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Fully Automated Microsystem for Unmediated Electrochemical Characterization, Visualization and Monitoring of Bacteria on Solid Media; E. coli K-12: A Case Study.
Universidad de los Andes, Colombia.
RISE - Research Institutes of Sweden (2017-2019), ICT, Acreo. Linköping University, Sweden.ORCID iD: 0000-0001-6889-0351
Universidad de los Andes, Colombia.
2019 (English)In: Biosensors, ISSN 2079-6374, Vol. 9, no 4, article id E131Article in journal (Refereed) Published
Abstract [en]

In this paper, we present a non-fluidic microsystem for the simultaneous visualization and electrochemical evaluation of confined, growing bacteria on solid media. Using a completely automated platform, real-time monitoring of bacterial and image-based computer characterization of growth were performed. Electrochemical tests, using Escherichia coli K-12 as the model microorganism, revealed the development of a faradaic process at the bacteria-microelectrode interface inside the microsystem, as implied by cyclic voltammetry and electrochemical impedance spectrometry measurements. The electrochemical information was used to determine the moment in which bacteria colonized the electrode-enabled area of the microsystem. This microsystem shows potential advantages for long-term electrochemical monitoring of the extracellular environment of cell culture and has been designed using readily available technologies that can be easily integrated in routine protocols. Complementarily, these methods can help elucidate fundamental questions of the electron transfer of bacterial cultures and are potentially feasible to be integrated into current characterization techniques.

Place, publisher, year, edition, pages
2019. Vol. 9, no 4, article id E131
Keywords [en]
automated system, bacteria, bio-electrochemical systems, cyclic voltammetry, direct electron transfer, electrochemical impedance spectrometry, microscopy, microsystem
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-42538DOI: 10.3390/bios9040131PubMedID: 31689950Scopus ID: 2-s2.0-85074741651OAI: oai:DiVA.org:ri-42538DiVA, id: diva2:1384664
Available from: 2020-01-10 Created: 2020-01-10 Last updated: 2020-02-07Bibliographically approved

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Beni, Valerio

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