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  • 1.
    Eliasson, Lovisa
    et al.
    RISE., SP – Sveriges Tekniska Forskningsinstitut, SP Food and Bioscience, Processing.
    Ahrné, Lilia
    RISE., SP – Sveriges Tekniska Forskningsinstitut, SP Food and Bioscience, Processing.
    Isaksson, Sven
    RISE., SP – Sveriges Tekniska Forskningsinstitut, SP Food and Bioscience, Processing.
    Lövenklev, Maria
    RISE., SP – Sveriges Tekniska Forskningsinstitut, SP Food and Bioscience, Microbiology.
    A comparative study of infrared and microwave heating for microbial decontamination of paprika powder2015Ingår i: Frontiers in Microbiology, E-ISSN 1664-302X, Vol. 6, artikel-id 1071Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    There is currently a need in developing new decontamination technologies for spices due to limitations of existing technologies, mainly regarding their effects on spices’ sensory quality. In the search of new decontamination solutions, it is of interest to compare different technologies, to provide the industry with knowledge for taking decisions concerning appropriate decontamination technologies for spices. The present study compares infrared (IR) and microwave decontamination of naturally contaminated paprika powder after adjustment of water activity to 0.88. IR respectively microwave heating was applied to quickly heat up paprika powder to 98°C, after which the paprika sample was transferred to a conventional oven set at 98°C to keep the temperature constant during a holding time up to 20 min. In the present experimental set-up microwave treatment at 98°C for 20 min resulted in a reduction of 4.8 log units of the total number of mesophilic bacteria, while the IR treatment showed a 1 log unit lower reduction for the corresponding temperature and treatment time. Microwave and IR heating created different temperature profiles and moisture distribution within the paprika sample during the heating up part of the process, which is likely to have influenced the decontamination efficiency. The results of this study are used to discuss the difficulties in comparing two thermal technologies on equal conditions due to differences in their heating mechanisms

  • 2.
    Kölle, Martina
    et al.
    Technical University of Munich, Germany.
    Horta, Maria Augusta Crivelente
    Technical University of Munich, Germany.
    Nowrousian, Minou
    Ruhr University Bochum, Germany.
    Ohm, Robin A.
    Utrecht University, The Netherlands.
    Benz, J. Philipp
    Technical University of Munich, Germany.
    Pilgård, Annica
    RISE Research Institutes of Sweden, Bioekonomi och hälsa, Material- och ytdesign. Technical University of Munich, Germany.
    Degradative Capacity of Two Strains of Rhodonia placenta: From Phenotype to Genotype2020Ingår i: Frontiers in Microbiology, E-ISSN 1664-302X, Vol. 11, artikel-id 1338Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Brown rot fungi, such as Rhodonia placenta (previously Postia placenta), occur naturally in northern coniferous forest ecosystems and are known to be the most destructive group of decay fungi, degrading wood faster and more effectively than other wood-degrading organisms. It has been shown that brown rot fungi not only rely on enzymatic degradation of lignocellulose, but also use low molecular weight oxidative agents in a non-enzymatic degradation step prior to the enzymatic degradation. R. placenta is used in standardized decay tests in both Europe and North America. However, two different strains are employed (FPRL280 and MAD-698, respectively) for which differences in colonization-rate, mass loss, as well as in gene expression have been observed, limiting the comparability of results. To elucidate the divergence between both strains, we investigated the phenotypes in more detail and compared their genomes. Significant phenotypic differences were found between the two strains, and no fusion was possible. MAD-698 degraded scots pine more aggressively, had a more constant growth rate and produced mycelia faster than FPRL280. After sequencing the genome of FPRL280 and comparing it with the published MAD-698 genome we found 660,566 SNPs, resulting in 98.4% genome identity. Specific analysis of the carbohydrate-active enzymes, encoded by the genome (CAZome) identified differences in many families related to plant biomass degradation, including SNPs, indels, gaps or insertions within structural domains. Four genes belonging to the AA3_2 family could not be found in or amplified from FPRL280 gDNA, suggesting the absence of these genes. Differences in other CAZy encoding genes that could potentially affect the lignocellulolytic activity of the strains were also predicted by comparison of genome assemblies (e.g., GH2, GH3, GH5, GH10, GH16, GH78, GT2, GT15, and CBM13). Overall, these mutations help to explain the phenotypic differences observed between both strains as they could interfere with the enzymatic activities, substrate binding ability or protein folding. The investigation of the molecular reasons that make these two strains distinct contributes to the understanding of the development of this important brown rot reference species and will help to put the data obtained from standardized decay tests across the globe into a better biological context. © Copyright © 2020 Kölle, Horta, Nowrousian, Ohm, Benz and Pilgård.

