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Interplay between flow and diffusion in capillary alginate hydrogels
RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Food and Bioscience, Structure Design.
RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Food and Bioscience, Structure Design.
RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Food and Bioscience, Structure Design.
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2016 (English)In: Soft Matter, Vol. 12, no 17, 3897-3907 p.Article in journal (Refereed) Published
Abstract [en]

Alginate gels with naturally occurring macroscopic capillaries have been used as a model system to study the interplay between laminar flow and diffusion of nanometer-sized solutes in real time. Calcium alginate gels that contain homogeneously distributed parallel-aligned capillary structures were formed by external addition of crosslinking ions to an alginate sol. The effects of different flow rates (0, 1, 10, 50 and 100 μl min-1) and three different probes (fluorescein, 10 kDa and 500 kDa fluorescein isothiocyanate-dextran) on the diffusion rates of the solutes across the capillary wall and in the bulk gel in between the capillaries were investigated using confocal laser scanning microscopy. The flow in the capillaries was produced using a syringe pump that was connected to the capillaries via a tube. Transmission electron microscopy revealed an open aggregated structure close to the capillary wall, followed by an aligned network layer and the isotropic network of the bulk gel. The most pronounced effect was observed for the 1 nm-diameter fluorescein probe, for which an increase in flow rate increased the mobility of the probe in the gel. Fluorescence recovery after photobleaching confirmed increased mobility close to the channel, with increasing flow rate. Mobility maps derived using raster image correlation spectroscopy showed that the layer with the lowest mobility corresponded to the anisotropic layer of ordered network chains. The combination of microscopy techniques used in the present study elucidates the flow and diffusion behaviors visually, qualitatively and quantitatively, and represents a promising tool for future studies of mass transport in non-equilibrium systems. © 2016 The Royal Society of Chemistry.

Place, publisher, year, edition, pages
Royal Society of Chemistry , 2016. Vol. 12, no 17, 3897-3907 p.
Keyword [en]
Alginate, Capillarity, Diffusion, Gels, High resolution transmission electron microscopy, Laminar flow, Photobleaching, Probes, Sols, Transmission electron microscopy, Aggregated structure, Calcium alginate gels, Capillary structures, Confocal laser scanning microscopy, Fluorescein isothiocyanate dextrans, Fluorescence recovery after photobleaching, Microscopy technique, Nonequilibrium system, Network layers
National Category
Food Science
Identifiers
URN: urn:nbn:se:ri:diva-132ISBN: 1744683X (ISSN) (print)OAI: oai:DiVA.org:ri-132DiVA: diva2:932829
Available from: 2016-06-02 Created: 2016-06-02 Last updated: 2017-02-06Bibliographically approved

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Lorén, N.

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