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Concentrated protein solutions investigated using acoustic levitation and small-Angle X-ray scattering
DTU Technical University of Denmark, Denmark.
RISE Research Institutes of Sweden, Bioeconomy and Health, Chemical Process and Pharmaceutical Development. Lund University, Sweden.
University of Copenhagen, Denmark.
DTU Technical University of Denmark, Denmark.
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2020 (English)In: Journal of Synchrotron Radiation, ISSN 0909-0495, E-ISSN 1600-5775, Vol. 27, p. 396-404Article in journal (Refereed) Published
Abstract [en]

An acoustically levitated droplet has been used to collect synchrotron SAXS data on human serum albumin protein solutions up to a protein concentration of 400mgml-1. A careful selection of experiments allows for fast data collection of a large amount of data, spanning a protein concentration/solvent concentration space with limited sample consumption (down to 3μL per experiment) and few measurements. The data analysis shows data of high quality that are reproducible and comparable with data from standard flow-Through capillary-based experiments. Furthermore, using this methodology, it is possible to achieve concentrations that would not be accessible by conventional cells. The protein concentration and ionic strength parameter space diagram may be covered easily and the amount of protein sample is significantly reduced (by a factor of 100 in this work). Used in routine measurements, the benefits in terms of protein cost and time spent are very significant. 

Place, publisher, year, edition, pages
International Union of Crystallography , 2020. Vol. 27, p. 396-404
Keywords [en]
concentrated protein solutions, levitated droplet, SAXS, structure factors, Data acquisition, Drops, Ionic strength, X ray scattering, Acoustic levitation, Acoustically levitated droplet, Human serum albumins, Protein concentrations, Protein solution, Proteins, article, human, human tissue, X ray crystallography
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-44693DOI: 10.1107/S1600577519016977Scopus ID: 2-s2.0-85081892819OAI: oai:DiVA.org:ri-44693DiVA, id: diva2:1417808
Note

Export Date: 30 March 2020; Article

Available from: 2020-03-30 Created: 2020-03-30 Last updated: 2020-03-30Bibliographically approved

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