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  • 1. Abrahamsson, C.
    et al.
    Nordstierna, L.
    Bergenholtz, J.
    Altskär, Annika
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SIK – Institutet för livsmedel och bioteknik.
    Nyden, M.
    Magnetically induced structural anisotropy in binary colloidal gels and its effect on diffusion and pressure driven permeability2014In: Soft Matter, ISSN 1744-683X, E-ISSN 1744-6848, Vol. 10, no 24, p. 4403-4412Article in journal (Refereed)
    Abstract [en]

    We report on the synthesis, microstructure and mass transport properties of a colloidal hydrogel self-assembled from a mixture of colloidal silica and nontronite clay plates at different particle concentrations. The gel-structure had uniaxial long-range anisotropy caused by alignment of the clay particles in a strong external magnetic field. After gelation the colloidal silica covered the clay particle network, fixing the orientation of the clay plates. Comparing gels with a clay concentration between 0 and 0.7 vol%, the magnetically oriented gels had a maximum water permeability and self-diffusion coefficient at 0.3 and 0.7 vol% clay, respectively. Hence the specific clay concentration resulting in the highest liquid flux was pressure dependent. This study gives new insight into the effect of anisotropy, particle concentration and bound water on mass transport properties in nano/microporous materials. Such findings merit consideration when designing porous composite materials for use in for example fuel cell, chromatography and membrane technology. © 2014 the Partner Organisations.

  • 2. Abrahamsson, C.
    et al.
    Siahpoosh, M.
    Altskär, Annika
    SIK – Institutet för livsmedel och bioteknik.
    Nyden, M.
    Composite silica gel as test bed for flow in nano porous materials2011In: European Cells and Materials, ISSN 1473-2262, E-ISSN 1473-2262, Vol. 21, no SUPPL.1, p. 27-Article in journal (Refereed)
  • 3.
    Abrahamsson, K
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SIK – Svenska institutet för konserveringsforskning.
    Effect of various chemicals on production of toxin by Clostridium botulinum, type E1964Report (Refereed)
  • 4.
    Abrahamsson, K
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SIK – Svenska institutet för konserveringsforskning.
    Hållbarhet och kvalitet hos färska jordgubbar : en litteraturstudie1962Report (Refereed)
  • 5.
    Abrahamsson, K
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SIK – Svenska institutet för konserveringsforskning.
    Strålpastörisering av livsmedel : studievistelse i USA den 4/7-21/12 19621963Report (Refereed)
  • 6.
    Abrahamsson, K.
    SIK – Svenska institutet för konserveringsforskning.
    Studier över inverkan av olika kemikalier på toxinproduktionen hos Clostridium Botulinum typ E1964In: Nordisk Hygienisk Tidskrift, Vol. 45, no 1, p. 49-58Article in journal (Other academic)
  • 7.
    Abrahamsson, K
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SIK – Svenska institutet för konserveringsforskning.
    Studier över inverkan av olika kemikalier på toxinproduktionen hos Clostridium botulinum typ E: Studies on the effect of different chemical inhibitors on the toxinproduction of Clostridium botulinum type E1964Report (Refereed)
  • 8.
    Abrahamsson, K.
    et al.
    SIK – Svenska institutet för konserveringsforskning.
    DeSilva, N.N.
    Molin, Nils
    SIK – Svenska institutet för konserveringsforskning.
    Toxin production by Clostridium botulinum, type E, in vacuumpacked, irradiated fresh fish in relation to changes of the associated microflora1965In: Canadian Journal of Microbiology, Vol. 11, no 3, p. 523-529Article in journal (Refereed)
  • 9. Abrahamsson, K.
    et al.
    Gullmar, B.
    Molin, Nils
    SIK – Svenska institutet för konserveringsforskning.
    The effect of temperature on toxin formation and toxin stability of clostridium botulinum, type E, in different environments1966In: Canadian Journal of Microbiology, Vol. 12, no 2, p. 385-394Article in journal (Refereed)
  • 10. Acevedo, F.
    et al.
    Pizzul, Leticia
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Castillo, Maria del Pilar 
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Cuevas, R.
    Diez, M.C.
    Degradation of polycyclic aromatic hydrocarbons by the Chilean white-rot fungus Anthracophyllum discolor2011In: Journal of Hazardous Materials, ISSN 0304-3894, E-ISSN 1873-3336, Vol. 185, no 1, p. 212-219Article in journal (Refereed)
    Abstract [en]

