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Studying solutions at high shear rates: A dedicated microfluidics setup
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2016 (English)In: Journal of Synchrotron Radiation, ISSN 0909-0495, E-ISSN 1600-5775, Vol. 23, no 2, 480-486 p.Article in journal (Refereed) Published
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Abstract [en]

The development of a dedicated small-angle X-ray scattering setup for the investigation of complex fluids at different controlled shear conditions is reported. The setup utilizes a microfluidics chip with a narrowing channel. As a consequence, a shear gradient is generated within the channel and the effect of shear rate on structure and interactions is mapped spatially. In a first experiment small-angle X-ray scattering is utilized to investigate highly concentrated protein solutions up to a shear rate of 300000 s-1. These data demonstrate that equilibrium clusters of lysozyme are destabilized at high shear rates.

Place, publisher, year, edition, pages
International Union of Crystallography , 2016. Vol. 23, no 2, 480-486 p.
Keyword [en]
aggregation, beamline setup, complex solutions, lysozyme, microfluidics, protein interaction, rheology, shear, small-angle X-ray scattering (SAXS)
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Physical Chemistry Other Chemistry Topics
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URN: urn:nbn:se:ri:diva-128DOI: 10.1107/S1600577515024856Scopus ID: 2-s2.0-84959274343OAI: oai:DiVA.org:ri-128DiVA: diva2:933951
Available from: 2016-06-07 Created: 2016-05-31 Last updated: 2017-11-30Bibliographically approved

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