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  • 3.
    Leekitcharoenphon, Pimlapas
    et al.
    National Food Institute, Denmark.
    Srensen, Gitte
    National Food Institute, Denmark.
    Löfström, Charlotta
    RISE - Research Institutes of Sweden, Biovetenskap och material, Jordbruk och livsmedel.
    Battisti, Antonio
    National Reference Laboratory for Antimicrobial Resistance, Italy.
    Szabo, Istvan
    Federal Institute for Risk Assessment, Germany.
    Wasyl, Dariusz
    National Veterinary Research Institute Department of Microbiology, Poland.
    Slowey, Rosemarie
    Food and the Marine Laboratories, Ireland.
    Zhao, Shaohua
    FDA, USA.
    Brisabois, Anne
    FDA, USA.
    Kornschober, Christian
    NRC Salmonella, Austrian Agency for Health and Food Safety, Austria.
    Kärssin, Age
    Veterinaar- ja Toidulaboratoorium, Estonia.
    Szilárd, Janosi
    Státní Veterinární Ústav Praha, Czech Republic.
    Cerny, Tomas
    Státní Veterinární Ústav Praha, Czech Republic.
    Svendsen, Christina Aaby
    National Food Institute, Denmark.
    Pedersen, Karl
    DTU Technical University of Denmark, Denmark.
    Aarestrup, Frank M.
    National Food Institute, Denmark.
    Hendriksen, Rene S.
    National Food Institute, Denmark.
    Cross-Border Transmission of Salmonella Choleraesuis var. Kunzendorf in European Pigs and Wild Boar: Infection, Genetics, and Evolution2019Ingår i: Frontiers in Microbiology, E-ISSN 1664-302X, Vol. 10, artikel-id 00179Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Salmonella enterica subspecies enterica serotype Choleraesuis is a swine adapted serovar. S. Choleraesuis variant Kunzendorf are responsible for the majority of outbreaks among pigs. S. Choleraesuis is rare in Europe, although there have been serious outbreaks in pigs including two outbreaks in Denmark in 1999-2000 and 2012-2013. Here, we elucidate the epidemiology, possible transmission routes and sources, and clonality of European S. Choleraesuis isolates including the Danish outbreak isolates. A total of 102 S. Choleraesuis isolates from different European countries and the United States of America, covering available isolates from the last two decades were selected for whole genome sequencing. We applied a temporally structured sequence analysis within a Bayesian framework to reconstruct a temporal and spatial phylogenetic tree. MLST type, resistance genes, plasmid replicons and accessory genes were identified using bioinformatics tools. Fifty-eight isolates including 11 out of 12 strains from wild boars were pan-susceptible. The remaining isolates carried multiple resistance genes. Eleven different plasmid replicons in eight plasmids were determined among the isolates. Accessory genes were associated to the identified resistance genes and plasmids. The European S. Choleraesuis was estimated to have emerged in 1837 (95% credible interval, 1733 - 1983) with the mutation rate of 1.02 SNPs/genome/year. The isolates were clustered according to countries and neighbour countries. There were transmission events between strains from the USA and European countries. Wild boar and pig isolates were genetically linked suggesting cross-border transmission and transmission due to a wildlife reservoir. The phylogenetic tree shows that multiple introductions were responsible for the outbreak of 2012-2013 in Denmark, and suggests that poorly disinfected vehicles crossing the border into Denmark were potentially the source of the outbreak. Low levels of single nucleotide polymorphisms (SNP) differences (0-4 SNPs) can be observed between clonal strains isolated from different organs of the same animal. Proper disinfection of livestock vehicles and improved quality control of livestock feed are recommended to prevent future spread of S. Choleraesuis or other more serious infectious diseases such as African swine fever (ASF) into the European pig production system.

