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A Miniature Graphene-based Biosensor for Intracellular Glucose Measurements
Linköping University, Sweden; bCentres of Excellence in Science and Applied Technologies, Pakistan.
Linköping University, Sweden; COMSATS Institute of Information Technology, Pakistan.
COMSATS Institute of Information Technology, Pakistan.
RISE, Swedish ICT, Acreo. RISE - Research Institutes of Sweden, ICT, Acreo.ORCID iD: 0000-0002-0631-3804
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2015 (English)In: Electrochimica Acta, ISSN 0013-4686, E-ISSN 1873-3859, Vol. 174, p. 574-580Article in journal (Refereed) Published
Abstract [en]

We report on a small and simple graphene-based potentiometric sensor for the measurement of intracellular glucose concentration. A fine borosilicate glass capillary coated with graphene and subsequently immobilized with glucose oxidase (GOD) enzyme is inserted into the intracellular environment of a single human cell. The functional groups on the edge plane of graphene assist the attachment with the free amine terminals of GOD enzyme, resulting in a better immobilization. The sensor exhibits a glucose-dependent electrochemical potential against an Ag/AgCl reference microelectrode which is linear across the whole concentration range of interest (10 - 1000 μM). Glucose concentration in human fat cell measured by our graphene-based sensor is in good agreement with nuclear magnetic resonance (NMR) spectroscopy. 

Place, publisher, year, edition, pages
Elsevier Ltd , 2015. Vol. 174, p. 574-580
Keywords [en]
Bio-sensors, Glucose Sensor, Glucose-oxidase, Graphene, Graphene oxide, Intracellular sensors, Biosensors, Borosilicate glass, Enzyme immobilization, Enzymes, Functional groups, Glucose, Glucose oxidase, Glucose sensors, Microelectrodes, Nuclear magnetic resonance, Nuclear magnetic resonance spectroscopy, Borosilicate glass capillary, Electrochemical potential, Graphene oxides, Graphene-based sensors, Intracellular glucose concentration, Intracellular glucose measurement, Intracellular sensor, Nuclear magnetic resonance(NMR)
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Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-42047DOI: 10.1016/j.electacta.2015.06.035Scopus ID: 2-s2.0-84934937944OAI: oai:DiVA.org:ri-42047DiVA, id: diva2:1378579
Available from: 2019-12-13 Created: 2019-12-13 Last updated: 2019-12-13Bibliographically approved

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Sandberg, Mats

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