Anti-colonization effect of au surfaces with self-assembled molecular monolayers functionalized with antimicrobial peptides on s. EpidermidisShow others and affiliations
2021 (English)In: Antibiotics, E-ISSN 2079-6382, Vol. 10, no 12, article id 1516
Article in journal (Refereed) Published
Abstract [en]
Medical devices with an effective anti-colonization surface are important tools for com-batting healthcare-associated infections. Here, we investigated the anti-colonization efficacy of antimicrobial peptides covalently attached to a gold model surface. The gold surface was modified by a self-assembled polyethylene glycol monolayer with an acetylene terminus. The peptides were covalently connected to the surface through a copper-catalyzed [3 + 2] azide-acetylene coupling (CuAAC). The anti-colonization efficacy of the surfaces varied as a function of the antimicrobial activity of the peptides, and very effective surfaces could be prepared with a 6 log unit reduction in bacterial colonization. © 2021 by the authors.
Place, publisher, year, edition, pages
MDPI , 2021. Vol. 10, no 12, article id 1516
Keywords [en]
Anti-colonization, Antifouling, Antimicrobial peptide, Antimicrobial surface, Certika, Self-assembled monolayer, ToF-SIMS imaging, acetylene, copper, dichloromethane, gold, macrogol, macrogol 200, macrogol 400, phenylalanine, polypeptide antibiotic agent, self assembled monolayer, tryptophan, antibiotic sensitivity, antimicrobial activity, Article, bacterial colonization, colony forming unit, column chromatography, drug synthesis, electrospray mass spectrometry, Escherichia coli, Gram negative bacterium, healthcare associated infection, high performance liquid chromatography, lipophilicity, minimum inhibitory concentration, nonhuman, proton nuclear magnetic resonance, Staphylococcus epidermidis, time of flight mass spectrometry
National Category
Biomaterials Science
Identifiers
URN: urn:nbn:se:ri:diva-57900DOI: 10.3390/antibiotics10121516Scopus ID: 2-s2.0-85121753149OAI: oai:DiVA.org:ri-57900DiVA, id: diva2:1626020
Note
Funding details: Norges Forskningsråd, 283272; Funding text 1: This research was funded by Amicoat AS and the Research Council of Norway, grant number 283272.
2022-01-102022-01-102024-07-04Bibliographically approved