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Multicolor Fluorescent Labeling of Cellulose Nanofibrils by Click Chemistry
Stockholm University, Sweden.
Stockholm University, Sweden.
Stockholm University, Sweden.
Stockholm University, Sweden.ORCID iD: 0000-0002-9816-5270
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2015 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 16, no 4, p. 1293-1300Article in journal (Refereed) Published
Abstract [en]

(Figure Presented) We have chemically modified cellulose nanofibrils (CNF) with furan and maleimide groups, and selectively labeled the modified CNF with fluorescent probes; 7-mercapto-4-methylcoumarin and fluorescein diacetate 5-maleimide, through two specific click chemistry reactions: Diels-Alder cycloaddition and the thiol-Michael reaction. Characterization by solid-state 13C NMR and infrared spectroscopy was used to follow the surface modification and estimate the substitution degrees. We demonstrate that the two luminescent dyes could be selectively labeled onto CNF, yielding a multicolor CNF that was characterized by UV/visible and fluorescence spectroscopies. It was demonstrated that the multicolor CNF could be imaged using a confocal laser scanning microscope. 
Place, publisher, year, edition, pages
American Chemical Society , 2015. Vol. 16, no 4, p. 1293-1300
Keywords [en]
Addition reactions; Cellulose; Cycloaddition; Fluorescence; Fluorescence spectroscopy; Infrared spectroscopy; Nanofibers; Synthesis (chemical), Cellulose nanofibrils; Chemically modified; Confocal laser scanning microscope; Diels-Alder cycloadditions; Fluorescein diacetate; Fluorescent labeling; Fluorescent probes; Substitution degree, Functional groups, cellulose; coumarin; fluorescein derivative; fluorescein diacetate; fluorescent dye; furan; maleimide; nanofiber; cellulose; fluorescent dye; furan derivative; maleimide derivative; nanoparticle, Article; carbon nuclear magnetic resonance; chromatophore; click chemistry; confocal laser microscopy; cycloaddition; Diels Alder reaction; fluorescence spectroscopy; infrared spectroscopy; Michael addition; priority journal; ultraviolet radiation; chemistry; click chemistry; procedures; wood, Alnus, Cellulose; Click Chemistry; Fluorescent Dyes; Furans; Maleimides; Nanoparticles; Wood
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Materials Engineering
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URN: urn:nbn:se:ri:diva-68239DOI: 10.1021/acs.biomac.5b00083Scopus ID: 2-s2.0-84927950232OAI: oai:DiVA.org:ri-68239DiVA, id: diva2:1817387
Available from: 2023-12-06 Created: 2023-12-06 Last updated: 2024-01-03Bibliographically approved

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Fall, Andreas

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