    The degradation of three- and four-ring polycyclic aromatic hydrocarbons (PAHs) in Kirk medium by Anthracophyllum discolor, a white-rot fungus isolated from the forest of southern Chile, was evaluated. In addition, the removal efficiency of three-, four- and five-ring PAHs in contaminated soil bioaugmented with A. discolor in the absence and presence of indigenous soil microorganisms was investigated. Production of lignin-degrading enzymes and PAH mineralization in the soil were also determined. A. discolor was able to degrade PAHs in Kirk medium with the highest removal occurring in a PAH mixture, suggesting synergistic effects between PAHs or possible cometabolism. A high removal capability for phenanthrene (62%), anthracene (73%), fluoranthene (54%), pyrene (60%) and benzo(a)pyrene (75%) was observed in autoclaved soil inoculated with A. discolor in the absence of indigenous microorganisms, associated with the production of manganese peroxidase (MnP). The metabolites found in the PAH degradation were anthraquinone, phthalic acid, 4-hydroxy-9-fluorenone, 9-fluorenone and 4,5-dihydropyrene. A. discolor was able to mineralize 9% of the phenanthrene. In non-autoclaved soil, the inoculation with A. discolor did not improve the removal efficiency of PAHs. Suitable conditions must be found to promote a successful fungal bioaugmentation in non-autoclaved soils. © 2010 Elsevier B.V.

  • 11. Acevedo, F.
    et al.
    Pizzul, Leticia
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Castillo, Maria del Pilar 
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Gonzalez, M.E.
    Cea, M.
    Gianfreda, L.
    Diez, M.C.
    Degradation of polycyclic aromatic hydrocarbons by free and nanoclay-immobilized manganese peroxidase from Anthracophyllum discolor2010In: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 80, no 3, p. 271-278Article in journal (Refereed)
    Abstract [en]

    Manganese peroxidase (MnP) produced by Anthracophyllum discolor, a Chilean white rot fungus, was immobilized on nanoclay obtained from volcanic soil and its ability to degrade polycyclic aromatic hydrocarbons (PAHs) compared with the free enzyme was evaluated. At the same time, nanoclay characterization was performed.Nanoclay characterization by transmission electronic microscopy showed a particle average size smaller than 100nm. The isoelectric points (IEP) of nanoclay and MnP from A. discolor were 7.0 and 3.7, respectively, as determined by micro electrophoresis migration and preparative isoelectric focusing. Results indicated that 75% of the enzyme was immobilized on the nanoclay through physical adsorption. As compared to the free enzyme, immobilized MnP from A. discolor achieved an improved stability to temperature and pH. The activation energy (Ea) value for immobilized MnP (51.9kJmol -1) was higher than that of the free MnP (34.4kJmol -1).The immobilized enzyme was able to degrade pyrene (>86%), anthracene (>65%), alone or in mixture, and to a less extent fluoranthene (<15.2%) and phenanthrene (<8.6%). Compared to free MnP from A. discolor, the enzyme immobilized on nanoclay enhanced the enzymatic transformation of anthracene in soil.Overall results indicate that nanoclay, a carrier of natural origin, is a suitable support material for MnP immobilization. In addition, immobilized MnP shows an increased stability to high temperature, pH and time storage, as well as an enhanced PAHs degradation efficiency in soil. All these characteristics may suggest the possible use of nanoclay-immobilized MnP from A. discolor as a valuable option for in situ bioremediation purposes. © 2010 Elsevier Ltd.

