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Influence of the Molecular Weight and the Presence of Calcium Ions on the Molecular Interaction of Hyaluronan and DPPC
Institute of Materials Research, Germany.
Institute of Materials Research, Germany.
RISE Research Institutes of Sweden. KTH Royal Institute of Technology, Sweden.
RISE Research Institutes of Sweden. KTH Royal Institute of Technology, Sweden.
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2020 (English)In: Molecules, ISSN 1431-5157, E-ISSN 1420-3049, Vol. 25, no 17, article id 3907Article in journal (Refereed) Published
Abstract [en]

Hyaluronan is an essential physiological bio macromolecule with different functions. One prominent area is the synovial fluid which exhibits remarkable lubrication properties. However, the synovial fluid is a multi-component system where different macromolecules interact in a synergetic fashion. Within this study we focus on the interaction of hyaluronan and phospholipids, which are thought to play a key role for lubrication. We investigate how the interactions and the association structures formed by hyaluronan (HA) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) are influenced by the molecular weight of the bio polymer and the ionic composition of the solution. We combine techniques allowing us to investigate the phase behavior of lipids (differential scanning calorimetry, zeta potential and electrophoretic mobility) with structural investigation (dynamic light scattering, small angle scattering) and theoretical simulations (molecular dynamics). The interaction of hyaluronan and phospholipids depends on the molecular weight, where hyaluronan with lower molecular weight has the strongest interaction. Furthermore, the interaction is increased by the presence of calcium ions. Our simulations show that calcium ions are located close to the carboxylate groups of HA and, by this, reduce the number of formed hydrogen bonds between HA and DPPC. The observed change in the DPPC phase behavior can be attributed to a local charge inversion by calcium ions binding to the carboxylate groups as the binding distribution of hyaluronan and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine is not changed.

Place, publisher, year, edition, pages
NLM (Medline) , 2020. Vol. 25, no 17, article id 3907
Keywords [en]
binding distribution, hyaluronan, molecular interaction, phase behavior, phospholipid
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-48356DOI: 10.3390/molecules25173907Scopus ID: 2-s2.0-85090106202OAI: oai:DiVA.org:ri-48356DiVA, id: diva2:1466059
Available from: 2020-09-10 Created: 2020-09-10 Last updated: 2023-08-28Bibliographically approved

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