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Inclusion complex formation between sulfadiazine and various modified β-cyclodextrins and characterization of the complexes
University Grenoble Alpes, France.
RISE Research Institutes of Sweden, Bioeconomy and Health, Material and Surface Design.ORCID iD: 0000-0001-8876-8898
RISE Research Institutes of Sweden, Bioeconomy and Health, Material and Surface Design. NTNU, Norway.ORCID iD: 0000-0003-2271-3637
University Grenoble Alpes, France.
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2022 (English)In: Journal of Drug Delivery Science and Technology, ISSN 1773-2247, Vol. 76, article id 103814Article in journal (Refereed) Published
Abstract [en]

β-Cyclodextrin (β-CD) and its derivatives are cyclic oligosaccharides which present the ability to form inclusion complexes with hydrophobic molecules and can bring new functionalities to a wide range of materials. As of today, the most used prophylactic drugs for wound dressing applications are sulfadiazine (SD) and its derivatives silver sulfadiazine (SSD). These drugs are used to prevent infections of the wounds; however, their low intrinsic water-solubility is a hindrance to their use. In this study, the inclusion complex formation between SD/SSD and the various β-CDs were assessed with various protocols. Isothermal Titration Calorimetry (ITC) experiments led to the conclusion that the formation constants measured for SD and SSD are sufficiently similar meaning that SD can be considered as a satisfactory model molecule. Phase Solubility Diagram (PSD) were built for SD and the various β-CDs, highlighting a 1:1 stoichiometry of inclusion and a linear increase in solubility of SD with increasing concentration of β-CDs- The formation constant ranged from 197 M−1 to 245 M−1 for the different β-CDs. X-Ray diffraction (XRD) and Differential Scanning Calorimetry (DSC) experiments revealed the different physico-chemical properties affected by the formation of an inclusion complex. Finally, Nuclear Magnetic Resonance (NMR) experiments confirmed the depth of penetration of SD inside the β-CDs cavity as well as the orientation of SD, highlighting the fact that CM-β-CDs induce a deeper penetration than other β-CDs.

Place, publisher, year, edition, pages
Editions de Sante , 2022. Vol. 76, article id 103814
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:ri:diva-63089DOI: 10.1016/j.jddst.2022.103814Scopus ID: 2-s2.0-85142698857OAI: oai:DiVA.org:ri-63089DiVA, id: diva2:1730755
Note

 Funding details: Norges Teknisk-Naturvitenskapelige Universitet, NTNU; Funding details: Agence Nationale de la Recherche, ANR, ANR-15-IDEX-02; Funding details: Labex, ANR-11-LABX-0030; Funding details: Department of Chemical Engineering, Universiti Teknologi Petronas; Funding details: European Regional Development Fund, ERDF; Funding details: Région Auvergne-Rhône-Alpes; Funding details: Institut Carnot PolyNat, ANR-16-CARN-0025-01; Funding text 1: This work is supported by the French National Research Agency in the framework of the “Investissements d'avenir” program Glyco@Alps (ANR-15-IDEX-02) and NTNU through its Department of Chemical Engineering. LGP2 is part of the LabEx Tec 21 (Investissements d'Avenir—Grant Agreement No. ANR-11-LABX-0030) and of the PolyNat Carnot Institute (Investissements d'Avenir—Grant Agreement No. ANR-16-CARN-0025-01).This research was made possible thanks to the facilities of the TekLiCell platform funded by the Région Rhône-Alpes (ERDF: European regional development fund). E. Gillon and A. Imberty (Cermav) for lab support with ITC, I. Jeacommine (Cermav) for NMR measurements and T. Encinas (CMTC) for XRD measurements.; Funding text 2: This work is supported by the French National Research Agency in the framework of the “Investissements d'avenir” program Glyco@Alps ( ANR-15-IDEX-02 ) and NTNU through its Department of Chemical Engineering. LGP2 is part of the LabEx Tec 21 (Investissements d’Avenir—Grant Agreement No. ANR-11-LABX-0030 ) and of the PolyNat Carnot Institute (Investissements d’Avenir—Grant Agreement No. ANR-16-CARN-0025-01 ).This research was made possible thanks to the facilities of the TekLiCell platform funded by the Région Rhône-Alpes (ERDF: European regional development fund) . E. Gillon and A. Imberty (Cermav) for lab support with ITC, I. Jeacommine (Cermav) for NMR measurements and T. Encinas (CMTC) for XRD measurements.

Available from: 2023-01-25 Created: 2023-01-25 Last updated: 2023-05-25Bibliographically approved

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Heggset, Ellinor BSyverud, Kristin

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