  • 12. Acevedo, F.
    et al.
    Pizzul, Leticia
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Castillo, Maria del Pilar 
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Rubilar, O.
    Lienqueo, M.E.
    Tortella, G.
    Diez, M.C.
    A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp42011In: Brazilian archives of biology and technology, ISSN 1516-8913, E-ISSN 1678-4324, Vol. 54, no 6, p. 1175-1186Article in journal (Refereed)
    Abstract [en]

    In this study, different growth conditions of Anthracophyllum discolor Sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the MnP production in Kirk medium using a culture system made up of the tubes containing the glass bead. The highest MnP production (1,354 U/L on day 13) was obtained when the medium was supplemented with wheat grain and 0.25 mM MnSO 4 as inducer, under static conditions at 30°C. Two isoenzymes were purified (35 and 38 kDa respectively). MnP presented a maximal activity in the pH range between 4.5 and 5.5, a relatively high temperature tolerance (50°C) and a high catalytic activity for 2,6-dimethoxyphenol and hydrogen peroxide.

  • 13.
    Adamek, P
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SIK – Institutet för livsmedelsforskning.
    Heat resistant fungi : a literature study1996Report (Refereed)
  • 14.
    Adamek, P
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SIK – Svenska livmedelsinstitutet.
    Identifiering av mögelsvampar1986Report (Refereed)
  • 15.
    Adamek, P
    SIK – Institutet för livsmedelsforskning.
    Mögelsvampar1991Report (Refereed)
  • 16.
    Adamek, P
    RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SIK – Institutet för livsmedelsforskning.
    Mögelsvampar - en introduktion till livsmedelsmykologi1995Report (Refereed)
  • 17.
    Adamek, Petr
    SIK – Svenska livmedelsinstitutet.
    Mögelsvampen naturens egen Dr Jekyll och Mr Hyde.1988In: Livsmedelsteknik, Vol. 30, no 3, p. 70-71Article in journal (Other academic)
  • 18.
    Adolfsson, Niklas
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Arbetsrutiner för färre olyckor vid service och reparationer på jordbrukstraktorer, SLO2012Report (Refereed)
  • 19.
    Adolfsson, Niklas
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Bra arbetsmiljö lönar sig!: tips för arbetsgivare inom jordbruket, SLA2005Report (Refereed)
  • 20.
    Adolfsson, Niklas
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Enkla åtgärder för att minska vibrationsnivåer i jordbrukstraktorer, SLO 9272009Report (Refereed)
  • 21.
    Adolfsson, Niklas
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Förbättrad arbetsmiljö i silo, SLO2012Report (Refereed)
  • 22.
    Adolfsson, Niklas
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Gödsla med toalettavfall: men certifiera först!2012Report (Refereed)
  • 23.
    Adolfsson, Niklas
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Helkroppsvibrationer: Behöver lantbrukaren bry sig? SLO-8972006Report (Refereed)
  • 24.
    Adolfsson, Niklas
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Kraftöverföringsaxeln: traktorredskapets farligaste del, SLO2013Report (Refereed)
  • 25.
    Adolfsson, Niklas
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Lägre helkroppsvibrationer med en vibrationsindikator i traktorn, SLO2011Report (Refereed)
  • 26.
    Adolfsson, Niklas
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Rätt arbetsmiljö i mjölkgropen - SLO 9072008Report (Refereed)
  • 27.
    Adolfsson, Niklas
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Säkra hjulbyten på jordbrukstraktorer: i fält och i verkstad, SLO2014Report (Refereed)
  • 28.
    Adolfsson, Niklas
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Säkrare jordbruk med kamerasystem som visuellt hjälpmedel i traktorer: SLO 9462010Report (Refereed)
  • 29.
    Adolfsson, Niklas
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Geng, Qiuqing
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Exponering för olycksfallsrisk och fysisk belastning vid rid- och travskolor2008Report (Refereed)
  • 30.
    Adolfsson, Niklas
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Torén, Anna
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    JTI ger tekniktips till traktorförare2004Report (Refereed)
  • 31.
    Adolfsson, Niklas
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Torén, Anna
    Öberg, Kurt
    Biomekanisk analys av belastning i höft och skuldra vid varierande sittställning i hjullastare2002Report (Refereed)
  • 32.
    Adolfsson, Niklas
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Torén, Anna
    Öberg, Kurt
    Hälsoeffekter vid automatisk nivellering av stol vid traktorkörning2004Report (Refereed)
  • 33.
    Adolfsson, Niklas
    et al.
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Öberg, Kurt
    Torén, Anna
    Förebyggande av belastningsskador vid arbete med armstödsburna reglage i mobila arbetsmaskiner2004Report (Refereed)
  • 34. Agnihotri, S.
    et al.
    Johnsen, I.A
    Böe, M.S.
    Öyaas, Karin
    RISE, Innventia, PFI – Paper and Fiber Research Institute.
    Moe, S.
    Ethanol organosolv pretreatment of softwood (Picea abies) and sugarcane bagasse for biofuel and biorefinery applications2015In: Wood Science and Technology, ISSN 0043-7719, E-ISSN 1432-5225, Vol. 49, no 5, p. 881-896Article in journal (Refereed)
    Abstract [en]

