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Validation of a real-time PCR based method for detection of clostridium botulinum types C, D and their mosaic variants C-D and D-C in a multicenter collaborative trial
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2013 (English)In: Anaerobe, ISSN 1075-9964, E-ISSN 1095-8274, Vol. 22, p. 31-37Article in journal (Refereed) Published
Abstract [en]

Two real-time PCR arrays based on the GeneDisc® cycler platform (Pall-GeneDisc Technologies) were evaluated in a multicenter collaborative trial for their capacity to specifically detect and discriminate Clostridium botulinum types C, D and their mosaic variants C-D and D-C that are associated with avian and mammalian botulism. The GeneDisc® arrays developed as part of the DG Home funded European project 'AnibioThreat' were highly sensitive and specific when tested on pure isolates and naturally contaminated samples (mostly clinical specimen from avian origin). Results of the multicenter collaborative trial involving eight laboratories in five European Countries (two laboratories in France, Italy and The Netherlands, one laboratory in Denmark and Sweden), using DNA extracts issued from 33 pure isolates and 48 naturally contaminated samples associated with animal botulism cases, demonstrated the robustness of these tests. Results showed a concordance among the eight laboratories of 99.4%-100% for both arrays. The reproducibility of the tests was high with a relative standard deviation ranging from 1.1% to 7.1%. Considering the high level of agreement achieved between the laboratories these PCR arrays constitute robust and suitable tools for rapid detection of C.botulinum types C, D and mosaic types C-D and D-C. These are the first tests for C.botulinum C and D that have been evaluated in a European multicenter collaborative trial. © 2013 Elsevier Ltd.

Place, publisher, year, edition, pages
2013. Vol. 22, p. 31-37
Keywords [en]
Animal botulism, C.botulinum C and D, Evaluation trial, GeneDisc® array, bacterial DNA, article, bacterial strain, bacterium detection, botulism, Clostridium botulinum type C, Clostridium botulinum type D, controlled clinical trial, controlled study, Denmark, France, Italy, multicenter study, Netherlands, nonhuman, priority journal, real time polymerase chain reaction, reproducibility, Sweden, C. botulinum C and D, GeneDisc(®) array, Animals, Europe, Humans, Real-Time Polymerase Chain Reaction, Reproducibility of Results, Animalia, Aves, Clostridium botulinum, Mammalia
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Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-39015DOI: 10.1016/j.anaerobe.2013.05.002Scopus ID: 2-s2.0-84880731229OAI: oai:DiVA.org:ri-39015DiVA, id: diva2:1324815
Note

Funding details: European Commission; Funding text 1: This research was supported by the framework of the EU-project AniBioThreat (Grant Agreement: Home/2009/ISEC/AG/191 ) with the financial support from the Prevention of and Fight against Crime Programme of the European Union, European Commission – Directorate General Home Affairs. This publication reflects the views only of the author, and the European Commission cannot be held responsible for any use which may be made of the information contained therein. We also thank the DIM malinf Ile de France for the financial support of the project. Pia Engelsmann (DTU) is acknowledged for excellent technical assistance.

Available from: 2019-06-14 Created: 2019-06-14 Last updated: 2019-06-20Bibliographically approved

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Löfström, Charlotta

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