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Fluorescence-based real-time quantitative polymerase chain reaction (qPCR) technologies for high throughput screening of pathogens
DTU Technical University of Denmark, Denmark.ORCID iD: 0000-0001-6492-1245
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2014 (English)In: High Throughput Screening for Food Safety Assessment: Biosensor Technologies, Hyperspectral Imaging and Practical Applications, Elsevier Inc. , 2014, p. 219-248Chapter in book (Other academic)
Abstract [en]

Real-time quantitative polymerase chain reaction (qPCR) technology is being widely used for high throughput diagnostics of pathogens in the food production chain as an alternative to the more time-consuming and laborious culture-based standard methods. This chapter discusses the basics of qPCR, including data analysis, PCR instruments, and detection chemistries. The importance of choosing appropriate pre-PCR processing strategies - i.e., sampling, sample preparation, and PCR chemistry - to avoid PCR inhibition and achieve correct quantification is furthermore included. Major novel trends such as portable PCR instruments for in-field diagnostics, high-resolution melting curve analysis, and digital PCR are addressed. Finally, some perspectives on future trends are given. © 2015 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
Elsevier Inc. , 2014. p. 219-248
Keywords [en]
Detection, Food production chain, Food safety, Foodborne pathogens, Real-time quantitative PCR, Chains, Error detection, Food microbiology, Importance sampling, Throughput, Food production, Food-borne pathogens, High throughput screening, Melting curve analysis, Processing strategies, Quantitative polymerase chain reaction, Sample preparation, Polymerase chain reaction
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Natural Sciences
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URN: urn:nbn:se:ri:diva-39004DOI: 10.1016/B978-0-85709-801-6.00009-5Scopus ID: 2-s2.0-84942879976ISBN: 9780857098078 (print)ISBN: 9780857098016 (print)OAI: oai:DiVA.org:ri-39004DiVA, id: diva2:1324985
Available from: 2019-06-14 Created: 2019-06-14 Last updated: 2019-06-14Bibliographically approved

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Löfström, Charlotta

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CiteExportLink to record
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  • apa
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