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Volume-amplified magnetic bioassay integrated with microfluidic sample handling and high-Tc SQUID magnetic readout
Chalmers University of Technology, Sweden.
RISE - Research Institutes of Sweden, ICT, Acreo.
Chalmers University of Technology, Sweden.
Uppsala University, Sweden.
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2017 (English)In: APL Bioengineering, Vol. 2, no 1, article id 016102Article in journal (Refereed) Published
Abstract [en]

A bioassay based on a high-Tc superconducting quantum interference device (SQUID) reading out functionalized magnetic nanoparticles (fMNPs) in a prototype microfluidic platform is presented. The target molecule recognition is based on volume amplification using padlock-probe-ligation followed by rolling circle amplification (RCA). The MNPs are functionalized with single-stranded oligonucleotides, which give a specific binding of the MNPs to the large RCA coil product, resulting in a large change in the amplitude of the imaginary part of the ac magnetic susceptibility. The RCA products from amplification of synthetic Vibrio cholera target DNA were investigated using our SQUID ac susceptibility system in microfluidic channel with an equivalent sample volume of 3 μl. From extrapolation of the linear dependence of the SQUID signal versus concentration of the RCA coils, it is found that the projected limit of detection for our system is about 1.0 × 105 RCA coils (0.2 × 10−18 mol), which is equivalent to 66 fM in the 3 μl sample volume. This ultra-high magnetic sensitivity and integration with microfluidic sample handling are critical steps towards magnetic bioassays for rapid detection of DNA and RNA targets at the point of care.

Place, publisher, year, edition, pages
American Institute of Physics , 2017. Vol. 2, no 1, article id 016102
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-36362DOI: 10.1063/1.4999713OAI: oai:DiVA.org:ri-36362DiVA, id: diva2:1266254
Available from: 2018-11-27 Created: 2018-11-27 Last updated: 2019-06-27Bibliographically approved

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Publisher's full texthttps://doi.org/10.1063/1.4999713

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Blomgren, JakobJohansson, Christer

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