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Protein biochips patterned by microcontact printing or by adsorption-soft lithography in two modes
SIK – Institutet för livsmedel och bioteknik.
2008 (English)In: Biointerphases, ISSN 1934-8630, E-ISSN 1559-4106, Vol. 3, no 3, 75-82 p.Article in journal (Refereed)
Abstract [en]

Patterning of proteins is critical to protein biochips. Printing of layers of proteins is well established, as is adsorption of proteins to surfaces properly modified with surface chemical functionalities. The authors show that simple methods based on soft lithography stamps can be used to prepare functional antibody chips through both these routes. Both methods incorporate transfer of the stamp material poly(dimethylsiloxane) (PDMS) to the biochip, whether intended or not intended. The results indicate that microcontact printing of proteins always includes PDMS transfer, thereby creating a possibility of unspecific adsorption to a hydrophobic domain. © 2008 American Vacuum Society.

Place, publisher, year, edition, pages
2008. Vol. 3, no 3, 75-82 p.
Keyword [en]
Food Engineering
Keyword [sv]
Livsmedelsteknik
National Category
Food Science
Identifiers
URN: urn:nbn:se:ri:diva-8386DOI: 10.1116/1.2988771OAI: oai:DiVA.org:ri-8386DiVA: diva2:966257
Available from: 2016-09-08 Created: 2016-09-08Bibliographically approved

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