    Ethanol derived from biomass has the potential to be a renewable transportation fuel that can replace gasoline. This work was carried out to establish an optimized ethanol organosolv pretreatment of Norway spruce (Picea abies) for bioethanol production (63 wt% EtOH, pH 3.5 in aqueous phase, 170–240 °C, 90 min) utilizing hydrolytic enzymes in the saccharification step. To test the generality of the method, a series of ethanol organosolv pretreatments were also performed on sugarcane bagasse (50 wt% EtOH, pH 3.5 in aqueous phase, 155–210 °C, 90–120 min). The degree of delignification increased with increasing temperature during pretreatment, and the fastest increase was observed with sugarcane bagasse. The pretreatments were carried out in a batch mode. The maximum degree of delignification of 65 % was reached at 235 °C for Norway spruce, while sugarcane bagasse reached 80 % at 210 °C. Cellulose was subjected to degradation (5–10 % points) at these temperatures. Subsequent enzymatic hydrolysis (30 FPU/g cellulose, 32 pNPGU/g cellulose, 50 °C, 48 h) of ethanol organosolv-pretreated biomass achieved complete conversion for both raw materials at the highest degrees of delignification.

  • 35.
    Ahlberg-Eliasson, Karin
    et al.
    Swedish Rural Economy and Agricultural Society, Sweden.
    Nadeau, Elisabet
    Swedish Rural Economy and Agricultural Society, Sweden : SLU Swedish University of Agricultural Sciences, Sweden.
    Levén, Lotta
    RISE - Research Institutes of Sweden, Bioscience and Materials, Agrifood and Bioscience.
    Schnürer, Anna
    Swedish University of Agricultural Sciences, Sweden.
    Production efficiency of Swedish farm-scale biogas plants2017In: Biomass and Bioenergy, ISSN 0961-9534, E-ISSN 1873-2909, Vol. 97, p. 27-37Article in journal (Refereed)
    Abstract [en]

    Biogas from agricultural waste streams represents an important way to produce fossil-free energy, allow nutrient recycling and reduce greenhouse gas emissions. However, biogas production from agricultural substrates is currently far from reaching its full potential. In Sweden, the number of biogas plants and their output have increased in recent years, but they are still experiencing harsh economic conditions. A recent evaluation (2010–2015) of 31 farm-scale biogas production facilities in Sweden sought to identify parameters of importance for further positive development. In this paper, data on plant operation, gas yield and digestate quality for 27 of these plants are summarised and statistically analysed to investigate factors that could allow an increase in overall biogas production and in nutrient content in the digestate. The analysis showed that addition of co-substrates to manure results in higher gas production, expressed as both specific methane potential and volumetric gas production, than when manure is the sole substrate. Use of co-substrate was also found to be influential for the nutrient content of the digestate. These observed improvements caused by co-digestion should be considered when subsidy systems for manure-based biogas processes are being created, as they could also improve the economics of biogas production. However, to achieve higher efficiency in existing biogas plants and to improve the situation for future investments, a more detailed, long-term evaluation programme should also be considered.

