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Diazonium-based impedimetric aptasensor for the rapid label-free detection of Salmonella typhimurium in food sample
Linkoping University, Sweden; University of Mazandaran, Iran.
University of Mazandaran, Iran.
Linkoping University, Sweden.
Linkoping University, Sweden.
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2016 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 80, p. 566-573Article in journal (Refereed) Published
Abstract [en]

Fast and accurate detection of microorganisms is of key importance in clinical analysis and in food and water quality monitoring. Salmonella typhimurium is responsible for about a third of all cases of foodborne diseases and consequently, its fast detection is of great importance for ensuring the safety of foodstuffs.We report the development of a label-free impedimetric aptamer-based biosensor for S. typhimurium detection. The aptamer biosensor was fabricated by grafting a diazonium-supporting layer onto screen-printed carbon electrodes (SPEs), via electrochemical or chemical approaches, followed by chemical immobilisation of aminated-aptamer. FTIR-ATR, contact angle and electrochemical measurements were used to monitor the fabrication process. Results showed that electrochemical immobilisation of the diazonium-grafting layer allowed the formation of a denser aptamer layer, which resulted in higher sensitivity. The developed aptamer-biosensor responded linearly, on a logarithm scale, over the concentration range 1×101 to 1×108 CFU mL-1, with a limit of quantification (LOQ) of 1×101 CFU mL-1 and a limit of detection (LOD) of 6 CFU mL-1. Selectivity studies showed that the aptamer biosensor could discriminate S. typhimurium from 6 other model bacteria strains. Finally, recovery studies demonstrated its suitability for the detection of S. typhimurium in spiked (1×102, 1×104 and 1×106 CFU mL-1) apple juice samples.

Place, publisher, year, edition, pages
Elsevier Ltd , 2016. Vol. 80, p. 566-573
Keywords [en]
Aptamer, Diazonium grafting, Electrochemical impedance spectroscopy, Food analysis, Label-free detection, S. typhimurium, Biosensors, Carbon, Electrodes, Food microbiology, Fourier transform infrared spectroscopy, Fruit juices, Quality control, Salmonella, Water quality, Aptamers, Grafting (chemical), outer membrane protein, diazonium compound, Article, bacterial strain, bioluminescence, biosensor, colony forming unit, controlled study, diazonium based impedimetric aptasensor, electrochemistry, limit of detection, limit of quantitation, monolayer culture, nonhuman, optical density, potentiometry, real time polymerase chain reaction, Salmonella enterica serovar Typhimurium, chemistry, electrochemical analysis, food control, genetic procedures, human, isolation and purification, pathogenicity, Aptamers, Nucleotide, Biosensing Techniques, Diazonium Compounds, Electrochemical Techniques, Humans, Salmonella typhimurium
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-42014DOI: 10.1016/j.bios.2016.02.024Scopus ID: 2-s2.0-84958212322OAI: oai:DiVA.org:ri-42014DiVA, id: diva2:1378547
Note

Funding details: Ministry of Science Research and Technology; Funding text 1: ZB acknowledges the Ministry of Science Research and Technology of Iran ( www.msrt.ir ) to support her study visit at Linköping University. The authors acknowledge Vetenskapsrådet (Pathoscreen project; Swedish Research Link; ref.-ID: D0675001) for supporting the development of the aptasensor and Dr. V. C. Ozalp for helping with the aptamer identification.

Available from: 2019-12-13 Created: 2019-12-13 Last updated: 2019-12-13Bibliographically approved

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Beni, Valerio

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