materials Chemistry Symposium and Materials for tomorrow, ASMCS 2016, November 8-10, 2016, Gothenburg, Sweden, 2016Conference paper (Refereed)
  • 48. Olsen, E. K.
    et al.
    Hansen, E.
    Moodie, L. W. K.
    Isaksson, J.
    Sepčić, K.
    Cergolj, M.
    Svenson, Johan
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Andersen, J. H.
    Marine AChE inhibitors isolated from Geodia barretti: Natural compounds and their synthetic analogs2016In: Organic and biomolecular chemistry, ISSN 1477-0520, E-ISSN 1477-0539, Vol. 14, no 5, p. 1629-1640Article in journal (Refereed)
    Abstract [en]

    Barettin, 8,9-dihydrobarettin, bromoconicamin and a novel brominated marine indole were isolated from the boreal sponge Geodia barretti collected off the Norwegian coast. The compounds were evaluated as inhibitors of electric eel acetylcholinesterase. Barettin and 8,9-dihydrobarettin displayed significant inhibition of the enzyme, with inhibition constants (Ki) of 29 and 19 μM respectively towards acetylcholinesterase via a reversible noncompetitive mechanism. These activities are comparable to those of several other natural acetylcholinesterase inhibitors of marine origin. Bromoconicamin was less potent against acetylcholinesterase, and the novel compound was inactive. Based on the inhibitory activity, a library of 22 simplified synthetic analogs was designed and prepared to probe the role of the brominated indole, common to all the isolated compounds. From the structure-activity investigation it was shown that the brominated indole motif is not sufficient to generate a high acetylcholinesterase inhibitory activity, even when combined with natural cationic ligands for the acetylcholinesterase active site. The four natural compounds were also analysed for their butyrylcholinesterase inhibitory activity in addition and shown to display comparable activities. The study illustrates how both barettin and 8,9-dihydrobarettin display additional bioactivities which may help to explain their biological role in the producing organism. The findings also provide new insights into the structure-activity relationship of both natural and synthetic acetylcholinesterase inhibitors.

  • 49.
    Pinori, Emiliano
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik.
    Berglin, Mattias
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Material och ytteknik.
    The impact of coating hardness on the anti-barnacle efficacy of an embedded antifouling biocide2013In: Biofouling (Print), ISSN 0892-7014, E-ISSN 1029-2454, Vol. 29, no 7, p. 763-773Article in journal (Refereed)
  • 50. Shah, F. A.
    et al.
    Omar, O.
    Suska, F.
    Snis, A.
    Matic, A.
    Emanuelsson, L.
    Norlindh, B.
    Lausmaa, Jukka
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, Göteborg, Sweden.
    Thomsen, P.
    Palmquist, A.
    Long-term osseointegration of 3D printed CoCr constructs with an interconnected open-pore architecture prepared by electron beam melting2016In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 36, p. 296-309Article in journal (Refereed)
    Abstract [en]

    In orthopaedic surgery, cobalt chromium (CoCr) based alloys are used extensively for their high strength and wear properties, but with concerns over stress shielding and bone resorption due to the high stiffness of CoCr. The structural stiffness, principally related to the bulk and the elastic modulus of the material, may be lowered by appropriate design modifications, to reduce the stiffness mismatch between metal/alloy implants and the adjacent bone. Here, 3D printed CoCr and Ti6Al4V implants of similar macro-geometry and interconnected open-pore architecture prepared by electron beam melting (EBM) were evaluated following 26 week implantation in adult sheep femora. Despite higher total bone-implant contact for Ti6Al4V (39 ± 4%) than CoCr (27 ± 4%), bone formation patterns were similar, e.g., densification around the implant, and gradual ingrowth into the porous network, with more bone in the outer half (periphery) than the inner half (centre). Raman spectroscopy revealed no major differences in mineral crystallinity, the apatite-to-collagen ratio, or the carbonate-to-phosphate ratio. Energy dispersive X-ray spectroscopy showed similar Ca/P ratio of the interfacial tissue adjacent to both materials. Osteocytes made direct contact with CoCr and Ti6Al4V. While osteocyte density and distribution in the new-formed bone were largely similar for the two alloys, higher osteocyte density was observed at the periphery of the porous network for CoCr, attributable to slower remodelling and a different biomechanical environment. The results demonstrate the possibility to achieve bone ingrowth into open-pore CoCr constructs, and attest to the potential for fabricating customised osseointegrated CoCr implants for load-bearing applications. Statement of Significance Although cobalt chromium (CoCr) based alloys are used extensively in orthopaedic surgery, stress shielding due to the high stiffness of CoCr is of concern. To reduce the stiffness mismatch between CoCr and bone, CoCr and Ti6Al4V implants having an interconnected open-pore architecture were prepared by electron beam melting (EBM). After six months of submerged healing in sheep, both alloys showed similar patterns of bone formation, with densification around the implant and gradual ingrowth into the porous network. The molecular and elemental composition of the interfacial tissue was similar for both alloys. Osteocytes made direct contact with both alloys, with similar overall osteocyte density and distribution. The work attests to the potential for achieving osseointegration of EBM manufactured porous CoCr implants.

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