Fiber pull-out is generally considered to be the dominating failure mechanism in fiber reinforced concrete (FRC). Accordingly, pull-out tests are typically performed to characterize the fiber-matrix interaction. However, little direct insight can be gained on the actual mechanisms ofthe pull-out from such a test. Deeper understanding could however be gained through the addition of non-destructive techniques to pull-out tests to enable the visualization and quantification of the mechanical interaction. Pull-out mechanisms for different common steel fibers were investigated using adapted pull-out tests performed in-situ in an X-ray micro tomography (µXRT). High resolution volume images from the µXRT scans enable clear visualization of aggregates, pores, fiber and fiber-matrix interface. Furthermore, the natural density speckle pattern from aggregate distribution and pores was found to be suitable for Digital Volume Correlation (DVC) analysis. From the DVC results it was possible to visualize and quantify the strain distribution in the matrix around the fiber at different load levels up to final failure, being marked by either pull-out or fiber rupture. The load transfer mechanism was initially dominated by shear along the fiber. As the load increased, slip occurred in the end-hook region and mechanical locking became the governing mechanism. This study demonstrates that strain measurements within the concrete matrix and passive end-slip can be obtained successfully using µXRT imaging and DVC analysis, which leads to an increased understanding of the interaction mechanisms in fiber reinforced concrete under mechanical loading.