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Efficient Delivery of Hydrophilic Small Molecules to Retinal Cell Lines Using Gel Core-Containing Solid Lipid Nanoparticles
University of Modena and Reggio Emilia, Italy.
InoCure sro, Czech Republic.ORCID iD: 0000-0002-1610-458X
University of Tuebingen, Germany.
RISE Research Institutes of Sweden, Bioeconomy and Health, Chemical Process and Pharmaceutical Development.ORCID iD: 0000-0002-4941-5915
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2022 (English)In: Pharmaceutics, E-ISSN 1999-4923, Vol. 14, no 1, p. 74-74Article in journal (Refereed) Published
Abstract [en]

In this study, we developed a novel solid lipid nanoparticle (SLN) formulation for drug delivery of small hydrophilic cargos to the retina. The new formulation, based on a gel core and composite shell, allowed up to two-fold increase in the encapsulation efficiency. The type of hydrophobic polyester used in the composite shell mixture affected the particle surface charge, colloidal stability, and cell internalization profile. We validated SLNs as a drug delivery system by performing the encapsulation of a hydrophilic neuroprotective cyclic guanosine monophosphate analog, previously demonstrated to hold retinoprotective properties, and the best formulation resulted in particles with a size of ±250 nm, anionic charge > −20 mV, and an encapsulation efficiency of ±60%, criteria that are suitable for retinal delivery. In vitro studies using the ARPE-19 and 661W retinal cell lines revealed the relatively low toxicity of SLNs, even when a high particle concentration was used. More importantly, SLN could be taken up by the cells and the release of the hydrophilic cargo in the cytoplasm was visually demonstrated. These findings suggest that the newly developed SLN with a gel core and composite polymer/lipid shell holds all the characteristics suitable for the drug delivery of small hydrophilic active molecules into retinal cells.

Place, publisher, year, edition, pages
2022. Vol. 14, no 1, p. 74-74
Keywords [en]
drug delivery system; thermoresponsive polymer; rod photoreceptor; retinal pigment epithelium
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:ri:diva-58475DOI: 10.3390/pharmaceutics14010074OAI: oai:DiVA.org:ri-58475DiVA, id: diva2:1634072
Funder
European Commission, H2020-MSCA-ITN-765441Available from: 2022-02-01 Created: 2022-02-01 Last updated: 2024-07-04Bibliographically approved

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Perez, OswaldoSchipper, Nicolaas

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