Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Diversity and abundance of microbial communities in uasb reactors during methane production from hydrolyzed wheat straw and lucerne
SLU Swedish University of Agricultural Science, Sweden.
SLU Swedish University of Agricultural Science, Sweden.
RISE Research Institutes of Sweden, Built Environment, Energy and Resources.ORCID iD: 0000-0002-5758-4137
RISE Research Institutes of Sweden, Built Environment, Energy and Resources.ORCID iD: 0000-0002-0359-3652
Show others and affiliations
2020 (English)In: Microorganisms, E-ISSN 2076-2607, Vol. 8, no 9, article id 1394Article in journal (Refereed) Published
Abstract [en]

The use of straw for biofuel production is encouraged by the European Union. A previous study showed the feasibility of producing biomethane in upflow anaerobic sludge blanket (UASB) reactors using hydrolyzed, steam-pretreated wheat straw, before and after dark fermentation with Caldicellulosiruptor saccharolyticus, and lucerne. This study provides information on overall microbial community development in those UASB processes and changes related to acidification. The bacterial and archaeal community in granular samples was analyzed using high-throughput amplicon sequencing. Anaerobic digestion model no. 1 (ADM1) was used to predict the abundance of microbial functional groups. The sequencing results showed decreased richness and diversity in the microbial community, and decreased relative abundance of bacteria in relation to archaea, after process acidification. Canonical correspondence analysis showed significant negative correlations between the concentration of organic acids and three phyla, and positive correlations with seven phyla. Organic loading rate and total COD fed also showed significant correlations with microbial community structure, which changed over time. ADM1 predicted a decrease in acetate degraders after a decrease to pH ≤ 6.5. Acidification had a sustained effect on the microbial community and process performance. © 2020 by the authors.

Place, publisher, year, edition, pages
MDPI AG , 2020. Vol. 8, no 9, article id 1394
Keywords [en]
ADM1, Methane production, Microbial community, Next-generation amplicon sequencing, Process acidification, UASB, VFA, Wheat straw hydrolysate
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-48927DOI: 10.3390/microorganisms8091394Scopus ID: 2-s2.0-85091379987OAI: oai:DiVA.org:ri-48927DiVA, id: diva2:1476453
Note

Funding details: Energimyndigheten; Funding details: Svenska ForskningsrÃ¥det Formas, 2017-00795; Funding details: Energimyndigheten, 31090-2; Funding details: Lunds Universitet; Funding text 1: Funding: This research was funded by the Swedish Energy Agency (Energimyndigheten), grant number 31090-2. E.K. was also funded by the Division of Biotechnology, Department of Chemistry, Lund University. K.W. was also funded by the Swedish Research Council for Sustainable Development, FORMAS (project no. 2017-00795). The APC was funded by Lund University.

Available from: 2020-10-14 Created: 2020-10-14 Last updated: 2021-02-04Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textScopus

Authority records

Björkmalm, JohannaWillquist, Karin

Search in DiVA

By author/editor
Björkmalm, JohannaWillquist, Karin
By organisation
Energy and Resources
In the same journal
Microorganisms
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 43 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf