Wood nanocellulose is a novel biomaterial for wound dressing applications. Wood nanocellulose was produced from never-dried P. radiata pulp fibres. The applied pre-treatment was 2,2,6,6-tetramethylpiperidinyl-1-oxyl  (TEMPO) mediated oxidation. To characterise bacterial growth, P. aeruginosa PAO1 biofilms were grown in Mueller Hinton broth on air-dried films. Various microscopy techniques, including atomic force microscopy (AFM), confocal laser scanning microscopy (CLSM) and field-emission scanning electron microscopy (FESEM), were applied to characterise the nanocellulose material and the bacterial-nanocellulose interactions.   Multiscale assessments, including FESEM and AFM, revealed the effective fibrillation of the fibre wall structure, yielding nanofibrils with diameters less than 20 nm and lengths in the micrometre-scale. Importantly, we have demonstrated that the growth of PAO1 was inhibited in the presence of the nanocellulose suspensions when compared to the control. Additionally, SEM imaging revealed distinct clusters of PAO1 cells growing on the surfaces of nanocellulose films. This work highlights the potential usefulness of novel nanocellulose materials in wound dressings with optimized characteristics.