Diversity and abundance of microbial communities in uasb reactors during methane production from hydrolyzed wheat straw and lucerneShow others and affiliations
2020 (English)In: Microorganisms, E-ISSN 2076-2607, Vol. 8, no 9, article id 1394Article in journal (Refereed) Published
Abstract [en]
The use of straw for biofuel production is encouraged by the European Union. A previous study showed the feasibility of producing biomethane in upflow anaerobic sludge blanket (UASB) reactors using hydrolyzed, steam-pretreated wheat straw, before and after dark fermentation with Caldicellulosiruptor saccharolyticus, and lucerne. This study provides information on overall microbial community development in those UASB processes and changes related to acidification. The bacterial and archaeal community in granular samples was analyzed using high-throughput amplicon sequencing. Anaerobic digestion model no. 1 (ADM1) was used to predict the abundance of microbial functional groups. The sequencing results showed decreased richness and diversity in the microbial community, and decreased relative abundance of bacteria in relation to archaea, after process acidification. Canonical correspondence analysis showed significant negative correlations between the concentration of organic acids and three phyla, and positive correlations with seven phyla. Organic loading rate and total COD fed also showed significant correlations with microbial community structure, which changed over time. ADM1 predicted a decrease in acetate degraders after a decrease to pH ≤ 6.5. Acidification had a sustained effect on the microbial community and process performance. © 2020 by the authors.
Place, publisher, year, edition, pages
MDPI AG , 2020. Vol. 8, no 9, article id 1394
Keywords [en]
ADM1, Methane production, Microbial community, Next-generation amplicon sequencing, Process acidification, UASB, VFA, Wheat straw hydrolysate
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-48927DOI: 10.3390/microorganisms8091394Scopus ID: 2-s2.0-85091379987OAI: oai:DiVA.org:ri-48927DiVA, id: diva2:1476453
Note
Funding details: Energimyndigheten; Funding details: Svenska Forskningsrådet Formas, 2017-00795; Funding details: Energimyndigheten, 31090-2; Funding details: Lunds Universitet; Funding text 1: Funding: This research was funded by the Swedish Energy Agency (Energimyndigheten), grant number 31090-2. E.K. was also funded by the Division of Biotechnology, Department of Chemistry, Lund University. K.W. was also funded by the Swedish Research Council for Sustainable Development, FORMAS (project no. 2017-00795). The APC was funded by Lund University.
2020-10-142020-10-142021-02-04Bibliographically approved