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Diagnostic PCR: Comparative sensitivity of four probe chemistries
DTU Technical University of Denmark, Denmark.ORCID iD: 0000-0001-6492-1245
2009 (English)In: Molecular and Cellular Probes, ISSN 0890-8508, E-ISSN 1096-1194, Vol. 23, no 3-4, p. 201-203Article in journal (Refereed) Published
Abstract [en]

Three probe chemistries: locked nucleic acid (LNA), minor groove binder (MGB) and Scorpion were compared with a TaqMan probe in a validated real-time PCR assay for detection of food-borne thermotolerant Campylobacter. The LNA probe produced significantly lower Ct-values and a higher proportion of positive PCR responses analyzing less than 150 DNA copies than the TaqMan probe. Choice of probe chemistry clearly has an impact on the sensitivity of PCR assays, and should be considered in an optimization strategy.

Place, publisher, year, edition, pages
2009. Vol. 23, no 3-4, p. 201-203
Keywords [en]
LNA, MGB, Probe, Real-time PCR, Scorpion, TaqMan, fluorescent dye, locked nucleic acid, article, Campylobacter, diagnostic value, DNA probe, gene probe, intermethod comparison, minor groove binder, nonhuman, priority journal, real time polymerase chain reaction, scorpion technology probe, sensitivity and specificity, DNA Probes, Food Microbiology, Polymerase Chain Reaction, Scorpiones
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-39031DOI: 10.1016/j.mcp.2009.02.003Scopus ID: 2-s2.0-67349207929OAI: oai:DiVA.org:ri-39031DiVA, id: diva2:1324720
Available from: 2019-06-14 Created: 2019-06-14 Last updated: 2019-06-14Bibliographically approved

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Löfström, Charlotta

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