  • 36. Ahlgren, M.
    et al.
    Gustafsson, I.-B.
    Hall, Gunnar
    SIK – Institutet för livsmedel och bioteknik.
    Attitudes and beliefs directed towards ready meal consumption.2004In: Food Service Technology, Vol. 4, no 4, p. 159-169Article in journal (Refereed)
  • 37. Ahlgren, M.
    et al.
    Gustafsson, I.-B.
    Hall, Gunnar
    SIK – Institutet för livsmedel och bioteknik.
    Buyers' demands for ready meals: Influenced by gender and who will eat them.2006In: Journal of Foodservice, Vol. 7, p. 205-211Article in journal (Refereed)
  • 38. Ahlgren, M.
    et al.
    Gustafsson, I.-B.
    Hall, Gunnar
    SIK – Institutet för livsmedel och bioteknik.
    The impact of the meal situation on the consumption of ready meals.2005In: International Journal of Consumer Studies, Vol. 29, p. 485-492Article in journal (Refereed)
  • 39.
    Ahlgren, Mia
    SIK – Institutet för livsmedel och bioteknik.
    Ready meal consumption - when, where, why and by whom?2004Report (Other academic)
  • 40.
    Ahlgren, S
    SIK – Institutet för livsmedel och bioteknik.
    Environmental impact of chemical and mechanical weed control in agriculture : a comparing study using Life Cycle Assessment (LCA) methodology2004Report (Other academic)
  • 41. Ahlgren, S.
    et al.
    Baky, Andras
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Bernesson, S.
    Nordberg, Åke
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Norén, Olle
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Hansson, P.-A.
    Ammonium nitrate fertiliser production based on biomass: Environmental effects from a life cycle perspective2008In: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 99, no 17, p. 8034-8041Article in journal (Refereed)
    Abstract [en]

    Ammonium nitrate and calcium ammonium nitrate are the most commonly used straight nitrogen fertilisers in Europe, accounting for 43% of the total nitrogen used for fertilisers. They are both produced in a similar way; carbonate can be added as a last step to produce calcium ammonium nitrate. The environmental impact, fossil energy input and land use from using gasified biomass (cereal straw and short rotation willow (Salix) coppice) as feedstock in ammonium nitrate production were studied in a cradle-to-gate evaluation using life cycle assessment methodology. The global warming potential in the biomass systems was only 22-30% of the impact from conventional production using natural gas. The eutrophication potential was higher for the biomass systems due to nutrient leaching during cultivation, while the acidification was about the same in all systems. The primary fossil energy use was calculated to be 1.45 and 1.37 MJ/kg nitrogen for Salix and straw, respectively, compared to 35.14 MJ for natural gas. The biomass production was assumed to be self-supporting with nutrients by returning part of the ammonium nitrate produced together with the ash from the gasification. For the production of nitrogen from Salix, it was calculated that 3914 kg of nitrogen can be produced every year from 1 ha, after that 1.6% of the produced nitrogen has been returned to the Salix production. From wheat straw, 1615 kg of nitrogen can be produced annually from 1 ha, after that 0.6% of the nitrogen has been returned. © 2008 Elsevier Ltd. All rights reserved.

  • 42. Ahlgren, S.
    et al.
    Baky, Andras
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Bernesson, S.
    Nordberg, Åke
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Norén, Olle
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Hansson, P.-A.
    Future fuel supply systems for organic production based on Fischer-Tropsch diesel and dimethyl ether from on-farm-grown biomass2008In: Biosystems Engineering, ISSN 1537-5110, E-ISSN 1537-5129, Vol. 99, no 1, p. 145-155Article in journal (Refereed)
    Abstract [en]

    The effects of making a 1000 ha organic farm self-sufficient in renewable fuel were studied. Biomass grown on-farm can be transported to large fuel production facilities and the fuel transported back to the farm. Two fuels, Fischer-Tropsch diesel (FTD) and dimethyl ether (DME), produced from either straw or short-rotation willow coppice (Salix), were studied. The environmental impact, land use and energy balance were calculated using life-cycle methodology. It was calculated that the straw-based systems had only 32-39% of the impact on global warming (kg [CO2-eq]) compared to the Salix-based systems. For acidification and eutrophication, the differences between the systems were less significant. The energy balances were 8.9 and 9.6 for FTD and 10.1 and 10.0 for DME, from straw and Salix, respectively. To become self-sufficient in FTD, 108 ha has to be set aside for Salix production or 261 ha of straw collected from the existing crop rotation. For DME the corresponding figures are 38 and 70 ha. The many by-products in the FTD scenarios explain the large difference between fuels. Comparing FTD and DME, the differences in environmental impact were small. Considering this, FTD is a more likely alternative since DME requires a pressurised infrastructure system and engine modifications. © 2007 IAgrE.

