Determination of the chemical composition of biomaterial is important for their valued utilization in biorefinery. In this study, the chemical composition of seven wood species, i.e. lambu (Khaya anthotheca), raj-koroi (Albizia richardiana), jhau (Casuarina equisetifolia), sil-koroi (Albizia procera), katbadam (Terminalia catappa), jolpai (Elaeocarpus robustus), and arjun (Terminalia arjuna) were examined. The chemical characterization of these wood species can expedite a further study on the extraction of cellulose, lignin, and extractive. alpha-cellulose content was in the range of 37.0% to 42.1% and lignin content was 20.4% to 34.1%. The solubility in 1% caustic soda was 16.1% to 24.3%. The a-cellulose and lignin content were similar to other wood species. Therefore, these species can be a potential source of raw material for biorefinery.