  • 4.
    Salva Serra, Francisco
    et al.
    University of Gothenburg, Sweden; Sahlgrenska University Hospital,, Sweden; University of the Balearic Islands, Spain.
    Jaén-Luchoro, Daniel
    University of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden.
    Marathe, Nachiket P.
    IMR Institute of Marine Research, Norway.
    Adlerberth, Ingegerd
    University of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden.
    Moore, Edward R. B.
    University of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden.
    Karlsson, Roger
    University of Gothenburg, Sweden; Sahlgrenska University Hospital, Sweden; Nanixis Consulting, Sweden.
    Responses of carbapenemase-producing and non-producing carbapenem-resistant Pseudomonas aeruginosa strains to meropenem revealed by quantitative tandem mass spectrometry proteomics2023Ingår i: Frontiers in Microbiology, E-ISSN 1664-302X, Vol. 13Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Pseudomonas aeruginosa is an opportunistic pathogen with increasing incidence of multidrug-resistant strains, including resistance to last-resort antibiotics, such as carbapenems. Resistances are often due to complex interplays of natural and acquired resistance mechanisms that are enhanced by its large regulatory network. This study describes the proteomic responses of two carbapenem-resistant P. aeruginosa strains of high-risk clones ST235 and ST395 to subminimal inhibitory concentrations (sub-MICs) of meropenem by identifying differentially regulated proteins and pathways. Strain CCUG 51971 carries a VIM-4 metallo-β-lactamase or ‘classical’ carbapenemase; strain CCUG 70744 carries no known acquired carbapenem-resistance genes and exhibits ‘non-classical’ carbapenem-resistance. Strains were cultivated with different sub-MICs of meropenem and analyzed, using quantitative shotgun proteomics based on tandem mass tag (TMT) isobaric labeling, nano-liquid chromatography tandem-mass spectrometry and complete genome sequences. Exposure of strains to sub-MICs of meropenem resulted in hundreds of differentially regulated proteins, including β-lactamases, proteins associated with transport, peptidoglycan metabolism, cell wall organization, and regulatory proteins. Strain CCUG 51971 showed upregulation of intrinsic β-lactamases and VIM-4 carbapenemase, while CCUG 70744 exhibited a combination of upregulated intrinsic β-lactamases, efflux pumps, penicillin-binding proteins and downregulation of porins. All components of the H1 type VI secretion system were upregulated in strain CCUG 51971. Multiple metabolic pathways were affected in both strains. Sub-MICs of meropenem cause marked changes in the proteomes of carbapenem-resistant strains of P. aeruginosa exhibiting different resistance mechanisms, involving a wide range of proteins, many uncharacterized, which might play a role in the susceptibility of P. aeruginosa to meropenem.

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  • 5.
    Salva Serra, Francisco
    et al.
    University of the Balearic Islands, Spain; University of Gothenburg, Sweden.
    Pérez-Pantoja, Danilo
    Universidad Tecnológica Metropolitana, Chile.
    Donoso, Raúl A.
    Universidad Tecnológica Metropolitana, Chile; Center of Applied Ecology and Sustainability, Chile.
    Jaén-Luchoro, Daniel
    University of the Balearic Islands, Spain; University of Gothenburg, Sweden.
    Fernández-Juárez, Víctor
    University of Copenhagen, Denmark.
    Engström-Jakobsson, Hedvig
    University of Gothenburg, Sweden.
    Moore, Edward R. B.
    University of Gothenburg, Sweden.
    Lalucat, Jorge
    University of the Balearic Islands, Spain.
    Bennasar-Figueras, Antoni
    University of the Balearic Islands, Spain.
    Comparative genomics of Stutzerimonas balearica (Pseudomonas balearica): diversity, habitats, and biodegradation of aromatic compounds2023Ingår i: Frontiers in Microbiology, E-ISSN 1664-302X, Vol. 14, artikel-id 1159176Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Stutzerimonas balearica (Pseudomonas balearica) has been found principally in oil-polluted environments. The capability of S. balearica to thrive from the degradation of pollutant compounds makes it a species of interest for potential bioremediation applications. However, little has been reported about the diversity of S. balearica. In this study, genome sequences of S. balearica strains from different origins were analyzed, revealing that it is a diverse species with an open pan-genome that will continue revealing new genes and functionalities as the genomes of more strains are sequenced. The nucleotide signatures and intra- and inter-species variation of the 16S rRNA genes of S. balearica were reevaluated. A strategy of screening 16S rRNA gene sequences in public databases enabled the detection of 158 additional strains, of which only 23% were described as S. balearica. The species was detected from a wide range of environments, although mostly from aquatic and polluted environments, predominantly related to petroleum oil. Genomic and phenotypic analyses confirmed that S. balearica possesses varied inherent capabilities for aromatic compounds degradation. This study increases the knowledge of the biology and diversity of S. balearica and will serve as a basis for future work with the species.