  • 43. Ahlgren, S.
    et al.
    Baky, Andras
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Bernesson, S.
    Nordberg, Åke
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Norén, Olle
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Hansson, P.-A.
    Tractive power in organic farming based on fuel cell technology: Energy balance and environmental load2009In: Agricultural Systems, ISSN 0308-521X, E-ISSN 1873-2267, Vol. 102, no 1-3, p. 67-76Article in journal (Refereed)
    Abstract [en]

    This study analysed a future hypothetical organic farm self-sufficient in renewable tractor fuel. Biomass from the farm was assumed to be transported to a central fuel production plant and the fuel returned to the farm, where it was utilised in fuel cell powered tractors. The land use, energy balance and environmental impact of five different scenarios were studied. In the first two scenarios, straw was used as raw material for production of hydrogen or methanol via thermochemical gasification. In the third and fourth scenarios, short rotation forest (Salix) was used as raw material for the same fuels. In the fifth scenario, ley was used as raw material for hydrogen fuel via biogas production. The straw scenarios had the lowest impact in all studied environmental impact categories since the Salix scenarios had higher soil emissions and the ley scenario had comparatively large emissions from the fuel production. The energy balance was also favourable for straw, 16.3 and 19.5 for hydrogen and methanol respectively, compared to Salix 14.2 and 15.6. For ley to hydrogen the energy balance was only 6.1 due to low efficiency in the fuel production. In the Salix scenarios, 1.6% and 2.0% of the land was set aside for raw material production in the hydrogen and methanol scenarios respectively. In the straw scenarios no land needed to be reserved, but straw was collected on 4.3% and 5.3% of the area for hydrogen and methanol respectively. To produce hydrogen from ley, 4% of the land was harvested. The study showed that the difference in environmental performance lay in choice of raw material rather than choice of fuel. Hydrogen is a gas with low volumetric energy density, which requires an adapted infrastructure and tractors equipped with gas tanks. This leads to the conclusion that methanol probably will be the preferred choice if a fuel cell powered farm would be put into practice in the future. © 2009 Elsevier Ltd. All rights reserved.

  • 44. Ahlgren, S.
    et al.
    Bernesson, S.
    Nordberg, Åke
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Hansson, P-A.
    Nitrogen fertiliser production based on biogas: Energy input, environmental impact and land use.2010In: Bioresource Technology, Vol. 101, p. 7192-7195Article in journal (Refereed)
  • 45. Ahlgren, Serina
    et al.
    Baky, Andras
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Bernesson, Sven
    Nordberg, Åke
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Det svenska jordbrukets framtida drivmedelsförsörjning2010Report (Refereed)
  • 46. Ahlgren, Serina
    et al.
    Baky, Andras
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Bernesson, Sven
    Nordberg, Åke
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Green nitrogen2011Report (Refereed)
  • 47. Ahlgren, Serina
    et al.
    Baky, Andras
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Bernesson, Sven
    Nordberg, Åke
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Norén, Olle
    RISE, SP – Sveriges Tekniska Forskningsinstitut, JTI Institutet för Jordbruks- och Miljöteknik.
    Hansson, Per-Anders
    Consequential life cycle assessment of nitrogen fertilisers based on biomass: A Swedish perspective.2012In: Insciences Journal Climate Change, Vol. 2, no 4, p. 80-101Article in journal (Refereed)
  • 48. Ahlqvist, J.
    et al.
    Dainiak, M.B.
    Kumar, A.
    Hornsten, Gunnar E.
    Galaev, I.Yu.
    Mattiasson, B.
    Monitoring the production of inclusion bodies during fermentation and enzyme-linked immunosorbent assay analysis of intact inclusion bodies using cryogel minicolumn plates2006In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 354, no 2, p. 229-237Article in journal (Refereed)
    Abstract [en]

