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The use of infrared thermography as a novel approach for real-time validation of PCR thermocyclers
DTU Technical University of Denmark, Denmark.ORCID iD: 0000-0001-6492-1245
2010 (English)In: Food Analytical Methods, ISSN 1936-9751, E-ISSN 1936-976X, Vol. 3, no 2, p. 116-119Article in journal (Refereed) Published
Abstract [en]

Validation of PCR thermocycler performance is crucial to obtain reliable results. In this study, infrared (IR) thermography was evaluated as a novel validation tool. After stabilisation, no significant difference in the temperatures recorded using thermography and a reference block-based system was found. By employing IR thermography, information about the length of the time until temperature stabilisation in the sample could be obtained. This study shows the potential of using IR thermography for validation of thermocyclers. © Springer Science + Business Media, LLC 2009.

Place, publisher, year, edition, pages
2010. Vol. 3, no 2, p. 116-119
Keywords [en]
Infrared Thermography, PCR, Thermal Cycler, Thermocycler, Validation
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-39029DOI: 10.1007/s12161-009-9103-2Scopus ID: 2-s2.0-77952828911OAI: oai:DiVA.org:ri-39029DiVA, id: diva2:1324739
Note

 Funding details: 036272; Funding text 1: Acknowledgement The authors thank the Nordic Joint Committee for Agricultural Research (NKJ) for the PhD grant to Hugo Ahlm Grønlund. This study was also supported by the European Union-funded integrated project BIOTRACER (contract no. 036272) under the sixth RTD Framework. Peter Rådström and Jeffrey Skiby are acknowledged for critical reading of the manuscript.

Available from: 2019-06-14 Created: 2019-06-14 Last updated: 2019-06-14Bibliographically approved

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Löfström, Charlotta

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