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Single-use printed biosensor for l-lactate and its application in bioprocess monitoring
RISE Research Institutes of Sweden.
Linköping University, Sweden.
Technische Universität Berlin, Germany.
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2020 (English)In: Processes, ISSN 2227-9717, Vol. 8, no 3, article id 321Article in journal (Refereed) Published
Abstract [en]

There is a profound need in bioprocess manufacturing for low-cost single-use sensors that allow timely monitoring of critical product and production attributes. One such opportunity is screen-printed enzyme-based electrochemical sensors, which have the potential to enable low-cost online and/or off-line monitoring of specific parameters in bioprocesses. In this study, such a singleuse electrochemical biosensor for lactate monitoring is designed and evaluated. Several aspects of its fabrication and use are addressed, including enzyme immobilization, stability, shelf-life and reproducibility. Applicability of the biosensor to off-line monitoring of bioprocesses was shown by testing in two common industrial bioprocesses in which lactate is a critical quality attribute (Corynebacterium fermentation and mammalian Chinese hamster ovary (CHO) cell cultivation). The specific response to lactate of the screen-printed biosensor was characterized by amperometric measurements. The usability of the sensor at typical industrial culture conditions was favorably evaluated and benchmarked with commonly used standard methods (HPLC and enzymatic kits). The single-use biosensor allowed fast and accurate detection of lactate in prediluted culture media used in industrial practice. The design and fabrication of the biosensor could most likely be adapted to several other critical bioprocess analytes using other specific enzymes. This makes this single-use screen-printed biosensor concept a potentially interesting and versatile tool for further applications in bioprocess monitoring. © 2020 by the authors.

Place, publisher, year, edition, pages
MDPI AG , 2020. Vol. 8, no 3, article id 321
Keywords [en]
At-line measurement, Enzyme electrode, In-line monitoring, Lactate biosensor, Off-line monitoring, Screen-printing
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-44692DOI: 10.3390/pr8030321Scopus ID: 2-s2.0-85081985509OAI: oai:DiVA.org:ri-44692DiVA, id: diva2:1417812
Note

Funding details: 643056; Funding text 1: This project has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sk?odowska-Curie actions grant agreement No. 643056 (BIORAPID). The authors also thank Fujifilm Diosynth Biotechnologies for providing the CHO cell line and the culture media formulation.

Available from: 2020-03-30 Created: 2020-03-30 Last updated: 2020-03-30Bibliographically approved

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Beni, Valerio

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