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Surface Functionalization of PTFE Membranes Intended for Guided Bone Regeneration Using Recombinant Spider Silk
KTH Royal Institute of Technology, Sweden.
RISE Research Institutes of Sweden, Materials and Production, Chemistry, Biomaterials and Textiles.ORCID iD: 0000-0003-4592-5851
Neoss Ltd, Sweden.
KTH Royal Institute of Technology, Sweden.
2020 (English)In: ACS Applied Bio Materials, ISSN 2576-6422, Vol. 3, no 1, p. 577-583Article in journal (Refereed) Published
Abstract [en]

Alveolar bone loss is usually treated with guided bone regeneration, a dental procedure which utilizes a tissue-separation membrane. The barrier membrane prevents pathogens and epithelial cells to invade the bone augmentation site, thereby permitting osteoblasts to deposit minerals and build up bone. This study aims at adding bioactive properties to otherwise inert PTFE membranes in order to enhance cell adherence and promote proliferation. A prewetting by ethanol and stepwise hydration protocol was herein employed to overcome high surface tension of PTFE membranes and allow for a recombinant spider silk protein, functionalized with a cell-binding motif from fibronectin (FN-silk), to self-assemble into a nanofibrillar coating. HaCaT and U-2 OS cells were seeded onto soft and hard tissue sides, respectively, of membranes coated with FN-silk. The cells could firmly adhere as early as 1 h post seeding, as well as markedly grow in numbers when kept in culture for 7 days. Fluorescence and scanning electron microscopy images revealed that adherent cells could form a confluent monolayer and develop essential cell-cell contacts during 1 week of culture. Hence, functionalized PTFE membranes have a potential of better integration at the implantation site, with reduced risk of membrane displacement as well as exposure to oral pathogens.

Place, publisher, year, edition, pages
American Chemical Society , 2020. Vol. 3, no 1, p. 577-583
Keywords [en]
cell adherence, guided bone regeneration, nonreinforced PTFE membranes, recombinant spider silk, surface functionalization, Calcium compounds, Cell adhesion, Cells, Membranes, Recombinant proteins, Scanning electron microscopy, Tissue, Tissue regeneration, PTFE membranes, Spider silks, Bone
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-43943DOI: 10.1021/acsabm.9b00972Scopus ID: 2-s2.0-85078675977OAI: oai:DiVA.org:ri-43943DiVA, id: diva2:1395693
Note

Funding details: VINNOVA; Funding details: VetenskapsrÃ¥det, VR; Funding text 1: This research was supported by Vinnova, Swedish Research Council and Knut & Alice Wallenberg foundation.

Available from: 2020-02-24 Created: 2020-02-24 Last updated: 2020-02-24Bibliographically approved

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Petronis, Sarunas

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