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Insight into the assembly of lipid-hyaluronan complexes in osteoarthritic conditions
University of Illinois at Urbana-Champaign, USA.
Oak Ridge National Laboratory, USA.
RISE Research Institutes of Sweden, Bioeconomy and Health, Material and Surface Design. KTH Royal Institute of Technology, Sweden; University of New South Wales, Australia; École Centrale de Lyon, France.ORCID iD: 0000-0002-8935-8070
University of Tennessee, USA.
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2023 (English)In: Biointerphases, ISSN 1934-8630, E-ISSN 1559-4106, Vol. 18, no 2, article id 021005Article in journal (Refereed) Published
Abstract [en]

Interactions between molecules in the synovial fluid and the cartilage surface may play a vital role in the formation of adsorbed films that contribute to the low friction of cartilage boundary lubrication. Osteoarthritis (OA) is the most common degenerative joint disease. Previous studies have shown that in OA-diseased joints, hyaluronan (HA) not only breaks down resulting in a much lower molecular weight (MW), but also its concentration is reduced ten times. Here, we have investigated the structural changes of lipid-HA complexes as a function of HA concentration and MW to simulate the physiologically relevant conditions that exist in healthy and diseased joints. Small angle neutron scattering and dynamic light scattering were used to determine the structure of HA-lipid vesicles in bulk solution, while a combination of atomic force microscopy and quartz crystal microbalance was applied to study their assembly on a gold surface. We infer a significant influence of both MW and HA concentrations on the structure of HA-lipid complexes in bulk and assembled on a gold surface. Our results suggest that low MW HA cannot form an amorphous layer on the gold surface, which is expected to negatively impact the mechanical integrity and longevity of the boundary layer and could contribute to the increased wear of the cartilage that has been reported in joints diseased with OA. © 2023 Author(s).

Place, publisher, year, edition, pages
American Institute of Physics Inc. , 2023. Vol. 18, no 2, article id 021005
Keywords [en]
Gold, Hyaluronic acid, Joints (anatomy), Light scattering, Neutron scattering, Adsorbed films, Boundary lubrications, Cartilage surfaces, Condition, Diseased joints, Gold surfaces, Hyaluronan, Low friction, Low molecular weight, Synovial fluid, Cartilage, dipalmitoylphosphatidylcholine, animal, chemistry, Gallus gallus, human, molecular dynamics, molecular weight, osteoarthritis, pathology, 1, 2-Dipalmitoylphosphatidylcholine, Animals, Chickens, Humans, Molecular Dynamics Simulation
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:ri:diva-64393DOI: 10.1116/6.0002502Scopus ID: 2-s2.0-85152291967OAI: oai:DiVA.org:ri-64393DiVA, id: diva2:1754361
Note

 Funding details: National Science Foundation, NSF, CMMI 17-61696, CMMI 20-35122, CMMI 21-21681; Funding text 1: This article is based on work supported by the National Science Foundation under Grant Nos. CMMI 17-61696, CMMI 20-35122, and CMMI 21-21681. The research was carried out in part in the Materials Research Laboratory Central Research Facilities, University of Illinois. This research used resources at the Spallation Neutron Source, a DOE Office of Science User Facility operated by the Oak Ridge National Laboratory.

Available from: 2023-05-03 Created: 2023-05-03 Last updated: 2023-05-25Bibliographically approved

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Rutland, Mark W.

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