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Development of stable vibrio cholerae O1 Hikojima type vaccine strains co-expressing the Inaba and Ogawa lipopolysaccharide antigens
University of Gothenburg, Sweden.ORCID iD: 0000-0001-6783-4622
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2014 (English)In: PLOS ONE, E-ISSN 1932-6203, Vol. 9, no 11, article id e108521Article in journal (Refereed) Published
Abstract [en]

We describe here the development of stable classical and El Tor V. cholerae O1 strains of the Hikojima serotype that co-express the Inaba and Ogawa antigens of O1 lipopolysaccharide (LPS). Mutation of the wbeTgene reduced LPS perosamine methylation and thereby gave only partial transformation into Ogawa LPS on the cell surface. The strains express approximately equal amounts of Inaba-and Ogawa-LPS antigens which are preserved after formalin-inactivation of the bacteria. Oral immunizations of both inbred and outbred mice with formalin-inactivated whole-cell vaccine preparations of these strains elicited strong intestinal IgA anti-LPS as well as serum vibriocidal antibody responses against both Inaba and Ogawa that were fully comparable to the responses induced by the licensed Dukoral vaccine. Passive protection studies in infant mice showed that immune sera raised against either of the novel Hikojima vaccine strains protected baby mice against infection with virulent strains of both serotypes. This study illustrates the power of using genetic manipulation to improve the properties of bacteria strains for use in killed whole-cell vaccines.

Place, publisher, year, edition, pages
Public Library of Science , 2014. Vol. 9, no 11, article id e108521
Keywords [en]
bacterial antigen, bacterium lipopolysaccharide, cholera vaccine, formaldehyde, immunoglobulin A antibody, immunoglobulin M antibody, Inaba antigen, Ogawa antigen, unclassified drug, antiserum, immunoglobulin A, inactivated vaccine, lipopolysaccharide, O antigen, adult, animal experiment, animal model, animal tissue, antibody blood level, antibody response, antibody specificity, antibody titer, Article, bacterial gene, bacterial strain, cholera, controlled study, cross reaction, female, gene mutation, genetic manipulation, genetic stability, immunogenicity, infant, infection prevention, mouse, nonhuman, site directed mutagenesis, survival rate, Vibrio cholerae, wbeT gene, animal, antibody production, classification, genetics, immunity, immunology, intestine mucosa, metabolism, mutagenesis, plasmid, serotyping, Vibrio cholerae O1, Animals, Antibody Formation, Cholera Vaccines, Cross Reactions, Genes, Bacterial, Immune Sera, Intestinal Mucosa, Lipopolysaccharides, Mice, O Antigens, Plasmids, Vaccines, Inactivated
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-56874DOI: 10.1371/journal.pone.0108521Scopus ID: 2-s2.0-84915745692OAI: oai:DiVA.org:ri-56874DiVA, id: diva2:1612607
Available from: 2021-11-18 Created: 2021-11-18 Last updated: 2021-11-18Bibliographically approved

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Nygren, Erik

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