The vibrational response of wooden floor systems is an issue that needs to be dealt with more adequately. Notably, studies addressing human response to vibrations are needed in order to better estimate what level of vibrations in dwellings can be seen as acceptable. In this investigation, measurements on five different floors were performed in a laboratory environment at two locations in Sweden. Acceleration measurements were carried out while a person either was walking on a particular floor or was seated in a chair placed there, as the test leader was walking on the floor. These participants filled out a questionnaire regarding their perception and experiencing of the vibrations. Independently of the subjective tests, acceleration measurements were also carried out, using a shaker as excitation source, with the aim of determining the dynamic characteristics of the floors. In addition, static load tests were performed using displacement gauges so as to measure the floor deflections. The ultimate aim was to develop indicators of human response to floor vibrations, specifically those regarding vibration acceptability and vibration annoyance, their being drawn based on relationships between the questionnaire responses obtained and the parameter values determined on the basis of the measurements carried out.