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Enumeration of salmonellae in table eggs, pasteurized egg products, and egg-containing dishes by using quantitative real-time PCR
DTU Technical University of Denmark, Denmark.ORCID iD: 0000-0001-6492-1245
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2014 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 80, no 5, p. 1616-1622Article in journal (Refereed) Published
Abstract [en]

Salmonellae are a major cause of food-borne outbreaks in Europe, with eggs and egg products being identified as major sources. Due to the low levels of salmonellae in eggs and egg products, direct quantification is difficult. In the present study, enrichment quantitative real-time PCR (qPCR) was employed for enumeration of salmonellae in different matrices: table eggs, pasteurized egg products, and egg-containing dishes. Salmonella enterica serovar Enteritidis and S. enterica serovar Tennessee were used to artificially contaminate these matrices. The results showed a linear regression between the numbers of salmonellae and the quantification cycle (Cq) values for all matrices used, with the exception of pasteurized egg white. Standard curves were constructed by using both stationary-phase cells and heat-stressed cells, with similar results. Finally, this method was used to evaluate the fate of salmonellae in two egg-containing dishes, long egg and tiramisu, at abused refrigeration temperatures, and results indicated the growth of bacteria over a 1-week period. In conclusion, enrichment qPCR was shown to be reliable for enumeration of salmonellae in different egg products. © 2014, American Society for Microbiology.

Place, publisher, year, edition, pages
2014. Vol. 80, no 5, p. 1616-1622
Keywords [en]
article, bacterial load, egg, Europe, evaluation study, food control, isolation and purification, methodology, microbiology, pasteurization, real time polymerase chain reaction, Salmonella enterica, Eggs, Food Microbiology, Real-Time Polymerase Chain Reaction
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Natural Sciences
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URN: urn:nbn:se:ri:diva-39005DOI: 10.1128/AEM.03360-13Scopus ID: 2-s2.0-84894030200OAI: oai:DiVA.org:ri-39005DiVA, id: diva2:1324980
Available from: 2019-06-14 Created: 2019-06-14 Last updated: 2019-06-14Bibliographically approved

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Löfström, Charlotta

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