    A novel minicolumn chromatographic method to monitor the production of inclusion bodies during fermentation and an enzyme-linked immunosorbent assay (ELISA) system allowing direct analysis of the particles with surface-displayed antigens are described. A 33-kDa protein containing 306 amino acids with three sulfur bridges produced as inclusion bodies was labeled with polyclonal antibodies against 15 amino acid (anti-A15) and 17 amino acid (anti-B17) residues at the N- and C-terminal ends of the protein, respectively. Labeled particles were bound to macroporous monolithic protein A-cryogel adsorbents inserted into the open-ended wells of a 96-well plate (referred to as protein A-cryogel minicolumn plate). The concept behind this application is that the binding degree of inclusion bodies from lysed fermentation broth to the cryogel minicolumns increases with an increase in their concentration during fermentation. The technique allowed us to monitor the increase in the production levels of the inclusion bodies as the fermentation process progressed. The system also has a built-in quality parameter to ensure that the target protein has been fully expressed. Alternatively, inclusion bodies immobilized on phenyl-cryogel minicolumn plate were used in indirect ELISA based on anti-A15 and anti-B17 antibodies against terminal amino acid residues displayed on the surface of inclusion bodies. Drainage-protected properties of the cryogel minicolumns allow performance of successive reactions with tested immunoglobulin G (IgG) samples and enzyme-conjugated secondary IgG and of enzymatic reaction within the adsorbent. © 2006 Elsevier Inc. All rights reserved.

  • 49. Ahlqvist, J.
    et al.
    Kumar, A.
    Sundstrom, H.
    Ledung, E.
    Hornsten, E.G.
    Enfors, S.-O.
    Affinity binding of inclusion bodies on supermacroporous monolithic cryogels using labeling with specific antibodies2006In: Journal of Biotechnology, ISSN 0168-1656, E-ISSN 1873-4863, Vol. 122, no 2, p. 216-225Article in journal (Refereed)
    Abstract [en]

    A new chromatographic method based on affinity supermacroporous monolithic cryogels is developed for binding and analyzing inclusion bodies during fermentation. The work demonstrated that it is possible to bind specific IgG and IgY antibodies to the 15 and 17 amino acids at the terminus ends of a 33 kDa target protein aggregated as inclusion bodies. The antibody treated inclusion bodies from lysed fermentation broth can be specifically retained in protein A and pseudo-biospecific ligand sulfamethazine modified supermacroporous cryogels. The degree of binding of IgG and IgY treated inclusion bodies to the Protein A and sulfamethazine gels are investigated, as well as the influence of pH on the sulfamethazine ligand. Optimum binding of 78 and 72% was observed on both protein A and sulfamethazine modified cryogel columns, respectively, using IgG labeling of the inclusion bodies. The antibody treated inclusion bodies pass through unretained in the sulfamethazine supermacroporous gel at pH that does not favour the binding between the ligand on the gel and the antibodies on the surface of inclusion bodies. Also the unlabeled inclusion bodies went through the gel unretained, showing no non-specific binding or trapping within the gel. These findings may very well be the foundation for the building of a powerful analytical tool during fermentation of inclusion bodies as well as a convenient way to purify them from fermentation broth. These results also support our earlier findings [Kumar, A., Plieva, F.M., Galaev, I.Yu., Mattiasson, B., 2003. Affinity fractionation of lymphocytes using a monolithic cyogel. J. Immunol. Methods 283, 185-194] with mammalian cells that were surface labeled with specific antibodies and recognized on protein A supermacroporous gels. A general binding and separation system can be established on antibody binding cryogel affinity matrices. © 2005 Elsevier B.V. All rights reserved.

  • 50.
    Ahlqvist, Josefin
    SIK – Institutet för livsmedel och bioteknik.
    Using monolithic cryogels for direct capture of inclusion bodies during fermentation - a way to monitor the process2006Report (Refereed)
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