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Functionalized deoxynucleotides and DNA primers for electrochemical diagnostics of disease predispostions
Sorbonne University, France.
Rovira i Virgili University, Spain.
RISE - Research Institutes of Sweden, ICT, Acreo. Linköping University, Sweden.ORCID iD: 0000-0001-6889-0351
Sorbonne University, France.
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2017 (English)In: ECS Transactions, Electrochemical Society Inc. , 2017, no 11, p. 1873-1883Conference paper, Published paper (Refereed)
Abstract [en]

Redox labeled DNAs are of increasing interest for the fabrication of next generation molecular tools. In the present work we are investigating the use of various redox labeled dNTPs, ddNTPs and DNA primers for use in detection of diseases. We have reported the use of Polyoxometalate (POM) labeled DNA primers and dNTPs for use in PCR and subsequently used for direct electrochemical detection of PCR products. The use of POM labeled DNAs in PCR enabled us to check the compatibility with polymerases and PCR incorporability of the modified DNAs. Furthermore we have investigated the solid-phase array based primer extension (é-PEX) with redox labelled ddNTPs (ferrocene (Fc), anthraquinone (AQ) phenothiazine (PTZ) and methylene blue (MB)) to prove the strategy of detection of single nucleotide polymorphisms using the labeled ddNTPs. This strategy will allow the development of cost-effective, rapid and user-friendly platform for the screening of known and unknown genetic mutations.

Place, publisher, year, edition, pages
Electrochemical Society Inc. , 2017. no 11, p. 1873-1883
Keywords [en]
Aromatic compounds, Chemical detection, Cost effectiveness, Diagnosis, DNA, Ketones, Nucleic acids, Organometallics, Anthraquinone (aq), ELectrochemical detection, Genetic mutations, Molecular tools, Polyoxometalates, Primer extension, Single nucleotide polymorphisms, User-friendly platforms, Polymerase chain reaction
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Other Engineering and Technologies not elsewhere specified
Identifiers
URN: urn:nbn:se:ri:diva-38130DOI: 10.1149/07711.1873ecstScopus ID: 2-s2.0-85030537182ISBN: 9781607685395 (print)OAI: oai:DiVA.org:ri-38130DiVA, id: diva2:1294447
Conference
231st ECS Meeting, 28 May 2017 through 1 June 2017
Note

Conference code: 130672; Export Date: 4 March 2019; Conference Paper

Available from: 2019-03-07 Created: 2019-03-07 Last updated: 2019-06-18Bibliographically approved

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Beni, Valerio

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