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Effects of amylopectin structure and molecular weight on microstructural and rheological properties of mixed beta-lactoglobulin gels
SIK – Institutet för livsmedel och bioteknik.
2003 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 4, no 5, 1400-1409 p.Article in journal (Refereed)
Abstract [en]

Nongelling amylopectin fractions from potato and barley have been used to form mixed ?-lactoglobulin gels. The amylopectin fractions were produced by varying the time of ?-amylase hydrolysis followed by sequential ethanol precipitation. The molecular weights, radius of gyration, chain length distribution, and viscosity of the fractions were established. The mixed gels were analyzed theologically with dynamic mechanical analysis in shear and microstructurally with light microscopy, transmission electron microscopy, and nuclear magnetic resonance spectroscopy. The result of the gel studies clearly showed that small differences in the molecular weight of amylopectins have a significant influence on the kinetics of protein aggregation and thereby on the gel microstructure and the rheological behavior of the gel. Both an increase in the molecular weight and a higher concentration of amylopectins resulted in a more open protein network structure, with thicker strands of larger and more close-packed ?-lactoglobulin clusters, which showed a larger storage modulus. The transmission electron micrographs revealed that degraded amylopectins were enclosed inside the protein clusters in the mixed gels, whereas nondegraded amylopectin was only found outside the protein clusters. The volume-weighted mean value of the molecular weight of the amylopectins was found to vary between 3.2 × 10 4 and 5.0 × 10 7 Da and the ratio of gyration between 14 and 61 nm. The maximum in chain length distribution was generally somewhat distributed toward longer chain lengths for potato compared to barley, but the differences in chain length distribution were minor compared to those seen in the molecular weight and ratio of gyration between the fractions.

Place, publisher, year, edition, pages
2003. Vol. 4, no 5, 1400-1409 p.
Keyword [en]
Food Engineering
Keyword [sv]
Livsmedelsteknik
National Category
Food Science
Identifiers
URN: urn:nbn:se:ri:diva-8396DOI: 10.1021/bm030038ePubMedID: 12959612OAI: oai:DiVA.org:ri-8396DiVA: diva2:966267
Available from: 2016-09-08 Created: 2016-09-08Bibliographically approved

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