This study compares several forest biomass-based biofuels and some electrofuels, for use in cars and trucks, in terms of economic and climate performance and resource efficiency from a Swedish perspective. Both dropin fuels possible to blend in conventional fuels and single molecule fuels requiring new vehicles and infrastructure are included. Mature costs for feedstock, production, distribution, and vehicles are included. There is no clear winner between drop-in and single-molecular fuels when considering both costs, GHG emissions and resource efficiency, neither for cars nor trucks. For trucks, both single-molecular fuels in the form of methanol and DME (dimethyl ether) and drop-in fuels in the form of diesel based on lignin and from hydropyrolysis perform best (given a process designed to reach high GHG performance). For cars drop-in fuels such as petrol produced from lignin or hydropyrolysis perform well, closely followed by the single molecular fuels methanol, DME and methane and some of the other drop-in fuels. For cars, where electrification is progressing fast, it is reasonable to apply the drop-in fuel strategy. For trucks, either continue with the drop-in fuel strategy or, due to uncertainties linked to new fuel production processes, invest in single molecule fuels such as methanol and DME.