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Comparison between methods using low-toxicity solvents for the extraction of lipids from herring (Clupea harengus)
SIK – Institutet för livsmedel och bioteknik.
SIK – Institutet för livsmedel och bioteknik.
SIK – Institutet för livsmedel och bioteknik.
1998 (English)In: Food chemistry, ISSN 0346-718X, Vol. 61, no 3, 355-365 p.Article in journal (Refereed)
Abstract [en]

Three alkane/alcohol/water-based lipid extraction systems were evaluated to determine which would be the best replacement for the frequently used chloroform/methanol water system, nowadays known to be very toxic. All the methods were applied to samples of minced herring (Clupea harengus) differing in quality and composition. In addition to comparisons of total lipid yield, the extracted lipids were compared with respect to content of triglycerides, phospholipids, free fatty acids, ?-tocopherol, lipid hydroperoxides and conjugated dienes. The content of phospholipids was found to differ most between the lipids extracted by the four methods. Here, the chloroform methanol water system was the most efficient, followed by heptane ethanol water/sodium dodecyl sulphate (SDS) and then iso-propanol hexane. However, by decreasing the level of SDS, the efficiency of the heptane/ethanol/water SDS system in extracting phospholipids was increased to the same level as that of the chloroform/methanol/water system. This decrease in SDS also resulted in a higher recovery of free fatty acids. The lack of correlation between yields of phospholipids and yields of lipid oxidation products throughout this study was surprising because of the often-described susceptibility of phospholipids to oxidation.

Place, publisher, year, edition, pages
1998. Vol. 61, no 3, 355-365 p.
Keyword [en]
Food Engineering
Keyword [sv]
Livsmedelsteknik
National Category
Food Science
Identifiers
URN: urn:nbn:se:ri:diva-8574OAI: oai:DiVA.org:ri-8574DiVA: diva2:966446
Available from: 2016-09-08 Created: 2016-09-08Bibliographically approved

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SIK – Institutet för livsmedel och bioteknik
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CiteExportLink to record
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  • apa
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