In residential multi-storey buildings of timber it is of great importance to reduce the flanking transmission of noise. Some building systems do this by installing a vibration-damping elastic interlayer, Sylomer® or Sylodyn®, in the junction between the support and the floor structure. This interlayer also improves the floor vibration performance by adding damping to the structure. In the present work the vibration performance of a floor with such interlayers has been investigated both in laboratory and field tests. A prefabricated timber floor element was tested in laboratory on rigid supports and on supports with four different types of interlayers. The results are compared with in situ tests on a copy of the same floor element. The effect on vibration performance i.e. frequencies, damping ratio and mode shapes is studied. A comparison of the in situ test and the test with elastic interlayer in laboratory shows that the damping in situ is approximately three times higher than on a single floor element in the lab. This indicates that the damping in situ is affected be the surrounding building structure. The achieved damping ratio is highly dependent on the mode shapes. Mode shapes that have high mode shape coefficients along the edges where the interlayer material is located, result in higher modal damping ratios. The impulse velocity response, that is used to evaluate the vibration performance and rate experienced annoyance in the design of wooden joist floors, seems to be reduced when adding elastic layers at the supports.