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  • 6.
    Trigodet, Florian
    et al.
    RISE - Research Institutes of Sweden (2017-2019), Material och produktion, KIMAB. Universite Brest, France.
    Larché, Nicolas
    RISE - Research Institutes of Sweden (2017-2019), Material och produktion, KIMAB.
    Morrison, Hilary
    Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, US.
    Jebbar, Mohamed
    Universite Brest, France.
    Thierry, Dominique
    RISE - Research Institutes of Sweden (2017-2019), Material och produktion, KIMAB.
    Maignien, Lois
    Universite Brest, France; Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, US.
    Electroactive bacteria associated with stainless steel ennoblement in seawater2019Ingår i: Frontiers in Microbiology, E-ISSN 1664-302X, Vol. 10, nr FEB, artikel-id 00170Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Microorganisms can increase the open-circuit potential of stainless steel immersed in seawater of several hundred millivolts in a phenomenon called ennoblement. It raises the chance of corrosion as the open-circuit potential may go over the pitting corrosion potential. Despite the large impact of the ennoblement, no unifying mechanisms have been described as responsible for the phenomenon. Here we show that the strict electrotroph bacterium "Candidatus Tenderia electrophaga" is detected as an ennoblement biomarker and is only present at temperatures at which we observe ennoblement. This bacterium was previously enriched in biocathode systems. Our results suggest that "Candidatus Tenderia electrophaga," and its previously described extracellular electron transfer metabolism coupled to oxygen reduction activity, could play a central role in modulating stainless steel open-circuit potential and consequently mediating ennoblement.

  • 7.
    Verni, Michela
    et al.
    University of Bari Aldo Moro, Italy.
    Pontonio, Erica
    University of Bari Aldo Moro, Italy.
    Krona, Annika
    RISE Research Institutes of Sweden, Bioekonomi och hälsa, Jordbruk och livsmedel.
    Jacob, Sera
    RISE Research Institutes of Sweden.
    Pinto, Daniela
    Giuliani SpA, Italy.
    Rinaldi, Fabio
    Giuliani SpA, Italy.
    Verardo, Vito
    University of Granada, Spain.
    Díaz-de-Cerio, Elixabet
    University of Granada, Spain.
    Coda, Rossana
    University of Helsinki, Finland; Helsinki Institute of Sustainability Science, Finland.
    Rizzello, Carlo
    University of Bari Aldo Moro, Italy.
    Bioprocessing of Brewers’ Spent Grain Enhances Its Antioxidant Activity: Characterization of Phenolic Compounds and Bioactive Peptides2020Ingår i: Frontiers in Microbiology, E-ISSN 1664-302X, Vol. 11, artikel-id 1831Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Brewers’ spent grain (BSG) is the major by-product of the brewing industry which remain largely unutilized despite its nutritional quality. In this study, the effects of fermentation on BSG antioxidant potential were analyzed. A biotechnological protocol including the use of xylanase followed by fermentation with Lactiplantibacillus plantarum (Lactobacillus plantarum) PU1, PRO17, and H46 was used. Bioprocessed BSG exhibited enhanced antioxidant potential, characterized by high radical scavenging activity, long-term inhibition of linoleic acid oxidation and protective effect toward oxidative stress on human keratinocytes NCTC 2544. Immunolabelling and confocal laser microscopy showed that xylanase caused an extensive cell wall arabinoxylan disruption, contributing to the release of bound phenols molecules, thus available to further conversion through lactic acid bacteria metabolism. To clarify the role of fermentation on the antioxidant BSG potential, phenols were selectively extracted and characterized through HPLC-MS techniques. Novel antioxidant peptides were purified and identified in the most active bioprocessed BSG.

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